Identification of β2-microgloblin as a candidate for early diagnosis of imaging-invisible hepatocellular carcinoma in patient with liver cirrhosis

  • Authors:
    • Yumi Saito
    • Nobuyuki Oba
    • Syuta Nishinakagawa
    • Yasuhiro Mizuguchi
    • Tatsuya Kojima
    • Kazuhiro Nomura
    • Tetsuya Nakatsura
  • View Affiliations

  • Published online on: May 1, 2010     https://doi.org/10.3892/or_00000767
  • Pages: 1325-1330
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Abstract

Glypican-3 (GPC3) is overexpressed in hepatocellular carcinoma (HCC) but not in chronic hepatitis (CH) and liver cirrhosis (LC). We have reported the possibility of GPC3-specific cytotoxic T lymphocytes (CTLs) serving as a marker for the early diagnosis of imaging invisible HCC. In this study, to identify new early diagnostic biomarker of imaging invisible HCC, we analyzed plasma of healthy donors and patients with CH, LC and HCC using surface-enhanced laser desorption-ionaization time-of-flight mass spectrometry (SELDI-TOF-MS). The intensities of four peaks were significantly increased in HCC patients compared with healthy donors. Two of these four peaks were significantly higher in CH and LC patients with GPC3-specific CTLs than in those without. One peak (11.7 kDa) was predicted to be β2-microglobulin (β2-MG) by molecular mass. There was a correlation between concentration of β2-MG by latex agglutination immunoassay in plasma and peak intensity using SELDI-TOF-MS. The 11.7 kDa protein was fractionated by gel filtration and was identified as β2-MG by Western blot analysis. These results suggest that the level of β2-MG in plasma from patients with CH and LC could be a useful marker for the early diagnosis of imaging invisible HCC, however further investigation is needed.

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May 2010
Volume 23 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Saito Y, Oba N, Nishinakagawa S, Mizuguchi Y, Kojima T, Nomura K and Nakatsura T: Identification of β2-microgloblin as a candidate for early diagnosis of imaging-invisible hepatocellular carcinoma in patient with liver cirrhosis. Oncol Rep 23: 1325-1330, 2010.
APA
Saito, Y., Oba, N., Nishinakagawa, S., Mizuguchi, Y., Kojima, T., Nomura, K., & Nakatsura, T. (2010). Identification of β2-microgloblin as a candidate for early diagnosis of imaging-invisible hepatocellular carcinoma in patient with liver cirrhosis. Oncology Reports, 23, 1325-1330. https://doi.org/10.3892/or_00000767
MLA
Saito, Y., Oba, N., Nishinakagawa, S., Mizuguchi, Y., Kojima, T., Nomura, K., Nakatsura, T."Identification of β2-microgloblin as a candidate for early diagnosis of imaging-invisible hepatocellular carcinoma in patient with liver cirrhosis". Oncology Reports 23.5 (2010): 1325-1330.
Chicago
Saito, Y., Oba, N., Nishinakagawa, S., Mizuguchi, Y., Kojima, T., Nomura, K., Nakatsura, T."Identification of β2-microgloblin as a candidate for early diagnosis of imaging-invisible hepatocellular carcinoma in patient with liver cirrhosis". Oncology Reports 23, no. 5 (2010): 1325-1330. https://doi.org/10.3892/or_00000767