PRE-CONGRESS WORKSHOP
Next-Generation Sequencing – Library construction and quality control (theoretical)
3-4 October 2017
Metropolitan Hotel
Athens, Greece
Deadline for registrations: 27 September 2017
WORKSHOP
Title: Next-Generation Sequencing – Library construction and quality control (theoretical)
Description: This course gives an introduction to massively parallel sequencing (also called Next Generation Sequencing, NGS), and its many applications. The course consists of a theoretical part, which will focus on considerations for the NGS experiment design, the different sequencing platforms, quality control of samples, library preparation techniques, and quantification of libraries for sequencing. The course also includes practical parts where the participants will prepare libraries and perform quality control and compare libraries.
The course contains:
- Introduction to NGS:
- The history of DNA sequencing
- What are the advantages with NGS? Disadvantages?
- What is the difference between microarray and NGS?
Applications:
- What are the possibilities for analysis of the genome? Transcriptome? Epigenome? Metagenome?
- RNA sequencing; investigating differential expression, splice variants, novel transcripts and much more.
- DNA sequencing; finding Single-Nucleotide Polymorphisms (SNPs), Copy Number Variants (CNVs), structural variations and more.
- NGS for epigenetic studies; analyzing the methylation state of DNA or discovering protein-binding sites in DNA.
Sample preparation and quality control:
- How to prepare samples for NGS.
- Which starting material can be used for NGS?
- Which quality controls are included in the experimental workflow?
- How to choose the right library prep protocol.
- Practical experiment to learn the basics of library preparation and quality control.
Instrumentation:
- Which platforms are available?
- How do they work?
- Which platform is suitable for my experiment?
Experimental design:
- What to think about when designing your NGS experiment.
- How much to sequence?
- How to calculate coverage and depth?
- How many samples can I multiplex?
The course is held by TATAA Biocenter, that has been a qPCR and NGS course provider since 2001 and have trained thousands of satisfied customers. We want to share our knowledge with you too!
Course leader: Filip Stern, PhD, NGS Service Manager, TATAA Biocenter
Filip Cuklev Stern received his PhD in Molecular Biology from the University of Gothenburg in 2012. He has since worked with Next Generation Sequencing and started at TATAA Biocenter in 2015 as NGS service manager, which implies holding and developing courses in NGS. He is further involved in commissioned services at TATAA and is project manager for customer projects within the NGS field, but also qPCR. Additionally, Filip functions as application specialist and has thereby experience from a broad spectrum of different library preparations.
Place: Metropolitan Hotel, Athens, Greece
Date: 3-4th of October 2017
Time: 9:00 a.m. - 5:00 p.m.
Registration date-time: October 3, 2017; from 8:00 a.m. to 9:00 a.m.
Registration fees: 590 EUR (Academic delegates), 690 EUR (Industrial delegates)
For questions or any further requests please send an email to workshops@spandidos-publications.com.
