MicroRNA Analysis (theoretical)
18-19 September 2018
The New Athens Metropolitan Hotel
Deadline for registrations: 31 July 2018
Learn how to extract and analyze miRNA with appropriate techniques!
Title: MicroRNA Analysis
Target audience: Researchers interested in analyzing microRNA.
Entrance qualifications: Basic knowledge about microRNA and molecular analyses.
Description: This course (2 days miRNA analysis) introduces miRNA including its variant forms. Methods to extract and purify microRNA and prepare it for analysis including quality control. Methods for microRNA expression analysis are presented and compared, to learn how to too choose the best method for each study. Analysis of microRNA data is also discussed including challenging normalization.
The course contains:
- MicroRNA background including biogenesis and function
- MicroRNA extraction – sampling, miRNA stability, extraction kit comparison
- Quality Control of miRNA
- MicroRNA quantification with microarray, nanoString, NGS and RT -qPCR methods
- Comparison of miRNA quantification methods
- Hands-on lab testing quality and quantity of miRNA
- Experimental design and data analysis
- RT-qPCR normalization of miRNA data
- Introduction to NGS data analysis
- MicroRNA and liquid biopsies – challenges of miRNA analysis in biofluids
- Introduction to functional studies
- Hands-on lab of miRNA RT-qPCR analysis including data analysis in Genex
The course is held by TATAA Biocenter, that has been a qPCR and NGS course provider since 2001 and have trained thousands of satisfied customers. We want to share our knowledge with you too!
Course leader: Dr. Robert Sjöback, RnD Manager, TATAA Biocenter
Robert Sjöback received his PhD in Physical Chemistry from Chalmers University of Technology, Gothenburg, in 1997. Sjöback worked several years with research and product development of PCR and ELISA based kits for food diagnostics. Since 2005 he works at TATAA Biocenter responsible for the TATAA Biocenter’s research and product development and as instructor in the company training program. Focus of research is on miRNA analysis, both by development of the two-tailed RT-qPCR technique and by participation in miRNA oriented EU H2020 funded research projects.
Place: The New Athens Metropolitan Hotel, Athens, Greece
Date: 18-19th of September 2018
Time: 9:00 a.m. - 5:00 p.m.
Registration date-time: September 18, 2018; from 8:00 a.m. to 9:00 a.m.
Registration fees: 590 EUR (Academic delegates), 690 EUR (Industrial delegates)
For questions or any further requests please send an email to firstname.lastname@example.org.