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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

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Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Detection of circulating tumour cells on mRNA levels with established breast cancer cell lines

  • Authors:
    • Michael Zebisch
    • Alexandra C. Kölbl
    • Ulrich Andergassen
    • Stephan Hutter
    • Julia Neugebauer
    • Verena Engelstädter
    • Maria Günthner-Biller
    • Udo Jeschke
    • Klaus Friese
    • Brigitte Rack
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynaecology, Ludwig-Maximilians University of Munich, Munich, Germany
  • Pages: 231-234
    |
    Published online on: December 5, 2012
       https://doi.org/10.3892/br.2012.45
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Abstract

Circulating tumour cells were detected and quantified by real-time polymerase chain reaction (PCR) in peripheral blood, based on the fact that the expression of certain genes is upregulated in tumour tissues in comparison to surrounding blood cells. Calibration curves showing gene expression as functions of the number of tumour cells within a blood sample were prepared. Blood samples were therefore spiked with cells of breast cancer cell lines, RNA was extracted, transcribed to complementary DNA (cDNA) and used in real‑time PCR reaction on the Cytokeratins (CK) 8, 18 and 19. Calibration curves were generated by Microsoft™ Excel®. Relative quanti­fication curves of gene expression in different breast cancer cell lines showed no unitary tendencies. The oscillations in the relative quantification curves of gene expression suggested an occurrence of immunological effects, leading to an apparent agglutination of added tumour cells together with the blood cells of the sample. Thus, strategies to obtain evaluable results should be considered.
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Copy and paste a formatted citation
Spandidos Publications style
Zebisch M, Kölbl AC, Andergassen U, Hutter S, Neugebauer J, Engelstädter V, Günthner-Biller M, Jeschke U, Friese K, Rack B, Rack B, et al: Detection of circulating tumour cells on mRNA levels with established breast cancer cell lines. Biomed Rep 1: 231-234, 2013.
APA
Zebisch, M., Kölbl, A.C., Andergassen, U., Hutter, S., Neugebauer, J., Engelstädter, V. ... Rack, B. (2013). Detection of circulating tumour cells on mRNA levels with established breast cancer cell lines. Biomedical Reports, 1, 231-234. https://doi.org/10.3892/br.2012.45
MLA
Zebisch, M., Kölbl, A. C., Andergassen, U., Hutter, S., Neugebauer, J., Engelstädter, V., Günthner-Biller, M., Jeschke, U., Friese, K., Rack, B."Detection of circulating tumour cells on mRNA levels with established breast cancer cell lines". Biomedical Reports 1.2 (2013): 231-234.
Chicago
Zebisch, M., Kölbl, A. C., Andergassen, U., Hutter, S., Neugebauer, J., Engelstädter, V., Günthner-Biller, M., Jeschke, U., Friese, K., Rack, B."Detection of circulating tumour cells on mRNA levels with established breast cancer cell lines". Biomedical Reports 1, no. 2 (2013): 231-234. https://doi.org/10.3892/br.2012.45
Copy and paste a formatted citation
x
Spandidos Publications style
Zebisch M, Kölbl AC, Andergassen U, Hutter S, Neugebauer J, Engelstädter V, Günthner-Biller M, Jeschke U, Friese K, Rack B, Rack B, et al: Detection of circulating tumour cells on mRNA levels with established breast cancer cell lines. Biomed Rep 1: 231-234, 2013.
APA
Zebisch, M., Kölbl, A.C., Andergassen, U., Hutter, S., Neugebauer, J., Engelstädter, V. ... Rack, B. (2013). Detection of circulating tumour cells on mRNA levels with established breast cancer cell lines. Biomedical Reports, 1, 231-234. https://doi.org/10.3892/br.2012.45
MLA
Zebisch, M., Kölbl, A. C., Andergassen, U., Hutter, S., Neugebauer, J., Engelstädter, V., Günthner-Biller, M., Jeschke, U., Friese, K., Rack, B."Detection of circulating tumour cells on mRNA levels with established breast cancer cell lines". Biomedical Reports 1.2 (2013): 231-234.
Chicago
Zebisch, M., Kölbl, A. C., Andergassen, U., Hutter, S., Neugebauer, J., Engelstädter, V., Günthner-Biller, M., Jeschke, U., Friese, K., Rack, B."Detection of circulating tumour cells on mRNA levels with established breast cancer cell lines". Biomedical Reports 1, no. 2 (2013): 231-234. https://doi.org/10.3892/br.2012.45
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