DNMT3A -448A>G polymorphism and the risk for hepatocellular carcinoma

Zhao,Chengcheng; Yan,Feng; Wu,Huazhang; Qiao,Fengchang; Qiu,Xuemei; Fan,Hong

doi:10.3892/br.2013.121

July-August 2013 Volume 1 Issue 4

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July-August 2013 Volume 1 Issue 4

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Article

DNMT3A -448A>G polymorphism and the risk for hepatocellular carcinoma

Authors:
- Chengcheng Zhao
- Feng Yan
- Huazhang Wu
- Fengchang Qiao
- Xuemei Qiu
- Hong Fan
View Affiliations / Copyright

Affiliations: Department of Medical Genetics and Developmental Biology, Medical School of Southeast University and Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Nanjing, Jiangsu 210009, P.R. China, The Jiangsu Cancer Hospital, Nanjing, Jiangsu 210009, P.R. China
Pages: 664-668
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Published online on: May 30, 2013

https://doi.org/10.3892/br.2013.121
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Abstract

DNA-methyltransferase (DNMT) 3A plays a significant role in carcinogenesis. Findings of a previous study suggested an association between the DNMT3A -448A>G single‑nucleotide polymorphism (SNP) and susceptibility to gastric cancer (GC) and colorectal cancer (CRC). Hepatocellular carcinoma (HCC) is a common malignancy, with a similar expression pattern to GC. The aim of this case-control study was to determine whether there is an association between DNMT3A gene polymorphism and susceptibility to HCC. Real-time quantitive PCR (qPCR) was employed to detect DNMT3A expression in tumor and non-cancer liver tissue from 13 HCC patients. An increased expression of DNMT3A was detected, as well as -448A>G polymorphisms of DNMT3A promoter by polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP), confirmed by sequencing. The distribution of -448A>G polymorphisms was examined in 108 HCC patients and 225 healthy controls who were matched for age and gender. The association of -448A>G polymorphisms of DNMT3A and the risk of HCC was evaluated by stratified analysis according to the patient's age and gender. The allele frequency of -448A among HCC patients and the controls was 24.07 vs. 24.22%, respectively. The frequency of genotypes GG, AG, AA was 55.56 vs. 56.89%, 40.74 vs. 37.78%, 3.7 vs. 5.33%, respectively. The results indicated that -448A>G is not associated with susceptibility to HCC, although -448A>G is a functional single‑nucleotide polymorphism (SNP) and increased the expression of DNMT3A in HCC cases. Findings of the present study suggested that the DNMT3A -448A>G polymorphism is an insufficient biomarker to predict the susceptibility to HCC.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

DNMT3A -448A>G polymorphism and the risk for hepatocellular carcinoma

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