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Article

Expression of set is downregulated by rapamycin in human colorectal cancer cells

  • Authors:
    • Xiaoxia Wen
    • Yao Chen
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy, College of Basic and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China
  • Pages: 727-730
    |
    Published online on: July 11, 2013
       https://doi.org/10.3892/br.2013.133
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Abstract

The purpose of this study was to determine the mechanism through which rapamycin treatment affects the expression of the set gene in human colorectal adenocarcinoma cells. The effect of rapamycin treatment on set expression was evaluated by assessing the mRNA and protein expression of set in the SW480 and LoVo human colon carcinoma cell lines following treatment with rapamycin by quantitative polymerase chain reaction (qPCR) and western blot analysis, respectively. Our results demonstrated that the mRNA and protein levels of set were significantly decreased subsequent to rapamycin treatment in the two cell lines, indicating that set expression may be downregulated by rapamycin in human colorectal adenocarcinoma cells. Our findings suggested that the mammalian target of rapamycin signaling pathway may play a role in tumorigenesis through the regulation of the set gene.
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Copy and paste a formatted citation
Spandidos Publications style
Wen X and Chen Y: Expression of set is downregulated by rapamycin in human colorectal cancer cells. Biomed Rep 1: 727-730, 2013.
APA
Wen, X., & Chen, Y. (2013). Expression of set is downregulated by rapamycin in human colorectal cancer cells. Biomedical Reports, 1, 727-730. https://doi.org/10.3892/br.2013.133
MLA
Wen, X., Chen, Y."Expression of set is downregulated by rapamycin in human colorectal cancer cells". Biomedical Reports 1.5 (2013): 727-730.
Chicago
Wen, X., Chen, Y."Expression of set is downregulated by rapamycin in human colorectal cancer cells". Biomedical Reports 1, no. 5 (2013): 727-730. https://doi.org/10.3892/br.2013.133
Copy and paste a formatted citation
x
Spandidos Publications style
Wen X and Chen Y: Expression of set is downregulated by rapamycin in human colorectal cancer cells. Biomed Rep 1: 727-730, 2013.
APA
Wen, X., & Chen, Y. (2013). Expression of set is downregulated by rapamycin in human colorectal cancer cells. Biomedical Reports, 1, 727-730. https://doi.org/10.3892/br.2013.133
MLA
Wen, X., Chen, Y."Expression of set is downregulated by rapamycin in human colorectal cancer cells". Biomedical Reports 1.5 (2013): 727-730.
Chicago
Wen, X., Chen, Y."Expression of set is downregulated by rapamycin in human colorectal cancer cells". Biomedical Reports 1, no. 5 (2013): 727-730. https://doi.org/10.3892/br.2013.133
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