Combined effects of simvastatin and fibroblast growth factor‑2 on the proliferation and differentiation of preosteoblasts

Park,Jun‑Beom

doi:10.3892/br.2013.137

September-October 2013 Volume 1 Issue 5

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September-October 2013 Volume 1 Issue 5

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Article

Combined effects of simvastatin and fibroblast growth factor‑2 on the proliferation and differentiation of preosteoblasts

Authors:
- Jun‑Beom Park
View Affiliations / Copyright

Affiliations: Department of Periodontics, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137‑701, Republic of Korea
Pages: 812-814
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Published online on: July 17, 2013

https://doi.org/10.3892/br.2013.137
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Abstract

Simvastatin reportedly promotes osteoblastic and inhibits osteoclastic activity. It increases bone formation when injected subcutaneously over the calvaria in mice. It also increases cancellous bone volume in rats following oral administration. Fibroblast growth factor‑2 (FGF‑2), a member of the FGF family, is expressed by cells of the osteoblastic lineage. FGF‑2 promotes osteoblast proliferation and it is secreted during the healing process of fractures or at bone surgery sites. FGF‑2 reportedly regulates bone formation and osteoblast differentiation. In this study, the combined effects of simvastatin and FGF‑2 on the proliferation and differentiation of preosteoblasts were investigated and an alkaline phosphatase (ALP) activity test was performed to assess the differentiation. Moreover, the expression of proteins associated with bone formation were measured using western blot analysis. The results demonstrated that the protein content of the cultures grown in osteogenic differentiation media in the presence of FGF‑2 at a concentration of 20 ng/ml was higher compared to that of the untreated control cultures. ALP activity was decreased when cells were treated with FGF‑2 (2 and 20 ng̸ml) and increased when cells were treated with simvastatin. The cultures grown in the presence of 1 µM of simvastatin and 2 ng̸ml of FGF‑2 exhibited increased ALP activity when compared to that in the 2 ng̸ml FGF‑2‑only group. The combination of 1.0 µM simvastatin and 2 ng̸ml FGF‑2 achieved a higher estrogen receptor‑α expression compared to the 2 ng̸ml FGF‑2‑only group. Within the limits of this study, simvastatin enhanced osteoblast differentiation. However, the combined treatment with simvastatin and FGF‑2 did not exert synergistic effects on osteoblast differentiation under the current experimental conditions. Future studies are required to evaluate divergent conditions and determine the selective timing and optimal dosage for the delivery of the agents.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Combined effects of simvastatin and fibroblast growth factor‑2 on the proliferation and differentiation of preosteoblasts

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