Recombinant expression, different downstream processing of the disulfide‑rich anti‑tumor peptide Ranpirnase and its effect on the growth of human glioma cell line SHG‑44

Wang,Xiao‑Min; Guo,Zhan‑Yun

doi:10.3892/br.2013.138

September-October 2013 Volume 1 Issue 5

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September-October 2013 Volume 1 Issue 5

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Article

Recombinant expression, different downstream processing of the disulfide‑rich anti‑tumor peptide Ranpirnase and its effect on the growth of human glioma cell line SHG‑44

Authors:
- Xiao‑Min Wang
- Zhan‑Yun Guo
View Affiliations / Copyright

Affiliations: Institute of Protein Research, College of Life Sciences and Technology, Tongji University, Shanghai 200092, P.R. China
Pages: 747-750
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Published online on: July 17, 2013

https://doi.org/10.3892/br.2013.138
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Abstract

Ranpirnase (Onconase) is a frogspawn‑derived disulfide‑rich peptide with ribonuclease activity that may be used for tumor treatment. In the present study, we established an efficient approach for preparing mature ranpirnase which may be used for research and therapeutic purposes. The designed ranpirnase precursors carried a 6xHis‑tag and were recombinantly expressed in Escherichia coli. After S‑sulfonation, the precursors were purified by immobilized metal‑ion affinity chromatography. Following removal of the tag by aminopeptidase cleavage, cyclization and in vitro oxidative refolding, the mature ranpirnase was obtained with considerable yield, and the yield of mature ranpirnase was ~50‑60 mg per liter cultures. In addition, ranpirnase inhibited the growth of human glioma cells SHG‑44 in a dose‑dependent manner. Thus the present study has provided an efficient approach for the preparation of active ranpirnase and its analogues for future studies.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Recombinant expression, different downstream processing of the disulfide‑rich anti‑tumor peptide Ranpirnase and its effect on the growth of human glioma cell line SHG‑44

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