Effects of quercetin on CDK4 mRNA and protein expression in A549 cells infected by H1N1

Wan,Qiaofeng; Wang,Hao; Lin,Yuan; Gu,Ligang; Han,Mei; Yang,Zhiwei; Zhang,Yanli; Ma,Rui; Wang,Li; Wang,Zhisheng

doi:10.3892/br.2013.141

September-October 2013 Volume 1 Issue 5

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September-October 2013 Volume 1 Issue 5

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Article

Effects of quercetin on CDK4 mRNA and protein expression in A549 cells infected by H1N1

Authors:
- Qiaofeng Wan
- Hao Wang
- Yuan Lin
- Ligang Gu
- Mei Han
- Zhiwei Yang
- Yanli Zhang
- Rui Ma
- Li Wang
- Zhisheng Wang
View Affiliations / Copyright

Affiliations: Department of Pathogen Biology and Immunology, Basic Medical Science College, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China, Key Laboratory of Antivirus of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, P.R. China
Pages: 766-770
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Published online on: July 19, 2013

https://doi.org/10.3892/br.2013.141
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Abstract

This study was conducted to investigate the effects of quercetin on the expression of cyclin‑dependent kinase (CDK4) mRNA and protein in A549 lung epithelial tumor cells infected by H1N1. First, the Thiazolyl Blue Tetrazolium Bromide (MTT) method was used to determine H1N1 virulence, quercetin cytotoxicity and inhibition of the cytopathic effect of H1N1 on A549 cells by quercetin. Subsequently, 100 TCID50 H1N1 was used to infect A549 cells for 2 h prior to culture in maintenance media containing 10 mg̸l quercetin. After 4, 12, 24 and 48 h of culture, the cells were collected and total RNA and protein were extracted. Fluorescent quantitative polymerase chain reaction and western blot analysis were then performed to assess the expression of CDK4 mRNA and protein. The experiment demonstrated that the TCID50 of H1N1 in A549 cells was 10‑4.75, the maximum non‑toxic concentration of quercetin in A549 cells was 30‑60 mg̸l and the minimum effective concentration of quercetin for the inhibition of the H1N1 cytopathic effect on A549 cells was 10 mg/l. The results indicated that quercetin may significantly inhibit CDK4 mRNA and protein overexpression caused by H1N1 within 4‑48 h. In conclusion, quercetin may protect against H1N1 infection by effectively reducing the mRNA and protein expression of CDK4 caused by H1N1 infection.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effects of quercetin on CDK4 mRNA and protein expression in A549 cells infected by H1N1

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