Forkhead box m1 mRNA expression of lung of preterm and term rabbits

Chang,Ji‑Young; Hahn,Won‑Ho; Kim,Chang‑Ryul; Bae,Chong‑Woo

doi:10.3892/br.2013.174

November-December 2013 Volume 1 Issue 6

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November-December 2013 Volume 1 Issue 6

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Article

Forkhead box m1 mRNA expression of lung of preterm and term rabbits

Authors:
- Ji‑Young Chang
- Won‑Ho Hahn
- Chang‑Ryul Kim
- Chong‑Woo Bae
View Affiliations / Copyright

Affiliations: Department of Pediatrics, Kyung Hee University Hospital at Gangdong, Seoul 134727, Republic of Korea, Department of Pediatrics, School of Medicine, Soon Chun Hyang University, Cheonan 330721, Republic of Korea, Department of Pediatrics, Hanyang University Medical Center, Guri 471701, Republic of Korea
Pages: 850-854
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Published online on: September 25, 2013

https://doi.org/10.3892/br.2013.174
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Abstract

Previous studies on forkhead box m1 (Foxm1) of mice demonstrated the correlation between this gene and lung maturation. However, no study has been conducted on human Foxm1 with regard to lung maturation. The aim of this study was to compare the mRNA expression of surfactant protein (SP)‑A, ‑B, ‑C and Foxm1 gene of preterm rabbits to that of full‑term ones and to determine the association between Foxm1 and lung maturation. New Zealand white rabbits were grouped according to gestational age. Cesarean sections were carried out after rabbits were divided into two groups of 30‑31 days of gestation (term group, n=18) and 26‑27 days of gestation (preterm group, n=18). mRNA expression levels of SP‑A, ‑B, ‑C and Foxm1 were compared by using quantitative real‑time reverse transcription‑polymerase chain reaction (qRT‑PCR). The relative ratios of SP‑A, ‑B and ‑C mRNA expression levels of the preterm to term groups were 0.380, 0.563 and 0.448:1, respectively, on qRT‑PCR. By contrast, Foxm1 expression was increased in the preterm group and its relative expression ratio to the term group was 2.166:1 for RT‑PCR and qRT‑PCR, which was double that of the Foxm1 gene in the term group. Moreover, a significant correlation between the expressions of these genes was found. Foxm1 is considered to be an important gene required for the lung maturation of preterm rabbits in correlation with SP genes.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Forkhead box m1 mRNA expression of lung of preterm and term rabbits

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