Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Biomedical Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 2049-9434 Online ISSN: 2049-9442
Journal Cover
January-February 2014 Volume 2 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-February 2014 Volume 2 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Direct regulation of vascular smooth muscle contraction by mastoparan‑7

  • Authors:
    • Grzegorz Grześk
    • Bartosz Malinowski
    • Elżbieta Grześk
    • Michał Wiciński
    • Katarzyna Szadujkis‑Szadurska
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology and Therapeutics, Collegium Medicum, Nicolaus Copernicus University, 85094 Bydgoszcz, Poland, Department of Pediatric Hematology and Oncology, Collegium Medicum, Nicolaus Copernicus University, 85094 Bydgoszcz, Poland
  • Pages: 34-38
    |
    Published online on: October 4, 2013
       https://doi.org/10.3892/br.2013.179
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Mastoparan‑7 (mas‑7) is a basic tetradecapeptide isolated from wasp venom, which activates guanine nucleotide-binding regulatory proteins (G‑proteins) and stimulates apoptosis. in smooth muscle cells, mas‑7 leads to an increase in the perfusion pressure. The main aim of this study was to evaluate the physiological effect of the direct stimulation of G‑proteins in comparison to the typical stimulation of receptors in vascular smooth muscle cells (VSMCs). Experiments were performed on the isolated and perfused tail artery of Wistar rats. The contraction force in our model was measured by an increased level of perfusion pressure with a constant flow. The concentration response curves (CRCs) obtained for mas‑7 were sigmoidal. In comparison to the curves for phenylephrine and vasopressin, the mas‑7 curve was significantly shifted to the right with a significant reduction in maximal response. Mas‑7 significantly increased the perfusion pressure for the intra‑ and extracellular calcium (Ca2+) influx to the cytoplasm. The presence of the pertussis toxin (PT) did not affect the mas‑7‑induced contraction. In comparison to phenylephrine and vasopressin, all the values of perfusion pressure following stimulation of the G‑proteins by mas‑7 were significantly lower. The results of our experiments suggested that mas‑7 significantly induces the contraction of VSMCs. The binding site for mas‑7 is different from that for PT; thus, PT does not affect VSMC contraction. The tissue effect of this stimulation is comparable to the stimulatory effect of partial agonists. Current knowledge regarding the apoptosis pathway reveals the significance of Ca2+ ions involved in this process. Therefore, mas‑7 may induce apoptosis through an increase in the cytoplasmic Ca2+ concentration; however, the use of this mechanism in anticancer therapy must be preceded by a molecule modification that eliminates the vasoconstrictive effect.
View Figures

Figure 1

Figure 2

View References

1 

King TP, Jim SY and Wittkowski KM: Inflammatory role of two venom components of yellow jackets (Vespula vulgaris): a mast cell degranulating peptide mastoparan and phospholipase A1. Int Arch Allergy Immunol. 131:25–32. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Hoshina MM, Santos LD, Palma MS and Marin-Morales MA: Cytotoxic, genotoxic/antigenotoxic and mutagenic/antimutagenic effects of the venom of the wasp Polybia paulista. Toxicon. 72:64–70. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Yordanova ZP, Woltering EJ, Kapchina-Toteva VM and Iakimova ET: Mastoparan-induced programmed cell death in the unicellular alga Chlamydomonas reinhardtii. Ann Bot. 111:191–205. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Lin CH, Hou RF, Shyu CL, Shia WY, Lin CF and Tu WC: In vitro activity of mastoparan-AF alone and in combination with clinically used antibiotics against multiple-antibiotic-resistant Escherichia coli isolates from animals. Peptides. 36:114–120. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Sowa NA, Street SE, Vihko P and Zylka MJ: Prostatic acid phosphatase reduces thermal sensitivity and chronic pain sensitization by depleting phosphatidylinositol 4,5-bisphosphate. J Neurosci. 30:10282–10293. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Hajnoczky G, Davies E and Madesh M: Calcium signaling and apoptosis. Biochem Biophys Res Commun. 304:445–454. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Newmeyer DD and Ferguson-Miller S: Mitochondria: releasing power for life and unleashing the machineries of death. Cell. 112:481–490. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Grześk G, Wiciński M, Malinowski B, Grześk E, Manysiak S, Odrowąż-Sypniewska G, Darvish N and Bierwagen M: Calcium blockers inhibit cyclosporine A-induced hyperreactivity of vascular smooth muscle cells. Mol Med Rep. 5:1469–1474. 2012.PubMed/NCBI

9 

Grześk G, Kozinski M, Navarese EP, Krzyzanowski M, Grześk E, Kubica A, Siller-Matula JM, Castriota F and Kubica J: Ticagrelor, but not clopidogrel and prasugrel, prevents ADP-induced vascular smooth muscle cell contraction: a placebo-controlled study in rats. Thromb Res. 130:65–69. 2012.PubMed/NCBI

10 

Kanagy NL and Webb RC: Enhanced vascular reactivity to mastoparan, a G protein activator, in genetically hypertensive rats. Hypertension. 23:946–950. 1994. View Article : Google Scholar : PubMed/NCBI

11 

Birnbaumer L: The discovery of signal transduction by G proteins: a personal account and an overview of the initial findings and contributions that led to our present understanding. Biochim Biophys Acta. 1768:756–771. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Cotecchia S: The α1-adrenergic receptors: diversity of signaling networks and regulation. J Recept Signal Transduct Res. 30:410–419. 2010.

13 

Bylund DB, et al: Adrenoceptors. The IUPHAR Compendium of Receptor Characterization and Classification. 2nd edition. IUPHAR Media; London: pp. 88–103. 2000

14 

Higashijima T, Burnier J and Ross EM: Regulation of Giand Goby mastoparan, related amphiphilic peptides, and hydrophobic amines. Mechanism and structural determinants of activity. J Biol Chem. 265:14176–14186. 1990.

15 

Perianin A and Snyderman R: Mastoparan, a wasp venom peptide, indentifies two discrete mechanisms for elevating cytosolic calcium and inositol triphosphates in human polymorphonuclear leukocytes. J Immunol. 143:1669–1673. 1989.

16 

Dostal DE, Murahashi T and Peach MJ: Regulation of cytosolic calcium by angiotensins in vascular smooth muscle. Hypertension. 15:815–822. 1990. View Article : Google Scholar : PubMed/NCBI

17 

Argiolas A and Pisano JJ: Facilitation of phospholipase A2 activity by mastoparans, a new class of mast cell degranulating peptides from wasp venom. J Biol Chem. 258:13697–13702. 1983.PubMed/NCBI

18 

Wallace MA and Carter HR: Effects of the wasp venom peptide, mastoparan, on a phosphoinositide-specific phospholipase C purified from rabbit brain membranes. Biochim Biophys Acta. 1006:311–316. 1989. View Article : Google Scholar : PubMed/NCBI

19 

Hiramatsu Y, Horn VJ, Baum BJ and Ambudkar IS: Characterization of polyphosphoinositide-specific phospholipase C in rat parotid gland membranes. Arch Biochem Biophys. 297:368–376. 1992. View Article : Google Scholar : PubMed/NCBI

20 

Grześk G and Szadujkis-Szadurski L: Modyfikacja reaktywnosci receptorow α-adrenergicznych przez 8Br-cGMP i angiotensyne II. Ann Acad Med Bydg. 17:5–10. 2003.(In Polish).

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Grześk G, Malinowski B, Grześk E, Wiciński M and Szadujkis‑Szadurska K: Direct regulation of vascular smooth muscle contraction by mastoparan‑7. Biomed Rep 2: 34-38, 2014.
APA
Grześk, G., Malinowski, B., Grześk, E., Wiciński, M., & Szadujkis‑Szadurska, K. (2014). Direct regulation of vascular smooth muscle contraction by mastoparan‑7. Biomedical Reports, 2, 34-38. https://doi.org/10.3892/br.2013.179
MLA
Grześk, G., Malinowski, B., Grześk, E., Wiciński, M., Szadujkis‑Szadurska, K."Direct regulation of vascular smooth muscle contraction by mastoparan‑7". Biomedical Reports 2.1 (2014): 34-38.
Chicago
Grześk, G., Malinowski, B., Grześk, E., Wiciński, M., Szadujkis‑Szadurska, K."Direct regulation of vascular smooth muscle contraction by mastoparan‑7". Biomedical Reports 2, no. 1 (2014): 34-38. https://doi.org/10.3892/br.2013.179
Copy and paste a formatted citation
x
Spandidos Publications style
Grześk G, Malinowski B, Grześk E, Wiciński M and Szadujkis‑Szadurska K: Direct regulation of vascular smooth muscle contraction by mastoparan‑7. Biomed Rep 2: 34-38, 2014.
APA
Grześk, G., Malinowski, B., Grześk, E., Wiciński, M., & Szadujkis‑Szadurska, K. (2014). Direct regulation of vascular smooth muscle contraction by mastoparan‑7. Biomedical Reports, 2, 34-38. https://doi.org/10.3892/br.2013.179
MLA
Grześk, G., Malinowski, B., Grześk, E., Wiciński, M., Szadujkis‑Szadurska, K."Direct regulation of vascular smooth muscle contraction by mastoparan‑7". Biomedical Reports 2.1 (2014): 34-38.
Chicago
Grześk, G., Malinowski, B., Grześk, E., Wiciński, M., Szadujkis‑Szadurska, K."Direct regulation of vascular smooth muscle contraction by mastoparan‑7". Biomedical Reports 2, no. 1 (2014): 34-38. https://doi.org/10.3892/br.2013.179
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team