Determination of imidocarb in animal biosamples using simple high‑performance liquid chromatography with diode-array detection
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- Published online on: October 29, 2013 https://doi.org/10.3892/br.2013.189
- Pages: 89-92
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Abstract
The aim of this study was to develop an analytical method for detection of imidocarb [1,3‑bis[3‑(4,5‑dihydro‑1h‑imidazol‑2‑yl)phenyl]urea] in beef and milk using high‑performance liquid chromatography (HPLC) with diode‑array detection (DAD). Imidocarb was separated on a reversed‑phase column (4.6x250 mm, 5 µm) with a mobile phase consisting of 85:15 (v/v) 0.1% trifluoroacetic acid/acetonitrile. The flow rate was 1 ml/min, and the column temperature was maintained at 20˚C. Detection was carried out at 260 nm using a DAD detector. The analytical samples were extracted using a solid‑phase extraction (SPE) method. The calibration curves showed good linearity (r≥0.998). Limits of quantifications (LOQs) were 0.15 mg/kg in beef and 0.025 mg/kg in milk. Intra‑ and inter‑day precisions were 3.2‑6.1 and 1.4‑6.9%, respectively, and the accuracy (recovery) was 80.4‑82.2% and 80.1‑89.5% in beef and milk, respectively. Thus, an analytical protocol using SPE extraction followed by HPLC with DAD was successfully developed, which demonstrated acceptable precision and recovery.
