Determination of imidocarb in animal biosamples using simple high‑performance liquid chromatography with diode-array detection

Park,Jin‑A.; Cho,Hee‑Jung; Cho,Soo‑Min; Kim,Na‑Hyun; Kim,Dong‑Soon; Zhang,Dan; Kim,Seong‑Kwan; Yi,Hee; Shin,Ho‑Chul

doi:10.3892/br.2013.189

January-February 2014 Volume 2 Issue 1

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January-February 2014 Volume 2 Issue 1

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Article

Determination of imidocarb in animal biosamples using simple high‑performance liquid chromatography with diode-array detection

Authors:
- Jin‑A. Park
- Hee‑Jung Cho
- Soo‑Min Cho
- Na‑Hyun Kim
- Dong‑Soon Kim
- Dan Zhang
- Seong‑Kwan Kim
- Hee Yi
- Ho‑Chul Shin
View Affiliations / Copyright

Affiliations: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143‑701, Republic of Korea
Pages: 89-92
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Published online on: October 29, 2013

https://doi.org/10.3892/br.2013.189
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Abstract

The aim of this study was to develop an analytical method for detection of imidocarb [1,3‑bis[3‑(4,5‑dihydro‑1h‑imidazol‑2‑yl)phenyl]urea] in beef and milk using high‑performance liquid chromatography (HPLC) with diode‑array detection (DAD). Imidocarb was separated on a reversed‑phase column (4.6x250 mm, 5 µm) with a mobile phase consisting of 85:15 (v/v) 0.1% trifluoroacetic acid/acetonitrile. The flow rate was 1 ml/min, and the column temperature was maintained at 20˚C. Detection was carried out at 260 nm using a DAD detector. The analytical samples were extracted using a solid‑phase extraction (SPE) method. The calibration curves showed good linearity (r≥0.998). Limits of quantifications (LOQs) were 0.15 mg/kg in beef and 0.025 mg/kg in milk. Intra‑ and inter‑day precisions were 3.2‑6.1 and 1.4‑6.9%, respectively, and the accuracy (recovery) was 80.4‑82.2% and 80.1‑89.5% in beef and milk, respectively. Thus, an analytical protocol using SPE extraction followed by HPLC with DAD was successfully developed, which demonstrated acceptable precision and recovery.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Determination of imidocarb in animal biosamples using simple high‑performance liquid chromatography with diode-array detection

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