CTCF and CTCFL mRNA expression in 17β-estradiol‑treated MCF7 cells

Del Campo,Eduardo   Portillo; Talamás Márquez,José  Jorge; Reyes-Vargas,Francianella; Del Pilar Intriago-Ortega,María; Quintanar-Escorza,Martha  Angélica; Burciaga-Nava,Jorge Alberto; Sifuentes-Alvarez,Antonio; Reyes-Romero,Miguel

doi:10.3892/br.2013.200

CTCF and CTCFL mRNA expression in 17β-estradiol‑treated MCF7 cells

Authors:
- Eduardo Portillo Del Campo
- José Jorge Talamás Márquez
- Francianella Reyes-Vargas
- María Del Pilar Intriago-Ortega
- Martha Angélica Quintanar-Escorza
- Jorge Alberto Burciaga-Nava
- Antonio Sifuentes-Alvarez
- Miguel Reyes-Romero
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Affiliations: Department of Molecular Medicine, Faculty of Medicine and Nutrition, Juárez University of the State of Durango, Durango, Durango 34000, Mexico , Department of Molecular Medicine, Faculty of Medicine and Nutrition, Juárez University of the State of Durango, Durango, Durango 34000, Mexico, Cancer State Center of the Ministry of Health, Durango, Durango 34000, Mexico, Department of Biochemistry, Faculty of Medicine and Nutrition, Juárez University of the State of Durango, Durango, Durango 34000, Mexico
Published online on: November 15, 2013 https://doi.org/10.3892/br.2013.200
Pages: 101-104

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Abstract

Estrogens play a key role in breast cancer, with 60-70% of the cases expressing estrogen receptors (ERs), which are encoded by the ESR1 gene. CTCFL, a paralogue of the chromatin organizer CTCF, is a potential biomarker of breast cancer, but its expression in this disease is currently controversial. A positive correlation has been reported between CTCFL and ERs in breast tumors and there also exists a coordinated interaction between CTCF and ERs in breast cancer cells. Therefore, there appears to be an association between CTCF, CTCFL and estrogens in breast cancer; however, there has been no report on the effects of estrogens on CTCF and CTCFL expression. The aim of this study was to determine the effect of 17β-estradiol (E2) on the CTCF and CTCFL mRNA expression in the MCF7 breast cancer cell line. the promoter methylation status of CTCFL and data mining for estrogen response elements in promoters of the CTCF and CTCFL genes were also determined. The transcription of CTCF and CTCFL was performed by quantitative polymerase chain reaction (qPCR) and the promoter methylation status of CTCFL was determined by methylation‑specific PCR. The MCF7 cells exhibited basal transcription of CTCF, which was significantly downregulated to 0.68 by 1 µM E2; basal or E2‑regulated transcription of CTCFL was not detected. Under basal conditions, the CTCFL promoter was methylated. Through data mining, an estrogen response element was identified in the CTCF promoter, but no such element was found in CTCFL. These results suggested that estrogens may modulate CTCF expression, although there was no apparent association between ERs and CTCFL.

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