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Article

Effect of yessotoxin on cytosolic calcium levels in human hepatocellular carcinoma cells in vitro

  • Authors:
    • Min Pang
    • Pei Qu
    • Chun-Lei Gao
    • Xuexi Tang
    • Zong-Ling Wang
  • View Affiliations / Copyright

    Affiliations: First Institute of Oceanography, State Oceanic Administration, Qingdao, Shandong 266061, P.R. China, College of Marine Life Science, Ocean University of China, Qingdao, Shandong 266100, P.R. China
  • Pages: 93-96
    |
    Published online on: November 19, 2013
       https://doi.org/10.3892/br.2013.202
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Abstract

Yessotoxin (YTX) and its analogs are a type of marine toxins found in marine environments in numerous coastal countries. These toxins tend to accumulate in filter‑feeding molluscs and may threaten the shellfish industry and public health. Several previous studies indicated that YTX may induce apoptosis in different types of cell lines, although the exact underlying mechanisms have not yet been elucidated. The aim of this study was to mainly focus on the effect of YTX on cytosolic Ca2+ levels in human hepatocellular carcinoma cells. in order to investigate the exact mechanism of YTX-evoked Ca2+ increase, laser scanning confocal microscopy was used, with the addition of the chelator ethylene glycol tetraacetic acid (EGTA) and nifedipine, an L-type Ca2+ channel blocker, to the reaction system. The results demonstrated that YTX caused cytosolic Ca2+ level increase in Bel7402 cells and the YTX-evoked Ca2+ increase was successfully blocked by EGTA and nifedipine. Therefore, our results indicated that YTX may cause apoptosis via inducing Ca2+ entry in Bel7402 cells.
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1 

Satake M, MacKenzie L and Yasumoto T: Identification of Protoceratium reticulatum as the biogenetic origin of yessotoxin. Nat Toxins. 5:164–167. 1997.

2 

Draisci R, Ferretti E, Palleschi L, et al: High levels of yessotoxin in mussels and presence of yessotoxin and homoyessotoxin in dinoflagellates of the Adriatic Sea. Toxicon. 37:1187–1193. 1999. View Article : Google Scholar : PubMed/NCBI

3 

Stobo LA, Lewis J, Quilliam MA, et al: Detection of yessotoxin in UK and Canadian isolates of phytoplankton and optimization and validation of LC-MS methods. Proceedings of the Eighth Canadian Workshop on Harmful Marine Algae; Bates S: Can Tech Rep Fish Aquat Sci. Gulf Fisheries Centre; Moncton: pp. 8–14. 2003

4 

Ciminiello P, Dell’Aversano C, Fattorusso E, et al: Complex yessotoxins profile in Protoceratium reticulatum from northwestern Adriatic sea revealed by LC-MS analysis. Toxicon. 42:7–14. 2003.(In Chinese).

5 

Arévalo F, Pazos Y, Correa J, Salgado C, Moroño A, Paz B and Franco JM: First report of yessotoxins in mussels of Galician Rías during a bloom of Lingulodinium polyedra Stein (Dodge). Henshilwood K, Deegan B, McMahon T, Cusack C, Keaveney S, Silke J, O’Cinneide M, Lyons D and Hess P: Galway. pp. 184–189. 2006

6 

Morton SL, Vershinin A, Leighfield TA, Smith L and Quilliam M: Identification of yessotoxin in mussels from the Caucasian Black Sea Coast of the Russian Federation. Toxicon. 50:581–584. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Gao CL, Liu RY, Liang YB, et al: First report of the presence of yessotoxins (YTXs) in shellfish from China’s coastal areas. Acta Oceanol Sin. 3:129–137. 2010.PubMed/NCBI

8 

Murata M, Masanori K, Lee JS and Yasumoto T: Isolation and structure of yessotoxin, a novel polyether compound implicated in diarrhetic shellfish poisoning. Tetrahedron Lett. 28:5869–5872. 1987. View Article : Google Scholar

9 

Yasumoto T and Takizawa A: Fluorometric measurement of yessotoxins in shellfish by high-pressure liquid chromatography. Biosci Biotechnol Biochem. 61:1775–1777. 1997. View Article : Google Scholar : PubMed/NCBI

10 

2002/225/EC. Commission Decision of 15 March 2002 laying down detailed rules for the implementation of Council Directive 91/492/EEC as regards the maximum levels and the methods of analysis of certain marine biotoxins in bivalve molluscs, echinoderms, tunicates and marine gastropods. Official Journal of the European Communities. 622002.

11 

Green DR and Reed JC: Mitochondria and apoptosis. Science. 281:1309–1312. 1998. View Article : Google Scholar

12 

Green DR and Evan GI: A matter of life and death. Cancer Cell. 1:19–30. 2002. View Article : Google Scholar

13 

Green DR and Kroemer G: The pathophysiology of mitochondrial cell death. Science. 305:626–629. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Malagutia C, Ciminiellob P, Fattorussob E and Rossinia GP: Caspase activation and death induced by yessotoxin in HeLa cells. Toxicol In Vitro. 16:357–363. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Leira F, Alvarez C, Vieites JM, Vieytes MR and Botana LM: Characterization of distinct apoptotic changes induced by okadaic acid and yessotoxin in the BE(2)-M17 neuroblastoma cell line. Toxicol In Vitro. 16:23–31. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Suárez Korsnes M, Hetland DL, Espenes A and Aune T: Induction of apoptosis by YTX in myoblast cell lines via mitochondrial signalling transduction pathway. Toxicol In Vitro. 20:1419–1426. 2006.PubMed/NCBI

17 

Suárez Korsnes M, Hetland DL, Espenes A, Tranulis MA and Aune T: Apoptotic events induced by yessotoxin in myoblast cell lines from rat and mouse. Toxicol In Vitro. 20:1077–1087. 2006.PubMed/NCBI

18 

Suárez Korsnes M, Hetland DL, Espenes A and Aune T: Cleavage of tensin during cytoskeleton disruption in YTX-induced apoptosis. Toxicol In Vitro. 21:9–15. 2007.PubMed/NCBI

19 

Pang M, Gao CL, Wu ZX, Lv N, Wang ZL, Tang XX and Qu P: Apoptosis induced by yessotoxins in HeLa human cervical cancer cells in vitro. Mol Med Rep. 3:629–634. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Pang M, Wang ZL, Gao CL, Qu P and Li HD: Characterization of apoptotic changes induced by yessotoxin in the Bel7402 human hepatoma cell line. Mol Med Rep. 4:547–552. 2011.PubMed/NCBI

21 

Pang M, Qu P, Gao CL and Wang ZL: Yessotoxin induces apoptosis in HL7702 human liver cells. Mol Med Rep. 5:211–216. 2012.PubMed/NCBI

22 

de la Rosa LA, Alfonso A, Vilarino N, Vieytes MR and Botana LM: Modulation of cytosolic calcium levels of human lymphocytes by yessotoxin, a novel marine phycotoxin. Biochem Pharmacol. 61:827–833. 2001.PubMed/NCBI

23 

Perez-Gomez A, Fererro-Gutierrez A, Novelli A, Franco JM, Paz B and Fernandez-Sanchez MT: Potent neurotoxic action of the shellfish biotoxin yessotoxin on cultured cerebellar neurons. Toxicol Sci. 90:168–177. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Korsnes MS, Hetland DL, Espenes A and Aune T: Induction of apoptosis by YTX in myoblast cell lines via mitochondrial signalling transduction pathway. Toxicol In Vitro. 20:1419–1426. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Korsnes MS, Hetland DL, Espenes A, Tranulis MA and Aune T: Apoptotic events induced by yessotoxin in myoblast cell lines from rat and mouse. Toxicol In Vitro. 20:1077–1087. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Malagoli D, Marchesini E and Ottaviani E: Lysosomes as the target of yessotoxin in invertebrate and vertebrate cell lines. Toxicol Lett. 167:75–83. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Botana Lopez LM, Alfonso Rancano A, Rodriguez Vieytes M and Loza Garcia MI: Therapeutic use of yessotoxins as human tumor cell growth inhibitors EP 1875906 B1. Filed July 21, 2004; issued June 29, 2011.

28 

Martin SJ, Reutelingsperger CP, McGahon AJ, Rader JA, van Schie RC, LaFace DM and Green DR: Early redistribution of plasma membrane phosphatidylserines is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J Exp Med. 182:1545–1556. 1995. View Article : Google Scholar

29 

Popov SG, Villasmil R, Bernardi J, et al: Lethal toxin of Bacillus anthracis causes apoptosis of macrophages. Biochem Bioph Res Commun. 293:349–355. 2002.PubMed/NCBI

30 

Berridge MJ, Lipp P and Bootman MD: The versatility and universality of calcium signalling. Nat Rev Mol Cell Biol. 1:11–21. 2000. View Article : Google Scholar : PubMed/NCBI

31 

Mattson MP and Chan SL: Calcium orchestrates apoptosis. Nat Cell Biol. 5:1041–1043. 2003. View Article : Google Scholar

32 

Ferrari D, Pinton P, Szabadkai G, Chami M, Campanella M, Pozzan T and Rizzuto R: Endoplasmic reticulum, Bcl-2 and Ca2+handling in apoptosis. Cell Calcium. 32:413–420. 2002. View Article : Google Scholar : PubMed/NCBI

33 

Smaili SS, Hsu YT, Carvalho ACP, Rosenstock TR, Sharpe JC and Youle RJ: Mitochondria, calcium and pro-apoptotic proteins as mediators in cell death signaling. Braz J Med Biol Res. 36:183–190. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Pang M, Qu P, Gao CL, Wang ZL and Zhang Y: Apoptosis and intracellular calcium level change induced by yessotoxin in HeLa cells. J Fish China. 35:1729–1735. 2011.

35 

Jiang S, Chow SC, Nicotera P and Orrenius S: Intracellular Ca2+signals activate apoptosis in thymocytes: studies using the Ca2+-ATPase inhibitor thapsigargin. Exp Cell Res. 212:84–92. 1994.

36 

Wertz IE and Dixit VM: Characterization of calcium release-activated apoptosis of LNCaP prostate cancer cells. J Biol Chem. 275:11470–11477. 2000. View Article : Google Scholar : PubMed/NCBI

37 

Pinton P, Ferrari D, Rapizzi E, Di Virgilio F, Pozzan T and Rizzuto R: The Ca2+concentration of the endoplasmic reticulum is a key determinant of ceramide-induced apoptosis: significance for the molecular mechanism of Bcl-2 action. EMBO J. 20:2690–2701. 2001.

38 

Putney JW Jr: Receptor-regulated calcium entry. Pharmacol Ther. 48:427–434. 1990. View Article : Google Scholar

39 

Putney JW Jr: Capacitative calcium entry revisited: Review article. Cell Calcium. 11:611–624. 1990. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Pang M, Qu P, Gao C, Tang X and Wang Z: Effect of yessotoxin on cytosolic calcium levels in human hepatocellular carcinoma cells in vitro. Biomed Rep 2: 93-96, 2014.
APA
Pang, M., Qu, P., Gao, C., Tang, X., & Wang, Z. (2014). Effect of yessotoxin on cytosolic calcium levels in human hepatocellular carcinoma cells in vitro. Biomedical Reports, 2, 93-96. https://doi.org/10.3892/br.2013.202
MLA
Pang, M., Qu, P., Gao, C., Tang, X., Wang, Z."Effect of yessotoxin on cytosolic calcium levels in human hepatocellular carcinoma cells in vitro". Biomedical Reports 2.1 (2014): 93-96.
Chicago
Pang, M., Qu, P., Gao, C., Tang, X., Wang, Z."Effect of yessotoxin on cytosolic calcium levels in human hepatocellular carcinoma cells in vitro". Biomedical Reports 2, no. 1 (2014): 93-96. https://doi.org/10.3892/br.2013.202
Copy and paste a formatted citation
x
Spandidos Publications style
Pang M, Qu P, Gao C, Tang X and Wang Z: Effect of yessotoxin on cytosolic calcium levels in human hepatocellular carcinoma cells in vitro. Biomed Rep 2: 93-96, 2014.
APA
Pang, M., Qu, P., Gao, C., Tang, X., & Wang, Z. (2014). Effect of yessotoxin on cytosolic calcium levels in human hepatocellular carcinoma cells in vitro. Biomedical Reports, 2, 93-96. https://doi.org/10.3892/br.2013.202
MLA
Pang, M., Qu, P., Gao, C., Tang, X., Wang, Z."Effect of yessotoxin on cytosolic calcium levels in human hepatocellular carcinoma cells in vitro". Biomedical Reports 2.1 (2014): 93-96.
Chicago
Pang, M., Qu, P., Gao, C., Tang, X., Wang, Z."Effect of yessotoxin on cytosolic calcium levels in human hepatocellular carcinoma cells in vitro". Biomedical Reports 2, no. 1 (2014): 93-96. https://doi.org/10.3892/br.2013.202
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