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Article

Serotonin induces the migration of PC12 cells via the serotonin receptor 6/cAMP/ERK pathway

  • Authors:
    • Keita Koizumi
    • Hideo Nakajima
  • View Affiliations / Copyright

    Affiliations: Center for Child Mental Development, Kanazawa University, Kanazawa, Ishikawa, Japan, Department of Oncology, Ageo Central General Hospital, Ageo, Saitama, Japan
  • Pages: 29-33
    |
    Published online on: November 19, 2013
       https://doi.org/10.3892/br.2013.203
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Abstract

Serotonin (5-HT) functions as a chemoattractant that modulates neural migration during prenatal and early postnatal development. However, its molecular mechanism remains to be elucidated. The effect of 5-HT on neural cell migration was examined using PC12 neuron-like cell line. Transwell migration assay was used to determine the effect of 5-HT on PC12 cell migration. The results demonstrated that 5-HT and nerve growth factor (NGF) induced PC12 cell migration in a dose‑dependent manner. Additionally, 5-HT receptor antagonists suggest that 5-HT‑induced migration was mediated by serotonin receptor 6 (5-HT6), a Gs-protein coupled receptor that elevates the intercellular cAMP level. By contrast, antagonists of serotonin receptor 3 (5-HT3) did not show any effects on PC12 cell migration. Clozapine, an inhibitor of cAMP accumulation mediated by 5-HT6, significantly reduced the effect of 5-HT on the PC12 cell migration. An inhibitor of extracellular signal-regulated kinase (ERK) also suppressed migration. These results suggest that 5-HT induces PC12 cell migration by activating cAMP/ERK signaling pathways, which is mediated by 5-HT6 receptor.
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1 

Valiente M and Marín O: Neuronal migration mechanisms in development and disease. Curr Opin Neurobiol. 20:68–78. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Brennand KJ, Simone A, Tran N and Gage FH: Modeling psychiatric disorders at the cellular and network levels. Mol Psychiatry. 17:1239–1253. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Oliveira SL, Pillat MM, Cheffer A, et al: Functions of neurotrophins and growth factors in neurogenesis and brain repair. Cytometry A. 83:76–89. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Wang DD and Kriegstein AR: Defining the role of GABA in cortical development. J Physiol. 587:1873–1879. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Hernandez A, Kimball B, Romanchuk G and Mulholland MW: Pituitary adenylate cyclase-activating peptide stimulates neurite growth in PC12 cells. Peptides. 16:927–932. 1995. View Article : Google Scholar : PubMed/NCBI

6 

Bonnin A, Goeden N, Chen K, et al: A transient placental source of serotonin for the fetal forebrain. Nature. 472:347–350. 2011.PubMed/NCBI

7 

Vitalis T, Cases O, Passemard S, et al: Embryonic depletion of serotonin affects cortical development. Eur J Neurosci. 26:331–344. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Riccio O, Potter G, Walzer C, et al: Excess of serotonin affects embryonic interneuron migration through activation of the serotonin receptor 6. Mol Psychiatry. 14:280–290. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Riccio O, Jacobshagen M, Golding B, et al: Excess of serotonin affects neocortical pyramidal neuron migration. Transl Psychiatry. 1:e472011. View Article : Google Scholar : PubMed/NCBI

10 

Millan MJ, Marin P, Bockaert J, et al: Signaling at G-protein-coupled serotonin receptors: recent advances and future research directions. Trends Pharmacol Sci. 29:454–464. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Hannon J and Hoyer D: Molecular biology of 5-HT receptors. Behav Brain Res. 195:198–213. 2008. View Article : Google Scholar

12 

Lee S, Hjerling-Leffler J, Zagha E, et al: The largest group of superficial neocortical GABAergic interneurons expresses ionotropic serotonin receptors. J Neurosci. 30:16796–16808. 2010. View Article : Google Scholar

13 

Vitalis T, Ansorge MS and Dayer AG: Serotonin homeostasis and serotonin receptors as actors of cortical construction: special attention to the 5-HT3Aand 5-HT6receptor subtypes. Front Cell Neurosci. 7:932013. View Article : Google Scholar : PubMed/NCBI

14 

Hassoun AT, Erdélyi F, Szabó G and Davis MI: A rapid screening method for population-specific neuronal motogens, substrates and associated signaling pathways. J Neurosci Methods. 166:178–194. 2007. View Article : Google Scholar

15 

Ho W, Uniyal S, Meakin SO, et al: A differential role of extracellular signal-regulated kinase in stimulated PC12 pheochromocytoma cell movement. Exp Cell Res. 263:254–264. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Yoshizawa M, Kawauchi T, Sone M, et al: Involvement of a Rac activator, P-Rex1, in neurotrophin-derived signaling and neuronal migration. J Neurosci. 25:4406–4419. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Homma K, Kitamura Y, Ogawa H and Oka K: Serotonin induces the increase in intracellular Ca2+that enhances neurite outgrowth in PC12 cells via activation of 5-HT3receptors and voltage-gated calcium channels. J Neurosci Res. 84:316–325. 2006.PubMed/NCBI

18 

Choi YH, Kang H, Lee WK, et al: An inhibitory compound against the interaction between Gαsand the third intracellular loop region of serotonin receptor subtype 6 (5-HT6) disrupts the signaling pathway of 5-HT6. Exp Mol Med. 39:335–342. 2007.PubMed/NCBI

19 

Kohen R, Metcalf MA, Khan N, et al: Cloning, characterization, and chromosomal localization of a human 5-HT6serotonin receptor. J Neurochem. 66:47–56. 1996. View Article : Google Scholar : PubMed/NCBI

20 

Vossler MR, Yao H, York RD, et al: cAMP activates MAP kinase and Elk-1 through a B-Raf- and Rap1-dependent pathway. Cell. 89:73–82. 1997. View Article : Google Scholar : PubMed/NCBI

21 

Côté F, Fligny C, Bayard E, et al: Maternal serotonin is crucial for murine embryonic development. Proc Natl Acad Sci USA. 104:329–334. 2007.PubMed/NCBI

22 

Narboux-Nême N, Angenard G, Mosienko V, et al: Postnatal growth defects in mice with constitutive depletion of central serotonin. ACS Chem Neurosci. 4:171–181. 2013.PubMed/NCBI

23 

Cheng A, Scott AL, Ladenheim B, et al: Monoamine oxidases regulate telencephalic neural progenitors in late embryonic and early postnatal development. J Neurosci. 30:10752–10762. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Lieske V, Bennett-Clarke CA and Rhoades RW: Effects of serotonin on neurite outgrowth from thalamic neurons in vitro. Neuroscience. 90:967–974. 1999. View Article : Google Scholar : PubMed/NCBI

25 

Lotto B, Upton L, Price DJ and Gaspar P: Serotonin receptor activation enhances neurite outgrowth of thalamic neurones in rodents. Neurosci Lett. 269:87–90. 1999. View Article : Google Scholar : PubMed/NCBI

26 

Zachor DA, Moore JF, Brezausek C, et al: Cocaine inhibits NGF-induced PC12 cells differentiation through D1-type dopamine receptors. Brain Res. 869:85–97. 2000. View Article : Google Scholar : PubMed/NCBI

27 

Whelchel A, Evans J and Posada J: Inhibition of ERK activation attenuates endothelin-stimulated airway smooth muscle cell proliferation. Am J Respir Cell Mol Biol. 16:589–596. 1997. View Article : Google Scholar : PubMed/NCBI

28 

Vaudry D, Stork PJ, Lazarovici P and Eiden LE: Signaling pathways for PC12 cell differentiation: making the right connections. Science. 296:1648–1649. 2002. View Article : Google Scholar : PubMed/NCBI

29 

Lazarovici P, Jiang H and Fink D Jr: The 38-amino-acid form of pituitary adenylate cyclase-activating polypeptide induces neurite outgrowth in PC12 cells that is dependent on protein kinase C and extracellular signal-regulated kinase but not on protein kinase A, nerve growth factor receptor tyrosine kinase, p21rasG protein, and pp60c-srccytoplasmic tyrosine kinase. Mol Pharmacol. 54:547–558. 1998.

30 

Engel M, Smidt MP and van Hooft JA: The serotonin 5-HT3receptor: a novel neurodevelopmental target. Front Cell Neurosci. 7:762013.

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Copy and paste a formatted citation
Spandidos Publications style
Koizumi K and Nakajima H: Serotonin induces the migration of PC12 cells via the serotonin receptor 6/cAMP/ERK pathway. Biomed Rep 2: 29-33, 2014.
APA
Koizumi, K., & Nakajima, H. (2014). Serotonin induces the migration of PC12 cells via the serotonin receptor 6/cAMP/ERK pathway. Biomedical Reports, 2, 29-33. https://doi.org/10.3892/br.2013.203
MLA
Koizumi, K., Nakajima, H."Serotonin induces the migration of PC12 cells via the serotonin receptor 6/cAMP/ERK pathway". Biomedical Reports 2.1 (2014): 29-33.
Chicago
Koizumi, K., Nakajima, H."Serotonin induces the migration of PC12 cells via the serotonin receptor 6/cAMP/ERK pathway". Biomedical Reports 2, no. 1 (2014): 29-33. https://doi.org/10.3892/br.2013.203
Copy and paste a formatted citation
x
Spandidos Publications style
Koizumi K and Nakajima H: Serotonin induces the migration of PC12 cells via the serotonin receptor 6/cAMP/ERK pathway. Biomed Rep 2: 29-33, 2014.
APA
Koizumi, K., & Nakajima, H. (2014). Serotonin induces the migration of PC12 cells via the serotonin receptor 6/cAMP/ERK pathway. Biomedical Reports, 2, 29-33. https://doi.org/10.3892/br.2013.203
MLA
Koizumi, K., Nakajima, H."Serotonin induces the migration of PC12 cells via the serotonin receptor 6/cAMP/ERK pathway". Biomedical Reports 2.1 (2014): 29-33.
Chicago
Koizumi, K., Nakajima, H."Serotonin induces the migration of PC12 cells via the serotonin receptor 6/cAMP/ERK pathway". Biomedical Reports 2, no. 1 (2014): 29-33. https://doi.org/10.3892/br.2013.203
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