P‑selectin expression in a colon tumor model exposed by sinusoidal electromagnetic fields

Tuncel,Handan; Shimamoto,Fumio; Çırakoğlu,Ayşe; Korpınar,Mehmet  Ali; Kalkan,Tunaya

doi:10.3892/br.2013.81

P‑selectin expression in a colon tumor model exposed by sinusoidal electromagnetic fields

Authors:
- Handan Tuncel
- Fumio Shimamoto
- Ayşe Çırakoğlu
- Mehmet Ali Korpınar
- Tunaya Kalkan
View Affiliations

Affiliations: Department of Biophysics, Cerrahpaşa Medical Faculty, Istanbul University, Fatih, Istanbul 34303, Turkey, Prefectural University of Hiroshima, Hiroshima 727‑0023, Japan, Department of Medical Biology, Cerrahpaşa Medical Faculty, Istanbul University, Fatih, Istanbul 34303, Turkey
Published online on: March 14, 2013 https://doi.org/10.3892/br.2013.81
Pages: 389-392

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

P‑selectin is mainly involved in the initial process of tumor cell adhesion to platelets. The aim of the present study was to determine the expression level of P‑selectin in a colon tumor model affected by sinusoidal electromagnetic fields (SMF). Male Wistar albino rats aged 2‑2.5 months were used. The animals were divided into the I [N‑Methyl‑N‑Nitrosurea (MNU)], II (SMF‑MNU), III (SMF) and IV (control) groups. The rats were housed five per polycarbonate cage. Sixty milligrams of MNU was dissolved in 6 ml sterile 0.9% NaCl. Prepared solutions were administered intra rectally (i.r.) to the 1st and 3rd groups as 0.2 ml/per animal. The same procedure was applied to the 2nd and 4th groups, although 0.2 ml/per animal sterile isotonic solution was administered instead. This procedure was repeated once a week for 10 weeks. Following the administration of MNU, the 2nd and 3rd groups were exposed to a sinusoidal magnetic field (SMF, 50 Hz, 5 mT) for 6 h/day for 8 months. P‑selectin expression of the four groups of rat colon tissues was determined using immunohistochemistry on paraffin sections. The labeled streptavidin biotin method was performed. Fisher's exact test was used for differences between proportions. Results showed that there was no statistically significant (P>0.05) change in the expression level of P‑selectin. However, this result should be verified by both in vivo and in vitro experiments to determine the effects of the magnetic fields on P-selectin.

View Figures

View References

1	Giacomoni PU and Rein G: Factors of skin ageing share common mechanisms. Biogerontology. 2:219–229. 2001. View Article : Google Scholar : PubMed/NCBI
2	Tofani S, Barone D, Cintorino M, et al: Static and ELF magnetic fields induce tumor growth inhibition and apoptosis. Bioelectromagnetics. 22:419–428. 2001. View Article : Google Scholar : PubMed/NCBI
3	Jiménez-García MN, Arellanes-Robledo J, Aparicio-Bautista DI, Rodríguez-Segura MA, Villa-Treviño S and Godina-Nava JJ: Anti-proliferative effect of extremely low frequency electromagnetic field on preneoplastic lesions formation in the rat liver. BMC Cancer. 10:1592010.PubMed/NCBI
4	Merola P, Marino C, Lovisolo GA, Pinto R, Laconi C and Negroni A: Proliferation and apoptosis in a neuroblastoma cell line exposed to 900 MHz modulated radiofrequency field. Bioelectromagnetics. 27:164–171. 2006. View Article : Google Scholar : PubMed/NCBI
5	Santini MT, Rainaldi G, Ferrante A, Indovina PL, Vecchia P and Donelli G: Effects of a 50 Hz sinusoidal magnetic field on cell adhesion molecule expression in two human osteosarcoma cell lines (MG-63 and Saos-2). Bioelectromagnetics. 24:327–338. 2003. View Article : Google Scholar
6	Chen M and Geng JG: P-selectin mediated adhesion of leukocytes, platelets, and cancer cells in inflammation, trombosis, and cancer growth and metastasis. Arch Immunol Ther Exp. 54:75–84. 2006. View Article : Google Scholar
7	Läubli H and Borsig L: Selectins promote tumor metastasis. Semin Cancer Biol. 20:169–177. 2010.PubMed/NCBI
8	Miller FJ and Schroeer D: College Physics. 6th edition. Harcourt Brace; Jananovich, San Diego: 1987
9	Theriault G, Goldberg M, Miller AB, et al: Cancer risks associated with occupational exposure to magnetic fieldsamong electric utility workers in Ontario and Quebec, Canada, and France: 1970–1989. Am J Epidemiol. 139:550–572. 1994.
10	Simko M and Mattsson M-O: Extremely low frequency electromagnetic fields as effectors of cellular responses in vitro: possible immune cell activation. J Cell Biochem. 93:83–92. 2004. View Article : Google Scholar : PubMed/NCBI
11	Huang L, Dong L, Chen Y, Qi H and Xiao D: Effects of sinusoidal 50 Hz magnetic field on viability, cell cycle and apoptosis of HL-60 cells. Eur Phys J Appl Phys. 35:217–221. 2006. View Article : Google Scholar
12	Berg H, Günther B, Hilger I, Radeva M, Traitcheva N and Wollweber L: Bioelectromagnetic field effects on cancer cells and mice tumors. Electromagn Biol Med. 29:132–143. 2010. View Article : Google Scholar : PubMed/NCBI
13	Barbault A, Costa FP, Bottger B, Munden RF, Bomholt F, Kuster N and Pasche B: Amplitude-modulated electromagnetic fields for the treatment of cancer: discovery of tumor-specific frequencies and assessment of a novel therapeutic approach. J Exp Clin Cancer Res. 28:512009. View Article : Google Scholar
14	Albelda SM and Buck CA: Integrins and other cell adhesion molecules. FASEB J. 4:2868–2880. 1990.PubMed/NCBI
15	Miyasaka M: Cancer metastasis and adhesion molecules. Clin Orthop Relat Res. 312:10–18. 1995.
16	Huang YW, Baluna R and Vitetta ES: Adhesion molecules as targets for cancer therapy. Histol Histopathol. 12:467–477. 1997.PubMed/NCBI
17	Gong L, Cai Y, Zhou X and Yang H: Activated platelets interact with lung cancer cells through P-selectin glycoprotein ligand-1. Pathol Oncol Res. 18:989–996. 2012. View Article : Google Scholar : PubMed/NCBI
18	Gong L, Mi HJ, Zhu H, Zhou X and Yang H: P-selectin-mediated platelet activation promotes adhesion of non-small cell lung carcinoma cells on vascular endothelial cells under flow. Mol Med Rep. 5:935–942. 2012.
19	Aruffo A, Dietsch MT, Wan H, Hellstrom KE and Hellstrom I: Granule membrane protein 140 (GMP 140) binds to carcinomas and carcinoma-derived cell lines. Proc Natl Acad Sci USA. 89:2292–2296. 1992. View Article : Google Scholar : PubMed/NCBI
20	Mannori G, Crottet P, Cecconi O, et al: Differential colon cancer cell adhesion to E-, P- and L-selectin: role of mucin type glyco-proteins. Cancer Res. 55:4425–4431. 1995.
21	Hahne M, Jager U, Isenmann S, Hallmann R and Vestweber D: Five TNF-inducible cell adhesion mechanism on the surface of mouse endothelioma cells mediate the binding of leukocytes. J Cell Biol. 121:655–664. 1993. View Article : Google Scholar
22	Sanders WE, Wilson RW, Ballantyne CM and Beaudet AL: Molecular cloning and analysis of in vivo expression of murine P-selectin. Blood. 80:795–800. 1992.PubMed/NCBI
23	Yao L, Pan J, Setiadi H, Patel KD and McEver RP: Interleukin 4 or oncostatin M induces a prolonged increase in P-selectin mRNA and protein in human cells. J Exp Med. 184:81–92. 1996. View Article : Google Scholar : PubMed/NCBI
24	Zhang D, Pan X, Ohno S, Osuga T, Sawada S and Sato K: No effects of pulsed electromagnetic fields on expression of cell adhesion molecules (integrin, CD44) and matrix metalloproteinase-2/9 in osteosarcoma cell lines. Bioelectromagnetics. 32:463–473. 2011. View Article : Google Scholar