Expression levels of microRNA-375 in pancreatic cancer

Song,Shiduo; Zhou,Jian; He,Songbing; Zhu,Dongming; Zhang,Zixiang; Zhao,Hua; Wang,Yi; Li,Dechun

doi:10.3892/br.2013.88

May-June 2013 Volume 1 Issue 3

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May-June 2013 Volume 1 Issue 3

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Article

Expression levels of microRNA-375 in pancreatic cancer

Authors:
- Shiduo Song
- Jian Zhou
- Songbing He
- Dongming Zhu
- Zixiang Zhang
- Hua Zhao
- Yi Wang
- Dechun Li
View Affiliations / Copyright

Affiliations: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
Pages: 393-398
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Published online on: March 27, 2013

https://doi.org/10.3892/br.2013.88
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Abstract

MicroRNAs (miRNAs) are small, non‑coding RNAs of endogenous origin that have been increasingly shown to have altered expressions in various cancer types. The expression levels of miR-375 have not been comprehensively investigated in pancreatic cancer. In this study, total RNA was extracted from 44 pairs of pancreatic cancer tissues and non‑tumor adjacent tissues, as well as from four pancreatic cancer cell lines, Panc-1, SW1990, BxpC3 and Patu8988. Following polyadenylation and reverse transcription, the expression levels of miR-375 were determined by real-time PCR and the difference in expression was calculated using the 2-∆∆Ct method. The correlation between the expression levels of miR‑375 and clinicopathological characteristics of pancreatic cancer was also assessed. miR-375 expression was frequently downregulated in the pancreatic cancer tissues compared to their non‑tumor counterparts (P<0.05; paired t-test). Moreover, a significantly low expression of miR-375 was found in the pancreatic cancer cell lines (Panc-1, P=0.016; SW1990, P=0.016; BxPC3, P=0.018; Patu8988, P=0.017; paired t-test). However, no significant correlations were observed between the low expression of miR-375 and parameters including gender, age, tumor size, tumor location and histological grade (P>0.05). The low expression of miR‑375 was correlated with pT stage, lymph node metastases and pTNM stage (P<0.05) (non‑parametric test; Mann-Whitney U test between 2 groups and Kruskal‑Wallis H test for ≥3 groups). In conclusion, miR-375 is potentially involved in the carcinogenesis of pancreatic cancers and serves as is a potential biomarker for pancreatic cancer.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Expression levels of microRNA-375 in pancreatic cancer

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