Antibiotic susceptibility and genotype patterns of Pseudomonas aeruginosa from mechanical ventilation-associated pneumonia in intensive care units

Xiao,Hui; Ye,Xiong; Liu,Qingzhong; Li,Li

doi:10.3892/br.2013.94

July-August 2013 Volume 1 Issue 4

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

July-August 2013 Volume 1 Issue 4

Full Size Image

Article

Antibiotic susceptibility and genotype patterns of Pseudomonas aeruginosa from mechanical ventilation-associated pneumonia in intensive care units

Authors:
- Hui Xiao
- Xiong Ye
- Qingzhong Liu
- Li Li
View Affiliations / Copyright

Affiliations: Department of Laboratory Medicine, Shanghai First People's Hospital, Medical College, Shanghai Jiao Tong University, Shanghai 200080, P.R. China, Department of Respiratory Medicine, PuDong Hospital, Shanghai 200319, P.R. China
Pages: 589-593
|
Published online on: April 16, 2013

https://doi.org/10.3892/br.2013.94
Expand metrics +

Abstract

Pseudomonas aeruginosa (P. aeruginosa) is a leading cause of morbidity and mortality in patients with ventilation‑associated pneumonia (VAP). It is difficult to treat this infection due to acquired resistance to various antibiotics. In order to understand the potential route of transmission, it is important to have detailed knowledge of the genotypes and antibiotic susceptibility of P. aeruginosa. The aim of this study was to determine antibiotic susceptibility using the broth microdilution minimum inhibitory concentration (MIC) method and to apply the Randomly Amplified Polymorphic DNA (RAPD) typing method for VAP caused by P. aeruginosa in 16 patients (8 men and 8 women; average age at inclusion, 67.8 years; range, 53-76 years). To determine antibiotic susceptibility, imipenem (IPM), cefepime (FEP) and meropenem (MEM) were administered for the treatment of P. aeruginosa, yielding an effectiveness of 75, 62.5, and 62.5%, respectively. According to the National Committee for Clinical Laboratory Standards (NCCLS) breakpoints, 8 (50%) of the 16 mechanical ventilation (MV) isolates were resistant to ceftazidime (CAZ) and pipenacillin (PIP). Amikacin (AK) and aztreonam (AZT) were not as effective against P. aeruginosa (75%). In addition, P. aeruginosa was completely resistant to ciprofloxacin (CIP). The MV isolates were susceptible to polymyxin B (PB). RAPD analysis revealed 12 genotypes for all the isolated P. aeruginosa, separated into 4 patterns. The results demonstrated a high incidence of P. aeruginosa isolated from VAP, with endogenous and cross infections being potential reasons for P. aeruginosa isolated from VAP in the intensive care units.

View Figures

View References

1	Fricks-Lima J, Hendrickson CM, Allgaier M, et al: Differences in biofilm formation and antimicrobial resistance of Pseudomonas aeruginosa isolated from airways of mechanically ventilated patients and cystic fibrosis patients. Int J Antimicrob Agents. 37:309–315. 2011.PubMed/NCBI
2	Garnacho-Montero J, Sa-Borges M, Sole-Violan J, et al: Optimal management therapy for Pseudomonas aeruginosa ventilator-associated pneumonia: an observational, multicenter study comparing monotherapy with combination antibiotic therapy. Critical Care Med. 35:1888–1895. 2007.PubMed/NCBI
3	Sevillano E, Valderrey C, Canduela MJ, Umaran A, Calvo F and Gallego L: Resistance to antibiotics in clinical isolates of Pseudomonas aeruginosa. Pathol Biol (Paris). 54:493–497. 2006.PubMed/NCBI
4	Libisch B, Watine J, Balogh B, et al: Molecular typing indicates an important role for two international clonal complexes in dissemination of VIM-producing Pseudomonas aeruginosa clinical isolates in Hungary. Res Microbiol. 159:162–168. 2008. View Article : Google Scholar : PubMed/NCBI
5	Bradbury R, Champion A and Reid DW: Poor clinical outcomes associated with a multi-drug resistant clonal strain of Pseudomonas aeruginosa in the Tasmanian cystic fibrosis population. Respirology. 13:886–892. 2008. View Article : Google Scholar : PubMed/NCBI
6	Mahenthiralingam E, Campbell ME, Foster J, Lam JS and Speert DP: Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis. J Clin Microbiol. 34:1129–1135. 1996.
7	Crnich J, Safdar N and Maki DG: The role of the intensive care unit environment in the pathogenesis and prevention of ventilator-associated pneumonia. Respir Care. 50:813–838. 2005.PubMed/NCBI
8	Gil-Perotin S, Ramirez P, Marti V, et al: Implications of endotracheal tube biofilm in ventilator-associated pneumonia response: a state of concept. Crit Care. 16:R932012. View Article : Google Scholar : PubMed/NCBI
9	Trouillet JL: Ventilator-associated pneumonia: a comprehensive review. Hosp Pract (Minneap). 40:165–175. 2012. View Article : Google Scholar : PubMed/NCBI
10	Farah R and Makhoul N: Optimal time needed for withdrawal of mechanical ventilation in patients with chronic obstructive pulmonary disease. Isr Med Assoc J. 13:609–612. 2011.PubMed/NCBI
11	Arnold HM, Sawyer AM and Kollef MH: Use of adjunctive aerosolized antimicrobial therapy in the treatment of Pseudomonas aeruginosa and Acinetobacter baumannii ventilator-associated pneumonia. Respir Care. 57:1226–1233. 2012. View Article : Google Scholar : PubMed/NCBI
12	Bassetti M, Taramasso L, Giacobbe DR and Pelosi P: Management of ventilator-associated pneumonia: epidemiology, diagnosis and antimicrobial therapy. Expert Rev Anti Infect Ther. 10:585–596. 2012. View Article : Google Scholar : PubMed/NCBI
13	Mahida K and Kwon DH: Co-existence of multidrug-resistant and -susceptible strains of Pseudomonas aeruginosa from a single clinical isolate. Curr Microbiol. 61:19–24. 2010. View Article : Google Scholar : PubMed/NCBI
14	Ruiz L, Dominguez MA, Ruiz N and Vinas M: Relationship between clinical and environmental isolates of Pseudomonas aeruginosa in a hospital setting. Arch Med Res. 35:251–257. 2004. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Antibiotic susceptibility and genotype patterns of Pseudomonas aeruginosa from mechanical ventilation-associated pneumonia in intensive care units

This article is mentioned in:

Abstract

Figure 1

Related Articles