Association of adiponectin +276G/T polymorphism with knee osteoarthritis

Zhan,Dong; Yuktanandana,Pongsak; Anomasiri,Wilai; Tanavalee,Aree; Honsawek,Sittisak

doi:10.3892/br.2014.228

Association of adiponectin +276G/T polymorphism with knee osteoarthritis

Authors:
- Dong Zhan
- Pongsak Yuktanandana
- Wilai Anomasiri
- Aree Tanavalee
- Sittisak Honsawek
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Affiliations: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand, Department of Orthopaedics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
Published online on: January 20, 2014 https://doi.org/10.3892/br.2014.228
Pages: 229-232

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Abstract

Osteoarthritis (OA) is a prevalent, degenerative joint disorder leading to the destruction of articular cartilage, osteophyte formation and subchondral bone sclerosis. Genetic and environmental factors are involved in the development of OA. The role of adiponectin gene polymorphisms in OA has not yet been established. The aim of this study was to investigate the association of adiponectin +276G/T (rs1501299) gene polymorphism with knee OA. Genotype distributions and allelic frequencies of adiponectin gene, +276G/T polymorphism were determined in a total of 200 subjects (100 knee OA patients and 100 healthy controls). Single‑nucleotide polymorphism (SNP) of the adiponectin +276G/T gene was genotyped by polymerase chain reaction (PCR)‑restriction fragment length polymorphism analysis. The genotype distribution of +276G/T SNP was observed in the Hardy‑Weinberg equilibrium for OA patients and controls. No statistically significant difference was identified between the two groups with respect to genotype distributions and allelic frequencies (P>0.05). The T‑ and G‑allele frequencies were indicated as 24.5 and 75.5%, respectively, in OA patients, whereas the frequency was 23‑70% in the control group. Findings of this study therefore suggest that the +276G/T SNP was not associated with susceptibility to knee OA.

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1	Krasnokutsky S, Attur M, Palmer G, et al: Current concepts in the pathogenesis of osteoarthritis. Osteoarthritis Cartilage. 16(Suppl 3): S1–S3. 2008. View Article : Google Scholar
2	Banks SE: Erosive osteoarthritis: a current review of a clinical challenge. Clin Rheumatol. 29:697–706. 2010. View Article : Google Scholar : PubMed/NCBI
3	Loeser RF: Aging and osteoarthritis: the role of chondrocyte senescence and aging changes in the cartilage matrix. Osteoarthritis Cartilage. 17:971–979. 2009. View Article : Google Scholar : PubMed/NCBI
4	Steinmeyer J: Cytokines in osteoarthritis - current status on the pharmacological intervention. Front Biosci. 9:575–580. 2004. View Article : Google Scholar : PubMed/NCBI
5	Hao SW and Jin QH: Association between the +104T/C polymorphism in the 5′UTR of GDF5 and susceptibility to knee osteoarthritis: A meta-analysis. Mol Med Rep. 7:485–488. 2013.
6	Bergink AP, van Meurs JB, Loughlin J, et al: Estrogen receptor alpha gene haplotype is associated with radiographic osteoarthritis of the knee in elderly men and women. Arthritis Rheum. 48:1913–1922. 2003. View Article : Google Scholar : PubMed/NCBI
7	Magana JJ, Galvez-Rosas A, Gonzalez-Huerta C, et al: Association of the calcitonin gene (CA) polymorphism with osteoarthritis of the knee in a Mexican mestizo population. Knee. 17:157–160. 2010. View Article : Google Scholar : PubMed/NCBI
8	Honsawek S, Deepaisarnsakul B, Tanavalee A, et al: Association of IL-6 −174G/C gene polymorphism with knee osteoarthritis in a Thai population. Genet Mol Res. 10:1674–1680. 2011.
9	Valdes AM, Spector TD, Tamm A, et al: Genetic variation in the SMAD3 gene is associated with hip and knee osteoarthritis. Arthritis Rheum. 62:2347–2352. 2010. View Article : Google Scholar : PubMed/NCBI
10	Honsawek S, Malila S, Yuktanandana P, et al: Association of MMP-3 (−1612 5A/6A) polymorphism with knee osteoarthritis in Thai population. Rheumatol Int. 33:435–439. 2013.
11	Waki H, Yamauchi T, Kamon J, et al: Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin. J Biol Chem. 278:40352–40363. 2003. View Article : Google Scholar : PubMed/NCBI
12	Kotani K, Sakane N, Saiga K, Sano Y and Kurozawa Y: Lifestyle-related determinants and serum adiponectin concentrations in a general population of Japanese females. Arch Med Res. 38:887–890. 2007. View Article : Google Scholar : PubMed/NCBI
13	Kellgren JH and Lawrence JS: Radiological assessment of osteo-arthrosis. Ann Rheum Dis. 16:494–502. 1957. View Article : Google Scholar : PubMed/NCBI
14	Nakatani K, Noma K, Nishioka J, et al: Adiponectin gene variation associates with the increasing risk of type 2 diabetes in non-diabetic Japanese subjects. Int J Mol Med. 15:173–177. 2005.PubMed/NCBI
15	Senolt L, Pavelka K, Housa D and Haluzik M: Increased adiponectin is negatively linked to the local inflammatory process in patients with rheumatoid arthritis. Cytokine. 35:247–252. 2006. View Article : Google Scholar : PubMed/NCBI
16	Toussirot E, Streit G and Wendling D: The contribution of adipose tissue and adipokines to inflammation in joint diseases. Curr Med Chem. 14:1095–1100. 2007. View Article : Google Scholar : PubMed/NCBI
17	Wulster-Radcliffe MC, Ajuwon KM, Wang J, Christian JA and Spurlock ME: Adiponectin differentially regulates cytokines in porcine macrophages. Biochem Biophys Res Commun. 316:924–929. 2004. View Article : Google Scholar : PubMed/NCBI
18	Wolf AM, Wolf D, Rumpold H, Enrich B and Tilg H: Adiponectin induces the anti-inflammatory cytokines IL-10 and IL-1RA in human leukocytes. Biochem Biophys Res Commun. 323:630–635. 2004. View Article : Google Scholar : PubMed/NCBI
19	Fantuzzi G: Adiponectin and inflammation: consensus and controversy. J Allergy Clin Immunol. 121:326–330. 2008. View Article : Google Scholar : PubMed/NCBI
20	Yamauchi T, Kamon J, Minokoshi Y, et al: Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase. Nat Med. 8:1288–1295. 2002. View Article : Google Scholar : PubMed/NCBI
21	von Eynatten M, Hamann A, Twardella D, et al: Relationship of adiponectin with markers of systemic inflammation, atherogenic dyslipidemia, and heart failure in patients with coronary heart disease. Clin Chem. 52:853–859. 2006.PubMed/NCBI
22	Lago R, Gomez R, Otero M, et al: A new player in cartilage homeostasis: adiponectin induces nitric oxide synthase type II and pro-inflammatory cytokines in chondrocytes. Osteoarthritis Cartilage. 16:1101–1109. 2008. View Article : Google Scholar : PubMed/NCBI
23	Choi HM, Lee YA, Lee SH, et al: Adiponectin may contribute to synovitis and joint destruction in rheumatoid arthritis by stimulating vascular endothelial growth factor, matrix metalloproteinase-1, and matrix metalloproteinase-13 expression in fibroblast-like synoviocytes more than proinflammatory mediators. Arthritis Res Ther. 11:R1612009.
24	Chen TH, Chen L, Hsieh MS, Chang CP, Chou DT and Tsai SH: Evidence for a protective role for adiponectin in osteoarthritis. Biochim Biophys Acta. 1762:711–718. 2006. View Article : Google Scholar : PubMed/NCBI