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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

CpG‑oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll‑like receptor 9 signaling and upregulation of Runt‑related transcription factor 3 expression

  • Authors:
    • Prince Amoah Barnie
    • Pan Zhang
    • Ping Lu
    • Xiaobo Chen
    • Zhaoliang Su
    • Shengjun Wang
    • Huaxi Xu
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China, Division of Nephrology, Department of Internal Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China
  • Pages: 374-377
    |
    Published online on: March 18, 2014
       https://doi.org/10.3892/br.2014.257
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Abstract

The aim of the present study was to investigate the effect of CpG‑oligodeoxynucelotides (CpG‑ODN) on the proliferation of the A549 human lung adenocarcinoma cell line and the expression of Runt‑related transcription factor 3 (Runx3) and investigate the association between the toll‑like receptor 9 (TLR9) signaling pathway and Runx3 expression during A549 cell proliferation. different concentrations of CpG‑ODN were used in this study to stimulate A549 cells and the expression of Runx3 at the mRNA or protein level was detected by reverse transcription-polymerase chain reaction or western blot analysis. Moreover, Runx3 siRNA was synthesized and transiently transfected into the A549 cells and the MTT assay was used to detect the effects of CpG‑ODN on transfected cell growth. Our data demonstrated that CpG‑ODN significantly inhibited the proliferation of A549 cells. The expression of Runx3 in the mRNA and protein level was increased in A549 cells stimulated by CpG‑ODN. The CpG‑ODN‑stimulated cell proliferation was significantly inhibited in Runx3 siRNA‑transfected A549 cells. In conclusion, CpG‑ODN may bind to TLR9, inhibit the proliferation of A549 cells and upregulate the expression of Runx3.
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Copy and paste a formatted citation
Spandidos Publications style
Amoah Barnie P, Zhang P, Lu P, Chen X, Su Z, Wang S and Xu H: CpG‑oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll‑like receptor 9 signaling and upregulation of Runt‑related transcription factor 3 expression. Biomed Rep 2: 374-377, 2014.
APA
Amoah Barnie, P., Zhang, P., Lu, P., Chen, X., Su, Z., Wang, S., & Xu, H. (2014). CpG‑oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll‑like receptor 9 signaling and upregulation of Runt‑related transcription factor 3 expression. Biomedical Reports, 2, 374-377. https://doi.org/10.3892/br.2014.257
MLA
Amoah Barnie, P., Zhang, P., Lu, P., Chen, X., Su, Z., Wang, S., Xu, H."CpG‑oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll‑like receptor 9 signaling and upregulation of Runt‑related transcription factor 3 expression". Biomedical Reports 2.3 (2014): 374-377.
Chicago
Amoah Barnie, P., Zhang, P., Lu, P., Chen, X., Su, Z., Wang, S., Xu, H."CpG‑oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll‑like receptor 9 signaling and upregulation of Runt‑related transcription factor 3 expression". Biomedical Reports 2, no. 3 (2014): 374-377. https://doi.org/10.3892/br.2014.257
Copy and paste a formatted citation
x
Spandidos Publications style
Amoah Barnie P, Zhang P, Lu P, Chen X, Su Z, Wang S and Xu H: CpG‑oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll‑like receptor 9 signaling and upregulation of Runt‑related transcription factor 3 expression. Biomed Rep 2: 374-377, 2014.
APA
Amoah Barnie, P., Zhang, P., Lu, P., Chen, X., Su, Z., Wang, S., & Xu, H. (2014). CpG‑oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll‑like receptor 9 signaling and upregulation of Runt‑related transcription factor 3 expression. Biomedical Reports, 2, 374-377. https://doi.org/10.3892/br.2014.257
MLA
Amoah Barnie, P., Zhang, P., Lu, P., Chen, X., Su, Z., Wang, S., Xu, H."CpG‑oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll‑like receptor 9 signaling and upregulation of Runt‑related transcription factor 3 expression". Biomedical Reports 2.3 (2014): 374-377.
Chicago
Amoah Barnie, P., Zhang, P., Lu, P., Chen, X., Su, Z., Wang, S., Xu, H."CpG‑oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll‑like receptor 9 signaling and upregulation of Runt‑related transcription factor 3 expression". Biomedical Reports 2, no. 3 (2014): 374-377. https://doi.org/10.3892/br.2014.257
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