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Article

The differentiation effect of low‑dose cytosine arabinoside is disturbed in PU.1‑knockdown K562 cells

  • Authors:
    • Hiroko Nakano
    • Akane Yanagita
    • Shinichiro Takahashi
  • View Affiliations / Copyright

    Affiliations: Division of Hematology, Kitasato University School of Allied Health Sciences, Sagamihara, Kanagawa 252‑0373, Japan
  • Pages: 564-568
    |
    Published online on: March 31, 2014
       https://doi.org/10.3892/br.2014.265
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Abstract

We recently demonstrated by using PU.1‑knockdown K562 (K562 PU.1KD) cells stably expressing PU.1 short inhibitory RNAs and PU.1‑overexpressing K562 (K562 PU.1OE) cells, that therapeutic concentrations of 5‑aza‑2'‑deoxycytidine (5‑azadc) induce erythroid differentiation of these cells and that the PU.1 expression level is closely associated with the differentiating and apoptotic effects of 5‑azadc on K562 cells. In this study, we investigated whether the effects of low‑dose cytosine arabinoside (Ara‑C), which is another erythroid differentiation inducer in K562 cells, is associated with the expression level of PU.1 in these cells. As a result, we demonstrated that the effect of Ara‑C on cell viability and differentiation, as determined by the WST‑8 assay and β‑globin mRNA expression analysis, respectively, was suppressed in K562 PU.1KD cells compared to their controls. Collectively, these findings suggest that sufficient expression of PU.1 is indispensable for the erythroid differentiation of K562 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Nakano H, Yanagita A and Takahashi S: The differentiation effect of low‑dose cytosine arabinoside is disturbed in PU.1‑knockdown K562 cells. Biomed Rep 2: 564-568, 2014.
APA
Nakano, H., Yanagita, A., & Takahashi, S. (2014). The differentiation effect of low‑dose cytosine arabinoside is disturbed in PU.1‑knockdown K562 cells. Biomedical Reports, 2, 564-568. https://doi.org/10.3892/br.2014.265
MLA
Nakano, H., Yanagita, A., Takahashi, S."The differentiation effect of low‑dose cytosine arabinoside is disturbed in PU.1‑knockdown K562 cells". Biomedical Reports 2.4 (2014): 564-568.
Chicago
Nakano, H., Yanagita, A., Takahashi, S."The differentiation effect of low‑dose cytosine arabinoside is disturbed in PU.1‑knockdown K562 cells". Biomedical Reports 2, no. 4 (2014): 564-568. https://doi.org/10.3892/br.2014.265
Copy and paste a formatted citation
x
Spandidos Publications style
Nakano H, Yanagita A and Takahashi S: The differentiation effect of low‑dose cytosine arabinoside is disturbed in PU.1‑knockdown K562 cells. Biomed Rep 2: 564-568, 2014.
APA
Nakano, H., Yanagita, A., & Takahashi, S. (2014). The differentiation effect of low‑dose cytosine arabinoside is disturbed in PU.1‑knockdown K562 cells. Biomedical Reports, 2, 564-568. https://doi.org/10.3892/br.2014.265
MLA
Nakano, H., Yanagita, A., Takahashi, S."The differentiation effect of low‑dose cytosine arabinoside is disturbed in PU.1‑knockdown K562 cells". Biomedical Reports 2.4 (2014): 564-568.
Chicago
Nakano, H., Yanagita, A., Takahashi, S."The differentiation effect of low‑dose cytosine arabinoside is disturbed in PU.1‑knockdown K562 cells". Biomedical Reports 2, no. 4 (2014): 564-568. https://doi.org/10.3892/br.2014.265
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