Abnormal promoter methylation of multiple tumor suppressor genes in human bronchial epithelial malignant cells

Liu,Chaoyang; Li,Peng; Gao,Dewei; Zhou,Pingkun; Shao,Yun; Zhang,Qi; Li,Wenbing

doi:10.3892/br.2014.268

July-August 2014 Volume 2 Issue 4

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July-August 2014 Volume 2 Issue 4

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Article

Abnormal promoter methylation of multiple tumor suppressor genes in human bronchial epithelial malignant cells

Authors:
- Chaoyang Liu
- Peng Li
- Dewei Gao
- Pingkun Zhou
- Yun Shao
- Qi Zhang
- Wenbing Li
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Affiliations: Surgical Intensive Care Unit, Clinical Division of South Building, PLA General Hospital, Beijing 100853, P.R. China, Department of Radiation Toxicology, Beijing Institute of Radiation Medicine, Beijing 100850, P.R. China, Department of Pathology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, P.R. China
Pages: 525-528
|
Published online on: April 11, 2014

https://doi.org/10.3892/br.2014.268
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Abstract

Carcinoma of the lung is the leading cause of cancer‑related mortality worldwide. In order to understand the pathogenesis of radiation‑induced lung cancer, we adopted a model of transformed human bronchial epithelial cells (BEP2D) induced by α‑particles. Methylation‑specific polymerase chain reaction was performed to detect aberrant promoter methylation of multiple tumor suppressor genes, including p14ARF, p16INK4a, O6‑methylguanine‑DNA methyltransferase, glutathione S‑transferase P1 and death‑associated protein kinase genes in the BEP2D cell line and its malignant transformant, the BERP35T1 cell line. Our results demonstrated the distinctive methylation pattern for these tumor suppressor genes in radiation‑induced malignant cells, as compared to their wild‑type counterparts. Our study revealed epigenetic signatures for the characterization of radiation‑mediated carcinogenesis and it may facilitate early diagnosis of patients at high risk for lung cancer.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Abnormal promoter methylation of multiple tumor suppressor genes in human bronchial epithelial malignant cells

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Abstract

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