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Article

Effect of cytochalasin B on 3‑O‑[14C]‑methyl‑D‑glucose or D‑[U‑14C]glucose handling by BRIN‑BD11 cells

  • Authors:
    • Sibel Cetik
    • Aigun Rzajeva
    • Willy J. Malaisse
    • Abdullah Sener
  • View Affiliations / Copyright

    Affiliations: Laboratory of Experimental Hormonology, Brussels Free University, Brussels B‑1070, Belgium
  • Pages: 513-516
    |
    Published online on: April 25, 2014
       https://doi.org/10.3892/br.2014.269
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Abstract

The present study aimed to investigate the effects of cytochalasin B (20 µM) on the uptake of 3‑O‑[14C]‑methyl‑D‑glucose or D‑[U‑14C]glucose (8.3 mM each) by BRIN‑BD11 cells. Taking into account the distribution space of tritiated water (3HOH), which was unexpectedly increased shortly after exposure of the cells to cytochalasin B and then progressively returned to its control values, and that of L‑[1‑14C]glucose, used as an extracellular marker, it was demonstrated that cytochalasin B caused a modest, but significant inhibition of the uptake of D‑glucose and its non‑metabolized analog by the BRIN‑BD11 cells. These findings resemble those observed in acinar or ductal cells of the rat submaxillary gland and displayed a relative magnitude comparable to that found for the inhibition of D‑glucose metabolism by cytochalasin B in purified pancreatic islet B cells. These findings reinforce the view that the primary site of action of cytochalasin B is located at the level of the plasma membrane.
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Copy and paste a formatted citation
Spandidos Publications style
Cetik S, Rzajeva A, Malaisse WJ and Sener A: Effect of cytochalasin B on 3‑O‑[14C]‑methyl‑D‑glucose or D‑[U‑14C]glucose handling by BRIN‑BD11 cells. Biomed Rep 2: 513-516, 2014.
APA
Cetik, S., Rzajeva, A., Malaisse, W.J., & Sener, A. (2014). Effect of cytochalasin B on 3‑O‑[14C]‑methyl‑D‑glucose or D‑[U‑14C]glucose handling by BRIN‑BD11 cells. Biomedical Reports, 2, 513-516. https://doi.org/10.3892/br.2014.269
MLA
Cetik, S., Rzajeva, A., Malaisse, W. J., Sener, A."Effect of cytochalasin B on 3‑O‑[14C]‑methyl‑D‑glucose or D‑[U‑14C]glucose handling by BRIN‑BD11 cells". Biomedical Reports 2.4 (2014): 513-516.
Chicago
Cetik, S., Rzajeva, A., Malaisse, W. J., Sener, A."Effect of cytochalasin B on 3‑O‑[14C]‑methyl‑D‑glucose or D‑[U‑14C]glucose handling by BRIN‑BD11 cells". Biomedical Reports 2, no. 4 (2014): 513-516. https://doi.org/10.3892/br.2014.269
Copy and paste a formatted citation
x
Spandidos Publications style
Cetik S, Rzajeva A, Malaisse WJ and Sener A: Effect of cytochalasin B on 3‑O‑[14C]‑methyl‑D‑glucose or D‑[U‑14C]glucose handling by BRIN‑BD11 cells. Biomed Rep 2: 513-516, 2014.
APA
Cetik, S., Rzajeva, A., Malaisse, W.J., & Sener, A. (2014). Effect of cytochalasin B on 3‑O‑[14C]‑methyl‑D‑glucose or D‑[U‑14C]glucose handling by BRIN‑BD11 cells. Biomedical Reports, 2, 513-516. https://doi.org/10.3892/br.2014.269
MLA
Cetik, S., Rzajeva, A., Malaisse, W. J., Sener, A."Effect of cytochalasin B on 3‑O‑[14C]‑methyl‑D‑glucose or D‑[U‑14C]glucose handling by BRIN‑BD11 cells". Biomedical Reports 2.4 (2014): 513-516.
Chicago
Cetik, S., Rzajeva, A., Malaisse, W. J., Sener, A."Effect of cytochalasin B on 3‑O‑[14C]‑methyl‑D‑glucose or D‑[U‑14C]glucose handling by BRIN‑BD11 cells". Biomedical Reports 2, no. 4 (2014): 513-516. https://doi.org/10.3892/br.2014.269
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