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Article

Comparison of polymerase chain reaction ribotyping, toxinotyping and nutritional aspects of toxin production of Clostridium difficile strains

  • Authors:
    • Shanshan Zhu
    • Liming Zhang
    • Chunling Zhang
    • Xianjun Chen
    • Qi Chen
    • Zhaoyun Li
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Taizhou Central Hospital, Taizhou, Zhejiang 318000, P.R. China, School of Laboratory and Life Science, Wenzhou Medical University, Wenzhou 325035, P.R. China
  • Pages: 477-480
    |
    Published online on: April 25, 2014
       https://doi.org/10.3892/br.2014.270
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Abstract

Clostridium difficile (C. difficile) is the leading cause of infectious diarrhea in hospitals worldwide. Enterotoxin A (TcdA) and cytotoxin B (TcdB), have been identified as the main virulence factors of C. difficile. In China, data on polymerase chain reaction (PCR) ribotypes and abilities of hospital‑derived C. difficile isolates to produce TcdA and TcdB are sparse. In this study, we identified 40 C. difficile isolates from the Taizhou hospital and investigated their PCR ribotypes based on the 16S‑23S rRNA gene intergenic spacer region. The ability of different ribotypes to produce TcdA and TcdB was determined by immunochromatography and cytotoxicity assays, respectively. The effects of the nutritional status on the production of these toxins were also investigated.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu S, Zhang L, Zhang C, Chen X, Chen Q and Li Z: Comparison of polymerase chain reaction ribotyping, toxinotyping and nutritional aspects of toxin production of Clostridium difficile strains. Biomed Rep 2: 477-480, 2014.
APA
Zhu, S., Zhang, L., Zhang, C., Chen, X., Chen, Q., & Li, Z. (2014). Comparison of polymerase chain reaction ribotyping, toxinotyping and nutritional aspects of toxin production of Clostridium difficile strains. Biomedical Reports, 2, 477-480. https://doi.org/10.3892/br.2014.270
MLA
Zhu, S., Zhang, L., Zhang, C., Chen, X., Chen, Q., Li, Z."Comparison of polymerase chain reaction ribotyping, toxinotyping and nutritional aspects of toxin production of Clostridium difficile strains". Biomedical Reports 2.4 (2014): 477-480.
Chicago
Zhu, S., Zhang, L., Zhang, C., Chen, X., Chen, Q., Li, Z."Comparison of polymerase chain reaction ribotyping, toxinotyping and nutritional aspects of toxin production of Clostridium difficile strains". Biomedical Reports 2, no. 4 (2014): 477-480. https://doi.org/10.3892/br.2014.270
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu S, Zhang L, Zhang C, Chen X, Chen Q and Li Z: Comparison of polymerase chain reaction ribotyping, toxinotyping and nutritional aspects of toxin production of Clostridium difficile strains. Biomed Rep 2: 477-480, 2014.
APA
Zhu, S., Zhang, L., Zhang, C., Chen, X., Chen, Q., & Li, Z. (2014). Comparison of polymerase chain reaction ribotyping, toxinotyping and nutritional aspects of toxin production of Clostridium difficile strains. Biomedical Reports, 2, 477-480. https://doi.org/10.3892/br.2014.270
MLA
Zhu, S., Zhang, L., Zhang, C., Chen, X., Chen, Q., Li, Z."Comparison of polymerase chain reaction ribotyping, toxinotyping and nutritional aspects of toxin production of Clostridium difficile strains". Biomedical Reports 2.4 (2014): 477-480.
Chicago
Zhu, S., Zhang, L., Zhang, C., Chen, X., Chen, Q., Li, Z."Comparison of polymerase chain reaction ribotyping, toxinotyping and nutritional aspects of toxin production of Clostridium difficile strains". Biomedical Reports 2, no. 4 (2014): 477-480. https://doi.org/10.3892/br.2014.270
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