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Article

Microarray comparison of the gene expression profiles in the adult vs. embryonic day 14 rat liver

  • Authors:
    • Jie Zheng
    • Shuna Yu
    • Zhengchen Jiang
    • Caixing Shi
    • Jin Li
    • Xiaodong Du
    • Hailiang Wang
    • Jiying Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Weifang Medical University, Weifang, Shandong 261053, P.R. China, Department of Anatomy, Weifang Medical University, Weifang, Shandong 261053, P.R. China, Department of Clinical Medicine, Weifang Medical University, Weifang, Shandong 261053, P.R. China
  • Pages: 664-670
    |
    Published online on: June 25, 2014
       https://doi.org/10.3892/br.2014.303
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Abstract

The aim of the present study was to identify the differentially‑expressed genes of embryonic day 14 (ED 14) rat liver in comparison to adult rat liver, which may provide specific information for the investigation of the hepatogenesis mechanism. The gene expression profiles of ED 14 and adult rat livers were investigated using microarray analysis (the Illumina RatRef‑12 Expression BeadChip). Quantitative polymerase chain reaction (qPCR) analyses were conducted to confirm the gene expression. There were 787 genes upregulated in the embryonic liver. Based on the gene ontology classification system, which was analyzed by the database for annotation, visualization and integrated discovery software, a number of the upregulated genes were categorized into the distinct and differentially‑expressed functional groups, including metabolism pathway, cell cycle, transcription, signal transduction, purine metabolism, cell structure, transportation and apoptosis. qPCR analyses confirmed the gene expression. Eleven upregulated genes were found in the ED 14 rat liver, which may provide specific information for the understanding of the molecular mechanisms that control hepatogenesis. These overexpressed genes are potential markers for identifying hepatic progenitor cells.
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Copy and paste a formatted citation
Spandidos Publications style
Zheng J, Yu S, Jiang Z, Shi C, Li J, Du X, Wang H and Jiang J: Microarray comparison of the gene expression profiles in the adult vs. embryonic day 14 rat liver. Biomed Rep 2: 664-670, 2014.
APA
Zheng, J., Yu, S., Jiang, Z., Shi, C., Li, J., Du, X. ... Jiang, J. (2014). Microarray comparison of the gene expression profiles in the adult vs. embryonic day 14 rat liver. Biomedical Reports, 2, 664-670. https://doi.org/10.3892/br.2014.303
MLA
Zheng, J., Yu, S., Jiang, Z., Shi, C., Li, J., Du, X., Wang, H., Jiang, J."Microarray comparison of the gene expression profiles in the adult vs. embryonic day 14 rat liver". Biomedical Reports 2.5 (2014): 664-670.
Chicago
Zheng, J., Yu, S., Jiang, Z., Shi, C., Li, J., Du, X., Wang, H., Jiang, J."Microarray comparison of the gene expression profiles in the adult vs. embryonic day 14 rat liver". Biomedical Reports 2, no. 5 (2014): 664-670. https://doi.org/10.3892/br.2014.303
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng J, Yu S, Jiang Z, Shi C, Li J, Du X, Wang H and Jiang J: Microarray comparison of the gene expression profiles in the adult vs. embryonic day 14 rat liver. Biomed Rep 2: 664-670, 2014.
APA
Zheng, J., Yu, S., Jiang, Z., Shi, C., Li, J., Du, X. ... Jiang, J. (2014). Microarray comparison of the gene expression profiles in the adult vs. embryonic day 14 rat liver. Biomedical Reports, 2, 664-670. https://doi.org/10.3892/br.2014.303
MLA
Zheng, J., Yu, S., Jiang, Z., Shi, C., Li, J., Du, X., Wang, H., Jiang, J."Microarray comparison of the gene expression profiles in the adult vs. embryonic day 14 rat liver". Biomedical Reports 2.5 (2014): 664-670.
Chicago
Zheng, J., Yu, S., Jiang, Z., Shi, C., Li, J., Du, X., Wang, H., Jiang, J."Microarray comparison of the gene expression profiles in the adult vs. embryonic day 14 rat liver". Biomedical Reports 2, no. 5 (2014): 664-670. https://doi.org/10.3892/br.2014.303
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