DNA profiling in peripheral blood, buccal swabs, hair follicles and semen from a patient following allogeneic hematopoietic stem cells transplantation

Li,Ya‑Ting; Xie,Ming‑Kun; Wu,Jin

doi:10.3892/br.2014.332

November-December 2014 Volume 2 Issue 6

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November-December 2014 Volume 2 Issue 6

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Article

DNA profiling in peripheral blood, buccal swabs, hair follicles and semen from a patient following allogeneic hematopoietic stem cells transplantation

Authors:
- Ya‑Ting Li
- Ming‑Kun Xie
- Jin Wu
View Affiliations / Copyright

Affiliations: Department of Forensic Genetics, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China
Pages: 804-808
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Published online on: August 5, 2014

https://doi.org/10.3892/br.2014.332
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Abstract

Allogeneic peripheral blood stem cells transplantation (allo‑PBSCT) or allogeneic bone marrow transplantation (allo‑BMT) have been widely used to treat patients exhibiting certain severe illnesses. However, previous studies have shown that the biological materials of allo‑PBSCT or allo‑BMT recipients may not constitute credible materials for personal identification. In the present study, four types of commonly used samples were collected from a male individual following gender‑matched allo‑BMT. Autosomal short tandem repeat (STR) and Y‑STR markers analysis, based on polymerase chain reaction, were used to evaluate the chimerism status. The results showed that the blood sample were all donor type, the buccal swab sample were mixed chimerism, and the sperm and hair follicle samples maintained a recipient origin of 100%. In conclusion, identical results were obtained by the two methods and it was confirmed that DNA extracted from hair follicles and sperm can be used as a reference for the pre‑transplant genotype DNA profile of the recipient in the gender‑match allo‑BMT or ‑PBSCT.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

DNA profiling in peripheral blood, buccal swabs, hair follicles and semen from a patient following allogeneic hematopoietic stem cells transplantation

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