Role of cyclooxygenase‑2 in intestinal injury in neonatal rats

Introduction

Necrotizing enterocolitis (NEC) is one of the most damaging intra-abdominal emergencies in premature infants and is the cause of significant mortality and morbidity, including neurodevelopmental impairment, particularly in extreme preterm neonates requiring surgery for the illness (1–3). Although the pathogenesis of NEC remains elusive, the principal initiating events are believed to involve gut ischemia, formula feeding and intestinal colonization with opportunistic pathogens. These perinatal insults weaken the integrity of the immature gut barrier, resulting in bacterial translocation and activation of innate immune responses (4).

Cyclooxygenase-2 (COX-2) is a rate-limiting enzyme in the synthesis of prostanoids from their precursor, arachidonic acid. Inhibitors of COX, such as glucocorticoids and nonsteroidal anti-inflammatory drugs, have been implicated as an NEC risk factor (5,6). By contrast, COX-2 is proinflammatory and therefore, irregularly high COX-2 levels may be pathogenic during intestinal inflammation (7). These data indicate that COX-2 may play protective and deleterious roles in NEC. However, the exact role of COX-2 in the pathogenesis of NEC has not been fully elucidated. In a previous study of COX-2 in NEC, no correlation was found between COX-2 expression and intestinal injury severity (8).

Therefore, the aim of the present study was to investigate the dynamic change and the potential role of COX-2 mRNA in neonatal rat with lipopolysaccharide (LPS)-induced intestinal injury, and to define whether NEC is associated with the expression of COX-2 mRNA in the mucosa of the affected intestine tissue.

Materials and methods

Animal model

Wistar rats, <24 h in age (mean weight, 6.24±0.81 g), were administered an intraperitoneal (IP) injection of 5 mg/kg Escherichia coli O55:B5 endotoxin (LPS; Sigma-Aldrich, St. Louis, MO, USA) or a similar volume of saline (9–11). All the pups were sacrificed at 1, 3, 6, 12 or 24 h after receiving LPS IP (n=8). The control pups (n=8) were sacrificed at 1 h after saline IP. The pups that succumbed prior to the collection of the specimens were excluded from the study.

Specimens collection

All the surviving animals were sacrificed via decapitation. The gastrointestinal (GI) tract was carefully removed. The small intestine was subsequently divided into two halves: jejunum and ileum. A 3-cm segment of distal ileum, which was 4 cm proximal to the ileocecal valve, from each animal was cut and fixed for histological evaluation of NEC. The remainder of the ileum was snap-frozen at −80°C for mRNA measurement.

Experimental methods and analysis marker

NEC evaluation

The segment of distal ileum was harvested, fixed in 4% paraformadehyde, embedded in paraffin, microtome-sectioned at 5 μm and counterstained with hematoxylin and eosin for histological evaluation of intestinal injury. Histological changes in the ileum were scored by a blinded investigator and were assigned a NEC score on a scale 0–4 as follows: 0, normal, intact villous epithelium with normal histology; 1, mild villous edema with epithelial sloughing confined to the tips of the villi; 2, mild midvillous necrosis; 3, moderate midvillous necrosis with crypts still readily detectable; and 4, severe necrosis of entire villi with complete absence of epithelial structures (11,12).

Reverse transcription-polymerase chain reaction (RT-PCR) for COX-2 and β-actin

Total RNA was extracted using the Biotragents™ reagent (Sino-American Biotechnology, Co., Luoyang, China) and 2 μl RNA was used to synthesize cDNA in the presence of an oligo dT 15-primer, RNase inhibitor and the avian myeloblastosis virus reverse transcriptase in a final volume of 20 μl. Sequence-specific oligonucleotide primers (Bioasia Biotechnology, Co., Ltd., Shanghai, China) were designed according to rat podocin as follows: COX-2 sense, 5′-TTC AAA TGA GAT TGT GGG AAA ATT GCT-3′; and antisense, 5′-AGA TCA TCT CTG CCT GAG TAT CTT T-3′ and β-actin sense, 5′-CAC CCT GTG CTG CTC ACC GAG GCC-3′; and antisense, 5′-CCA CAC AGA TGA CTT GCG CTC AGG-3′. The expected size of amplification was 305 base pairs (bp) for COX-2 and 314 bp for β-actin. PCR was performed in a 25-μl reaction system, which contained 3 μl cDNA, 17.1 μl ddH2O, 2.5 μl 10X PCR buffer, 2 μl 2.5 mmol/l dNTPs, 0.2 μl Taq DNA polymerase [Takara Biotechnology (Dalian), Co., Ltd., Dalian, China] and 0.1 μl of each primer. Amplification cycles of COX-2 were 95°C for 1.5 min, followed by 45 cycles at 94°C for 45 sec, 55°C for 45 sec, 72°C for 1.5 min and terminated by a final extension of 72°C for 10 min.

The PCR products were subjected to electrophoresis with 2% agarose gel and stained with ethidium bromide. The band intensity was determined by gel image analysis system (Kodak 1D; Eastman Kodak, Rochester, NY, USA). The relative mRNA concentrations were normalized for β-actin. The expression levels of COX-2 mRNA were calculated by dividing the intensity of the internal control, β-actin.

Statistical analysis

Software SPSS 19.0 for Windows (SPSS, Inc., Chicago, IL, USA) was used in all the statistical tests. Comparisons between the groups were calculated using one-way analysis of variance and all the data are expressed as mean ± standard deviation. P<0.05, was considered to indicate a statistically significant difference. The degree of correlation was described using the Spearman’s rank-correlation test.

Results

Incidence and severity of NEC

Using the histological scoring system, tissues with histological scores ≥2+ were designated positive for NEC. In the LPS-injected group, 52.5% (21/40) showed significant (P<0.01) pathological changes in ileal structure characterized as moderate (2+), severe (3+) or full necrosis (4+) compared to only a 0% (0/8) incidence of NEC in the control group. The most deteriorating change was at 12 h and the incidence of NEC was 87.5% (7/8). There was severe necrosis of the entire villi with complete absence of epithelial structures. The degree of ileal damage was also significantly (P<0.05) increased in the LPS-injected compared to the control group (Table I).

Table I Scores of the lesion on distal ileum morphology and COX-2 mRNA of neonatal rats.

Table I

Scores of the lesion on distal ileum morphology and COX-2 mRNA of neonatal rats.

Groups	Scores	COX-2 mRNA
Control (n=8)	0.12±0.17	0.14±0.03
LPS (n=8)
1 h	1.28±0.62a	0.25±0.04a
3 h	1.75±0.74a	0.93±0.01a
6 h	1.98±0.75a	1.01±0.07a
12 h	2.85±0.41a	1.24±0.01a
24 h	2.35±0.63a	1.35±0.08a

a P<0.05 vs. control group. Data are mean ± standard deviation.

{ label (or @symbol) needed for fn[@id='tfn2-br-02-06-0875'] } COX-2, cyclooxygenase-2; LPS, lipopolysaccharide.

Expression of COX-2 mRNA

The expression of COX-2 mRNA was significantly upregulated following LPS treatment (P<0.05) (Table I and Fig. 1). There was a significantly positive correlation between COX-2 mRNA expression and the grade of intestinal injury at 1, 3, 6 and 12 h (γ=0.892, 0.855, 0.770 and 0.877; P<0.05). There was a significantly negative correlation between COX-2 mRNA and severity of NEC only at 24 h (γ=−0.769, P<0.05).

Figure 1 Gene expression of cyclooxygenase-2 in the ileum of neonatal rats. A total of 2% ethidium bromide stained agarose gel molecular marker (Kodak 1D) was used as an indicator. M, DNA marker DL2000; lane 0, control group; lane 1, lipopolysaccharide (LPS) 1 h; lane 2, LPS 3 h; lane 3, LPS 6 h; lane 4, LPS 12 h; lane 5, LPS 24 h; bp, basepair.

Discussion

In the present study, significantly lower levels of COX-2 mRNA were detected in the ileal tissue in a cohort of control rats. The COX-2 mRNA expression was significantly increased following LPS injection. There was a significantly positive correlation between the expression of COX-2 mRNA and the degree of intestinal injury within 12 h after LPS injection. These results showed that an increased expression of ileal COX-2 mRNA results in a significant increase of the incidence and severity of NEC. The COX-2 mRNA expression was not significantly decreased at 24 h, and there was a significantly negative correlation with the severity of intestinal injury.

NEC is predominately a disease of premature infants. In recent years, its incidence has become more prevalent with the increasing survival of low-birth weight premature infants (13). The pathogenesis of NEC continues to be investigated, however, the unifying hypothesis includes mucosal injury of the small intestine, followed by bacterial translocation and an amplified inflammatory response to endotoxin (14).

The COX enzymes are critical in the biosynthesis of prostanoids, play significant roles in the gut and are key for intestinal epithelium maintenance (15). COX, the enzyme that catalyzes the first two steps in the biosynthesis of the prostaglandins from arachidonic acid, exists in two isoforms. COX-1 is constitutively expressed throughout the GI tract and, at least in the absence of damage or inflammation, is the major source of prostaglandin synthesis in these tissues (16). The inducible form, COX-2, is either undetectable or expressed at extremely low levels in the healthy GI tract of humans and various animals (15). However, in response to various proinflammatory stimuli, COX-2 is rapidly induced. An increase in COX-2 protein expression was noted in the perforated intestinal sections of all 36 neonates examined in the study by Chung et al (13). High intestinal COX levels have been identified in an animal model of NEC (7,13).

COX-2 was initially regarded as a target for anti-inflammatory drugs. Suppression of the activity of this enzyme reduces edema formation and hyperalgesia. Therefore, COX-2 is also a major contributor to the processes that result in resolution of inflammation. A study of paw edema in COX-2-deficient and wild-type mice identified the significance of COX-2 in the resolution of inflammation (17). COX-knockout mice are susceptible to intestinal disorders (18). The deficiency is correlated with enhanced intestinal epithelial permeability, which results in exaggerated bacterial translocation and increased mortality during peritonitis-induced sepsis (19).

Grosfeld et al (6) reported a cytoprotective role for prostaglandin E1, showing an increased NEC risk and bowel perforation in premature infants with patent ductus arteriosus (PDA) receiving indomethacin (INDO). Mortality was higher in the PDA/INDO group with NEC compared to the PDA/INDO infants without NEC. There was a significant association between toll-like receptor-4 (TLR4) signaling and COX-2 expression in the gut. TLR4 and MyD88 signaling are required for optimal proliferation and protection against apoptosis in the injured intestine (17). MyD88 deficiency has been shown to aggravate intestinal ischemia/reperfusion injury and inhibit increases in COX-2 expression and prostaglandin E2 synthesis during the development of injury (20). LPS-induced COX-2 expression stimulates the proliferation of colonocytes and repair of colonic epithelium, therefore LPS stimulation of COX-2 was protective in experimental NEC (7). The expression of COX isoforms in the duodenum is upregulated by feeding and inhibition of COX-1 or COX-2 induces ulcers in the duodenum, indicating that the two isoforms play a critical role in the protection of the intestinal mucosa (21).

The outcome following the development of selective COX-2 inhibitors, with a purpose to reduce inflammation whilst sparing the GI tract from injury, was a series of discoveries that indicated a crucial role of COX-2 in GI mucosal defense and repair. There are low levels of COX-2 expression in the healthy GI tract, however, it also significantly contributes to mucosal immunity and to the ability of the mucosa to resist injury induced by luminal irritants. The COX-2 gene quickly responds to stress and the downstream products of this enzyme are potent lipid mediators that increase the resistance to injury and regulate the dynamics of inflammation and resolution (22).

Combined, these data indicate that a quick induction of COX-2 is a general response to luminal irritation that is aimed at increasing mucosal resistance to injury and at priming for the preparation of mucosal repair in the event that injury does occur (22). Therefore, resolution of inflammation is a critical process in restoring homeostasis and COX-2 plays a crucial role in this process.

The present data showed that the expression of COX-2 mRNA was significantly upregulated following LPS injection. The aforementioned studies indicate that COX-2 plays key roles in the ability of the GI mucosa to respond to injury. COX-2 mRNA expression was significantly upregulated with the repair of intestinal injury at 24 h, suggesting the induction of COX-2 activity participates in the exacerbation of the injury and resolution of inflammation (23).

In conclusion, COX-2 plays a significant role in neonatal rats with LPS-induced intestinal injury and repair processes. Caution should be exerted concerning the potential therapeutic uses of specific inhibitors or promoters of COX-2 at the optimal phase of inflammation and further information is required to define the role of the COX/prostaglandin pathway in the pathogenesis of NEC.

References