A novel method for co‑culture with Müller cells and microglia in rat retina in vitro

Li,Li; Qu,Chen; Wang,Fang

doi:10.3892/br.2014.370

January-February 2015 Volume 3 Issue 1

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January-February 2015 Volume 3 Issue 1

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Article

A novel method for co‑culture with Müller cells and microglia in rat retina in vitro

Authors:
- Li Li
- Chen Qu
- Fang Wang
View Affiliations / Copyright

Affiliations: Department of Ophthalmology, Shanghai Tenth People's Hospital Affiliated to Tongji University, Shanghai 200072, P.R. China
Pages: 25-27
|
Published online on: October 13, 2014

https://doi.org/10.3892/br.2014.370
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Abstract

Microglia and Müller cells are glial cells of the retina and constitute a functional link between neurons and vessels. The aim of the present study was to introduce a novel method of co‑culture with Müller cells and microglia in rat retina. A camera was used to analyze all the cell changes. Immunofluorescence staining of glutamine synthetase and OX‑42 were used for the identification of Müller cells and microglial, respectively. On day 1, all the cell types from the retina were round or oval and floating in the medium. On the following days, microglial cells were adherent and proliferated. Müller cells stretched and quickly proliferated. On days 12‑15, microglial cells were floating in the medium. Following agitation, microglial cells became quickly detached from the flask walls, whereas Müller cells remained adherent. In conclusion, agitation is an effective way to separate microglial cells from Müller cells. The time of detachment and the speed of agitation are essential. Co‑culture with Müller cells and microglia in the retina is economical and useful for future methods in microglia and Müller cell research.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A novel method for co‑culture with Müller cells and microglia in rat retina in vitro

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