Effects of ionizing radiation on retinoic acid-inducible gene-I-like receptors

Yoshino,Hironori; Saitoh,Takahiro; Kozakai,Masataka; Kashiwakura,Ikuo

doi:10.3892/br.2014.377

January-February 2015 Volume 3 Issue 1

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

January-February 2015 Volume 3 Issue 1

Full Size Image

Article

Effects of ionizing radiation on retinoic acid-inducible gene-I-like receptors

Authors:
- Hironori Yoshino
- Takahiro Saitoh
- Masataka Kozakai
- Ikuo Kashiwakura
View Affiliations / Copyright

Affiliations: Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 036‑8564, Japan
Pages: 59-62
|
Published online on: November 7, 2014

https://doi.org/10.3892/br.2014.377
Expand metrics +

Abstract

Retinoic acid‑inducible gene‑I (RIG‑I)‑like receptors [RLRs; RIG‑I and melanoma differentiation‑associated gene 5 (MDA5)] sense virus‑derived RNA or a synthetic analog of double‑stranded RNA polyinosinic‑polycytidylic acid [poly(I:C)] and are responsible for host defense against viruses. However, it remains unclear whether radiation affects RLRs. Therefore, the present study investigated the effects of ionizing radiation on RIG‑I and MDA5 expression and the response to poly(I:C) using THP1 (human monocytic cell line)‑derived macrophages. Non‑ and X‑irradiated (1‑10 Gy) macrophages expressed RIG‑I and MDA5 at mRNA and protein levels and there was no significant difference in the expression levels. Non‑ and X‑irradiated macrophages expressed antiviral cytokine interferon (IFN)‑β mRNA following poly(I:C)‑low molecular weight/LyoVec™ and poly(I:C)‑high molecular weight/LyoVec™ stimulation, the agonist of RIG‑I and MDA5, respectively. In line with the results of the expression of RIG‑I and MDA5, no significant difference in the expression of IFN‑β mRNA was observed between non‑ and X‑irradiation. These results indicate that ionizing radiation hardly affects RLR expression and the response to their agonist poly(I:C) in THP1‑derived macrophages.

View Figures

View References

1	Creagh EM and O'Neill LA: TLRs, NLRs and RLRs: a trinity of pathogen sensors that co-operate in innate immunity. Trends Immunol. 27:352–357. 2006. View Article : Google Scholar : PubMed/NCBI
2	Yan H, Ohno N and Tsuji NM: The role of C-type lectin receptors in immune homeostasis. Int Immunopharmacol. 16:353–357. 2013. View Article : Google Scholar : PubMed/NCBI
3	Vabret N and Blander JM: Sensing microbial RNA in the cytosol. Front Immunol. 4:4682013. View Article : Google Scholar : PubMed/NCBI
4	Liu D, Rhebergen AM and Eisenbarth SC: Licensing adaptive immunity by NOD-like receptors. Front Immunol. 4:4862013.PubMed/NCBI
5	Matsumiya T and Stafforini DM: Function and regulation of retinoic acid-inducible gene-I. Crit Rev Immunol. 30:489–513. 2010. View Article : Google Scholar : PubMed/NCBI
6	Kato H, Takeuchi O, Mikamo-Satoh E, Hirai R, Kawai T, Matsushita K, Hiiragi A, Dermody TS, Fujita T and Akira S: Length-dependent recognition of double-stranded ribonucleic acids by retinoic acid-inducible gene-I and melanoma differentiation-associated gene 5. J Exp Med. 205:1601–1610. 2008. View Article : Google Scholar
7	Kato H, Takeuchi O, Sato S, et al: Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses. Nature. 441:101–105. 2006. View Article : Google Scholar : PubMed/NCBI
8	Yoshino H, Chiba K, Saitoh T and Kashiwakura I: Ionizing radiation affects the expression of Toll-like receptors 2 and 4 in human monocytic cells through c-Jun N-terminal kinase activation. J Radiat Res. 55:876–884. 2014. View Article : Google Scholar : PubMed/NCBI
9	Faure E, Thomas L, Xu H, Medvedev A, Equils O and Arditi M: Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation. J Immunol. 166:2018–2024. 2001. View Article : Google Scholar : PubMed/NCBI
10	Imaizumi T, Aratani S, Nakajima T, et al: Retinoic acid-inducible gene-I is induced in endothelial cells by LPS and regulates expression of COX-2. Biochem Biophys Res Commun. 292:274–279. 2002. View Article : Google Scholar : PubMed/NCBI
11	Imaizumi T, Yagihashi N, Kubota K, Yoshida H, Sakaki H, Yagihashi S, Kimura H and Satoh K: Expression of retinoic acid-inducible gene-I (RIG-I) in macrophages: possible involvement of RIG-I in atherosclerosis. J Atheroscler Thromb. 14:51–55. 2007. View Article : Google Scholar : PubMed/NCBI
12	Besch R, Poeck H, Hohenauer T, et al: Proapoptotic signaling induced by RIG-I and MDA-5 results in type I interferon-independent apoptosis in human melanoma cells. J Clin Invest. 119:2399–2411. 2009.PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effects of ionizing radiation on retinoic acid-inducible gene-I-like receptors

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Related Articles