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Article

Minocycline protects against hepatic ischemia/reperfusion injury in a rat model

  • Authors:
    • Yining Li
    • Tao Li
    • Haizhi Qi
    • Fang Yuan
  • View Affiliations / Copyright

    Affiliations: Organ Transplantation Center, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China, Department of Nephrology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 19-24
    |
    Published online on: November 11, 2014
       https://doi.org/10.3892/br.2014.381
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Abstract

Hepatic ischemia/reperfusion (I/R) injury is a common clinical problem. The present study was conducted to investigate the protective effect and mechanism of minocycline (Mino), a tetracycline with anti‑inflammatory and antioxidant properties, on I/R injury of liver in rats. In total, 54 male Sprague‑Dawley rats were randomly divided into 3 groups with 18 rats in each: Sham‑operated (control group), I/R model (I/R group) and Mino preconditioning groups (Mino group). The rats of the Mino group were administered Mino (45 mg/kg) by gastric irrigation at 36 h before surgery and were subsequently administered with 22.5 mg/kg every 12 h for the 36 h before surgery. The rats were sacrificed at 2, 6 and 24 h after reperfusion, and the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were measured. Hematoxylin/eosin staining of liver tissues was performed to detect the rat liver histological changes and the grade of liver I/R injury (Suzuki's criteria); the levels of malondialdehyde (MDA) and myeloperoxidase (MPO) were determined by spectrophotometry; hepatic tumor necrosis factor‑α (TNF‑α) and interleukin‑1β (IL‑1β) mRNA were measured by quantitative polymerase chain reaction; the Dickkopf‑1 (DKK‑1) and β‑catenin gene products of the liver were detected by western blot analysis. Mino treatment significantly ameliorated the I/R injury of the liver, as shown by decreased Suzuki scores and liver function (ALT, AST and LDH). After 2, 6 and 24 h reperfusion, compared to the I/R group the MDA and MPO levels of the Mino group decreased in the liver tissues and the levels of hepatic TNF‑α and IL‑1β mRNA were decreased too. The protein expression of hepatic DKK‑1 decreased, whereas β‑catenin increased, which indicates that the Wnt/β‑catenin pathway has been activated. In conclusion, Mino protects the liver from I/R injury mainly through reducing oxidative stress and inhibiting the release of pro‑inflammatory cytokines by activating the Wnt/β‑catenin signaling pathway in the liver.
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1 

Subhas G, Gupta A, Bakston D, et al: Protective effect of methylprednisolone on warm ischemia-reperfusion injury in a cholestatic rat liver. Am J Surg. 199:377–381. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Schauer RJ, Kalmuk S, Gerbes AL, et al: Intravenous administration of glutathione protects parenchymal and non-parenchymal liver cells against reperfusion injury following rat liver transplantation. World J Gastroenterol. 10:864–870. 2004.

3 

Su JF, Guo CJ, Wei JY, et al: Protection against hepatic ischemia-reperfusion injury in rats by oral pretreatment with quercetin. Biomed Environ Sci. 16:1–8. 2003.PubMed/NCBI

4 

Que X, Debonera F, Xie J, et al: Pattern of ischemia reperfusion injury in a mouse orthotopic liver transplant model. J Surg Res. 116:262–268. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Jaeschke H: Reperfusion injury after warm ischemia or cold storage of the liver: role of apoptotic cell death. Transplant Proc. 34:2656–2658. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Jaeschke H: Molecular mechanisms of the hepatic ischemia-reperfusion injury and preconditioning. Am J Physiol Gastrointest Liver Physiol. 284:G15–G26. 2003.PubMed/NCBI

7 

Griffin MO, Ceballos G and Villarreal FJ: Tetracycline compounds with non-antimicrobial organ protective properties: possible mechanisms of action. Pharmacol Res. 63:102–107. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Yu XD, Wu XH, Du XW, et al: Protective effects of minocycline on renal ischemia-reperfusion injury in rats. J Chongqing Med Univ. 32:1172–1174. 2007.

9 

Plane JM, Shen Y, Pleasure DE, et al: Prospects for minocycline neuroprotection. Arch Neurol. 67:1442–1448. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Abcouwer SF, Lin CM, Shanmugam S, et al: Minocycline prevents retinal inflammation and vascular permeability following ischemia-reperfusion injury. J Neuroinflammation. 10:1492013. View Article : Google Scholar : PubMed/NCBI

11 

Zhai Y, Shen XD, O'Connell R, et al: Cutting Edge: TLR4 activation mediates liver ischemia/reperfusion inflammatory response via IFN regulatory factor 3-dependent MyD88-independent pathway. J Immunol. 173:7115–7119. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Xing HC, Li LJ, Xu KJ, et al: Intestinal microflora in rats with ischemia/reperfusion liver injury. J Zhejiang Univ Sci B. 6:14–21. 2005. View Article : Google Scholar

13 

Zhou JX, Ye QF, Ming YZ, et al: Effects of PNS on expression of nuclear factor-κB and ICAM-1 in rat liver graft after ischemia reperfusion. Chin J Mod Med. 15:1330–1332. 2005.

14 

Fondevila C, Busuttil RW and Kupiec-Weglinski JW: Hepatic ischemia/reperfusion injury - a fresh look. Exp Mol Pathol. 74:86–93. 2003. View Article : Google Scholar

15 

Thobe BM, Frink M, Hildebrand F, et al: The role of MAPK in Kupffer cell toll-like receptor (TLR) 2-, TLR4-, and TLR9-mediated signaling following trauma-hemorrhage. J Cell Physiol. 210:667–675. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Tsung A, Hoffman RA, Izuishi K, et al: Hepatic ischemia/reperfusion injury involves functional TLR4 signaling in nonparenchymal cells. J Immunol. 175:7661–7668. 2005. View Article : Google Scholar

17 

Kolb M, Margetts PJ, Anthony DC, et al: Transient expression of IL-1beta induces acute lung injury and chronic repair leading to pulmonary fibrosis. J Clin Invest. 107:1529–1536. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Griffin MO, Fricovsky E, Ceballos G, et al: Tetracyclines: a pleitropic family of compounds with promising therapeutic properties. Review of the literature. Am J Physiol Cell Physiol. 299:C539–C548. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Fausto N, Campbell JS and Riehle KJ: Liver regeneration. Hepatology. 43 (2 Suppl 1):S45–S53. 2006. View Article : Google Scholar

20 

Kwon C, Arnold J, Hsiao EC, et al: Canonical Wnt signaling is a positive regulator of mammalian cardiac progenitors. Proc Natl Acad Sci USA. 104:10894–10899. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Masckauchan TN, Shawber CJ, Funahashi Y, et al: Wnt/beta-catenin signaling induces proliferation, survival and interleukin-8 in human endothelial cells. Angiogenesis. 8:43–51. 2005. View Article : Google Scholar

22 

Behari J, Yeh TH, Krauland L, et al: Liver-specific beta-catenin knockout mice exhibit defective bile acid and cholesterol homeostasis and increased susceptibility to diet-induced steatohepatitis. Am J Pathol. 176:744–753. 2010. View Article : Google Scholar

23 

Kuncewitch M, Yang WL, Molmenti E, et al: Wnt agonist attenuates liver injury and improves survival after hepatic ischemia/reperfusion. Shock. 39:3–10. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Funato Y, Michiue T, Asashima M, et al: The thioredoxin-related redox-regulating protein nucleoredoxin inhibits Wnt-beta-catenin signaling through dishevelled. Nat Cell Biol. 8:501–508. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Essers MA, de Vries-Smits LM, Barker N, et al: Functional interaction between beta-catenin and FOXO in oxidative stress signaling. Science. 308:1181–1184. 2005. View Article : Google Scholar

26 

Goode HF, Webster NR, Howdle PD, et al: Reperfusion injury, antioxidants and hemodynamics during orthotopic liver transplantation. Hepatology. 19:354–360. 1994. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Li T, Qi H and Yuan F: Minocycline protects against hepatic ischemia/reperfusion injury in a rat model. Biomed Rep 3: 19-24, 2015.
APA
Li, Y., Li, T., Qi, H., & Yuan, F. (2015). Minocycline protects against hepatic ischemia/reperfusion injury in a rat model. Biomedical Reports, 3, 19-24. https://doi.org/10.3892/br.2014.381
MLA
Li, Y., Li, T., Qi, H., Yuan, F."Minocycline protects against hepatic ischemia/reperfusion injury in a rat model". Biomedical Reports 3.1 (2015): 19-24.
Chicago
Li, Y., Li, T., Qi, H., Yuan, F."Minocycline protects against hepatic ischemia/reperfusion injury in a rat model". Biomedical Reports 3, no. 1 (2015): 19-24. https://doi.org/10.3892/br.2014.381
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Li T, Qi H and Yuan F: Minocycline protects against hepatic ischemia/reperfusion injury in a rat model. Biomed Rep 3: 19-24, 2015.
APA
Li, Y., Li, T., Qi, H., & Yuan, F. (2015). Minocycline protects against hepatic ischemia/reperfusion injury in a rat model. Biomedical Reports, 3, 19-24. https://doi.org/10.3892/br.2014.381
MLA
Li, Y., Li, T., Qi, H., Yuan, F."Minocycline protects against hepatic ischemia/reperfusion injury in a rat model". Biomedical Reports 3.1 (2015): 19-24.
Chicago
Li, Y., Li, T., Qi, H., Yuan, F."Minocycline protects against hepatic ischemia/reperfusion injury in a rat model". Biomedical Reports 3, no. 1 (2015): 19-24. https://doi.org/10.3892/br.2014.381
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