Effects of two human chorionic gonadotropin doses administered to the ovarian states during the in vitro fertilization and embryo transfer program

Ma,Mingxing; Wang,Jialin; Xu,Lijun; Zhang,Qinxi; Du,Botao; Jiang,Xiaoying; Shi,Qingli; Zhou,Lili; Li,Baoxin; Saito,Hidekazu; Kurachi,Hirohisa

doi:10.3892/br.2014.391

March-2015 Volume 3 Issue 2

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March-2015 Volume 3 Issue 2

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Article

Effects of two human chorionic gonadotropin doses administered to the ovarian states during the in vitro fertilization and embryo transfer program

Authors:
- Mingxing Ma
- Jialin Wang
- Lijun Xu
- Qinxi Zhang
- Botao Du
- Xiaoying Jiang
- Qingli Shi
- Lili Zhou
- Baoxin Li
- Hidekazu Saito
- Hirohisa Kurachi
View Affiliations / Copyright

Affiliations: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Department of Obstetrics and Gynecology, Harbin Red Cross Hospital, Harbin, Heilongjiang 150076, P.R. China, Department of Obstetrics and Gynecology, Yamagata University, School of Medicine, Yamagata 990‑9585, Japan
Pages: 215-219
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Published online on: November 20, 2014

https://doi.org/10.3892/br.2014.391
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Abstract

The aim of the present study was to examine the effects of the human chorionic gonadotropin (hCG) dose on the pulsatility indices (PI) of the intraovarian artery on the day of follicle aspiration and the oocyte quality, intrafollicular oxidative stress and luteinization. PI was also measured on the day of hCG administration. A total of 15 patients were undergoing the in vitro fertilization and embryo transfer (IVF‑ET) program. To estimate whether there was any difference between the intraovarian artery blood flow and oocyte development of the same patients treated with 5,000 or 10,000 IU hCG, the intraovarian artery blood flow was measured by transvaginal color ultrasonography pulsed wave Doppler, and the follicular fluids and the granulosa cells were collected at follicle aspiration. There were statistically significant differences between the same patients undergoing the two different hCG‑dose treatments in which the first protocol included 10,000 IU and the second protocol included 5,000 IU hCG treatment. These differences were apparent in the PI of intraovarian artery blood flow on the day of follicle aspiration (P=0.0023), in the incidence of apoptosis in cumulus (ApoC) and mural (ApoM) granulosa cells (ApoC, P=0.0077; ApoM, P=0.0128), in the total oocytes retrieved (P=0.0342) and in the follicle fluid progesterone concentration (P=0.0044). There were no significant differences between the two protocols in the PI of intraovarian artery blood flow on the day of hCG administration (P=0.4326), serum steroid on the day of follicle aspiration [serum P, P>0.9999; serum estradiol (E2), P=0.8589], follicle fluid E2 concentration (P=0.8939), mature oocyte rate (P=0.3743) and total mature oocytes retrieved (P=0.2026). In conclusion, the dose of hCG administration can significantly affect the intraovarian artery blood flow and the development of follicles and oocytes in an IVF‑ET program.

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