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Article

Comparison of the post-mortem interval on the effect of vascular responses to the activation of ionotropic and metabotropic receptors

  • Authors:
    • Elżbieta Bloch‑Bogusławska
    • Elżbieta Grześk
    • Grzegorz Grześk
  • View Affiliations / Copyright

    Affiliations: Department of Forensic Medicine, Collegium Medicum Nicolaus Copernicus University, Bydgoszcz 85‑094, Poland, Department of Pharmacology and Therapeutics, Collegium Medicum Nicolaus Copernicus University, Bydgoszcz 85‑094, Poland
  • Pages: 230-234
    |
    Published online on: December 3, 2014
       https://doi.org/10.3892/br.2014.396
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Abstract

The contractibility of blood vessels depends on their normal structure and the availability of calcium ions; it changes under the influence of numerous contracting and relaxing factors, which control the activities of various pathways of intracellular and intercellular signaling. The main aim of the study was to investigate, by means of perfusion pressure in rat tail arteries, the role of Ca2+ in vascular response to α‑1 adrenoceptor activation by phenylephrine (PHE) and Bay K8644 agonist of the L‑type calcium channel and caffeine before and after a post‑mortem interval (PMI) of 2, 4, 6 and 8 h. A phasic increase of perfusion pressure in rat tail arteries, as induced by PHE or caffeine, in Ca2+‑free solutions was used as an indicator of intracellular Ca2+ release through the inositol 1,4,5‑triphosphate and ryanodine receptor pathways, respectively. In Ca2+‑free‑ethylene glycol tetraacetic acid (EGTA)‑poly(sodium styrenesulfonate) (PSS) and in Ca2+‑EGTA‑PSS, the PHE induced elevation of perfusion pressure significantly decreased. Vascular responses to caffeine (20 mmol/l) in Ca2+‑free‑EGTA‑PSS, with an increase of PMI from 2‑8 h, did not change significantly. A similar effect was observed with vascular responses to KCl 40 mmol/l in Ca2+‑EGTA‑PSS. To confirm whether the inhibitory effect of 2, 4, 6 and 8 h PMI was mediated through the formation of NO, nitro‑L‑arginine (L‑NNA), a potent NO synthase inhibitor, was used. Exposure to L‑NNA (10‑5 M) blocked the inhibition induced by an increase of PMI. The blocked effects of L‑NNA were reversed by L‑arginine (10‑4 M). In conclusion, these patterns of change in artery responses provide insight into the post‑mortem change in the receptor‑mediated signaling components in epithelial and smooth muscle cells, and support the further study of post‑mortem vascular responses triggered by G protein‑coupled receptors (metabotropic) and channel‑linked receptors (ionotropic) as potential markers for estimating short and long‑term PMIs, respectively.
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1 

Blatter LA and Wier WG: Nitric oxide decreases [Ca2+]i in vascular smooth muscle by inhibition of the calcium current. Cell Calcium. 15:122–131. 1994. View Article : Google Scholar : PubMed/NCBI

2 

Colton CA, Fagni L and Gilbert D: The action of hydrogen peroxide on paired pulse and long term potentiation in the hippocampus. Free Radic Biol Med. 7:3–8. 1989. View Article : Google Scholar : PubMed/NCBI

3 

Kang S, Kassam N, Gauthier ML and O'Day DH: Post-mortem changes in calmodulin binding proteins in muscle and lung. Forensic Sci Int. 131:140–147. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Koh JY and Choi DW: Quantitative determination of glutamate mediated cortical neuronal injury in cell culture by lactate dehydrogenase efflux assay. J Neurosci Methods. 20:83–90. 1987. View Article : Google Scholar : PubMed/NCBI

5 

Grześk G, Wiciński M, Malinowski B, Grześk E, Manysiak S, Odrowąż-Sypniewska G, Darvish N and Birwagen M: Calcium blockers inhibit cyclosporine A-induced hyperreactivity of vascular smooth muscle cells. Mol Med Rep. 5:1469–1474. 2012.PubMed/NCBI

6 

Slupski M, Szadujkis-Szadurski L, Grześk G, Szadujkis-Szadurski R, Szadujkis-Szadurska K, Wlodarczyk Z, Masztalerz M, Piotrowiak I and Jasiński M: Guanylate cyclase activators influence reactivity of human mesenteric superior arteries retrieved and preserved in the same conditions as transplanted kidneys. Transplant Proc. 39:1350–1353. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Depre C, Fierain L and Hue L: Activation of nitric oxide synthase by ischemia in the perfused heart. Cardiovasc Res. 33:82–87. 1997. View Article : Google Scholar : PubMed/NCBI

8 

Ikonomidou C, Mosinger JL, Salles KS, Labruyere J and Olney JW: Sensitivity of the developing rat brain to hypobaric/ischemic damage parallels sensitivity to N-methyl-aspartate neurotoxicity. J Neurosci. 9:2809–2818. 1989.PubMed/NCBI

9 

Tosun M, Paul RJ and Rapoport RM: Intracellular Ca2+ elevation and contraction due to prostaglandin F2α in rat aorta. Eur J Pharmacol. 340:203–208. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Willmott NJ, Galione A and Smith PA: Nitric oxide induces intracellular Ca2+ mobilization and increases secretion of incorporated 5-hydroxytryptamine in rat pancreatic β-cells. FEBS Lett. 371:99–104. 1995. View Article : Google Scholar : PubMed/NCBI

11 

Bloch-Bogusławska E: The effect of the time of death on the reactivity of rat caudal artery regulated by Bay-K 8644 (an agonist directly affecting the Ca channel). Arch Med Sadowej Kryminol. 56:132–135. 2006.(In Polish). PubMed/NCBI

12 

Grześk G and Szadujkis-Szadurski L: Pharmacometric analysis of α 1-adrenoceptor function in rat tail artery pretreated with lipopolysaccharides. Pol J Pharmacol. 53:605–613. 2001.PubMed/NCBI

13 

Grześk G, Kozinski M, Tantry US, Wicinski M, Fabiszak T, Navarese EP, Grzesk E, Jeong YH, Gurbel PA and Kubica J: High-dose, but not low-dose, aspirin impairs anticontractile effect of ticagrelor following ADP stimulation in rat tail artery smooth muscle cells. Biomed Res Int. 2013:9282712013. View Article : Google Scholar : PubMed/NCBI

14 

Kanner J, Harel S and Granit R: Nitric oxide, an inhibitor of lipid oxidation by lipooxygenase, cyclooxygenase and hemoglobin. Lipids. 27:46–49. 1992. View Article : Google Scholar : PubMed/NCBI

15 

Coburn RF, Moreland S, Moreland RS and Baron CB: Rate-limiting energy-dependent steps controlling oxidative metabolism-contraction coupling in rabbit aorta. J Physiol. 448:473–492. 1992. View Article : Google Scholar : PubMed/NCBI

16 

Aizenman E, Hartnett KA and Reynolds IJ: Oxygen free radicals regulate NMDA receptor function via a redox modulatory site. Neuron. 5:841–846. 1990. View Article : Google Scholar : PubMed/NCBI

17 

Bredt DS, Hwang PM and Snyder SH: Localization of nitric oxide synthase indicating a neural role for nitric oxide. Nature. 347:768–770. 1990. View Article : Google Scholar : PubMed/NCBI

18 

Choi DW: Nitric oxide: foe or friend to the injured brain? Proc Natl Acad Sci USA. 90:9741–9743. 1993. View Article : Google Scholar : PubMed/NCBI

19 

Brenman JE, Chao DS, Gee SH, McGee AW, Craven SE, Santillano DR, Wu Z, Huang F, Xia H, Peters MF, et al: Interaction of nitric oxide synthase with the postsynaptic density protein PSD-95 and α 1-synthropin mediated by PDZ domains Cell. 84:757–767. 1996.PubMed/NCBI

20 

Choi DW: Glutamate neurotoxicity and diseases of the nervous system. Neuron. 1:623–634. 1988. View Article : Google Scholar : PubMed/NCBI

21 

Furchgott RF and Vanhoutte PM: Endothelium-derived relaxing and contracting factors. FASEB J. 3:2007–2018. 1989.PubMed/NCBI

22 

Szadujkis-Szadurska K, Grzesk G, Szadujkis-Szadurski L, Gajdus M and Matusiak G: Role of nitric oxide and cGMP in the modulation of vascular contraction induced by angiotensin II and Bay K8644 during ischemia/reperfusion. Exp Ther Med. 5:616–620. 2013.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Bloch‑Bogusławska E, Grześk E and Grześk G: Comparison of the post-mortem interval on the effect of vascular responses to the activation of ionotropic and metabotropic receptors. Biomed Rep 3: 230-234, 2015.
APA
Bloch‑Bogusławska, E., Grześk, E., & Grześk, G. (2015). Comparison of the post-mortem interval on the effect of vascular responses to the activation of ionotropic and metabotropic receptors. Biomedical Reports, 3, 230-234. https://doi.org/10.3892/br.2014.396
MLA
Bloch‑Bogusławska, E., Grześk, E., Grześk, G."Comparison of the post-mortem interval on the effect of vascular responses to the activation of ionotropic and metabotropic receptors". Biomedical Reports 3.2 (2015): 230-234.
Chicago
Bloch‑Bogusławska, E., Grześk, E., Grześk, G."Comparison of the post-mortem interval on the effect of vascular responses to the activation of ionotropic and metabotropic receptors". Biomedical Reports 3, no. 2 (2015): 230-234. https://doi.org/10.3892/br.2014.396
Copy and paste a formatted citation
x
Spandidos Publications style
Bloch‑Bogusławska E, Grześk E and Grześk G: Comparison of the post-mortem interval on the effect of vascular responses to the activation of ionotropic and metabotropic receptors. Biomed Rep 3: 230-234, 2015.
APA
Bloch‑Bogusławska, E., Grześk, E., & Grześk, G. (2015). Comparison of the post-mortem interval on the effect of vascular responses to the activation of ionotropic and metabotropic receptors. Biomedical Reports, 3, 230-234. https://doi.org/10.3892/br.2014.396
MLA
Bloch‑Bogusławska, E., Grześk, E., Grześk, G."Comparison of the post-mortem interval on the effect of vascular responses to the activation of ionotropic and metabotropic receptors". Biomedical Reports 3.2 (2015): 230-234.
Chicago
Bloch‑Bogusławska, E., Grześk, E., Grześk, G."Comparison of the post-mortem interval on the effect of vascular responses to the activation of ionotropic and metabotropic receptors". Biomedical Reports 3, no. 2 (2015): 230-234. https://doi.org/10.3892/br.2014.396
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