Cross‑talks between microRNAs and mRNAs in pancreatic tissues of streptozotocin‑induced type 1 diabetic mice

Tian,Caiming; Ouyang,Xiaoxi; Lv,Qing; Zhang,Yaou; Xie,Weidong

doi:10.3892/br.2015.426

May-2015 Volume 3 Issue 3

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May-2015 Volume 3 Issue 3

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Article

Cross‑talks between microRNAs and mRNAs in pancreatic tissues of streptozotocin‑induced type 1 diabetic mice

Authors:
- Caiming Tian
- Xiaoxi Ouyang
- Qing Lv
- Yaou Zhang
- Weidong Xie
View Affiliations / Copyright

Affiliations: Department of Chemistry, Tsinghua University, Beijing 100084, P.R. China, Department of Health Inspection and Quarantine, School of Public Health, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China, Shenzhen Key Laboratory of Health Science and Technology, Division of Life Science and Health, Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, P.R. China
Pages: 333-342
|
Published online on: February 12, 2015

https://doi.org/10.3892/br.2015.426
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Abstract

Network cross‑talks between microRNAs (miRNAs) and mRNAs may be useful to elucidate the pathological mechanisms of pancreatic islet cells in diabetic individuals. The aim of the present study was to investigate the cross‑talks between miRNAs and mRNAs in pancreatic tissues of streptozotocin‑induced diabetic mice through microarray and bioinformatic methods. Based on the miRNA microarray, 64 upregulated and 72 downregulated miRNAs were observed in pancreatic tissues in diabetic mice compared to the normal controls. Based on the mRNA microarrray, 507 upregulated mRNAs and 570 downregulated mRNAs were identified in pancreatic tissues in diabetic mice compared to the normal controls. Notably, there were 246 binding points between upregulated miRNA and downregulated mRNAs; simultaneously, there were 583 binding points between downregulated miRNA and upregulated mRNAs. These changed mRNA may potentially involve the following signaling pathways: Insulin secretion, pancreatic secretion, mammalian target of rapamycin signaling pathway, forkhead box O signaling pathway and phosphatidylinositol 3‑kinase‑protein kinase B signaling. The fluctuating effects of miRNAs and matched mRNAs indicated that miRNAs may have wide cross‑talks with mRNAs in pancreatic tissues of type 1 diabetic mice. The cross‑talks may play important roles in contributing to impaired islet functions and the development of diabetes. However, further functional validation should be conducted in the future.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Cross‑talks between microRNAs and mRNAs in pancreatic tissues of streptozotocin‑induced type 1 diabetic mice

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Abstract

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