Effects of high‑fat diet on 1,2‑dimethylhydrazine‑induced aberrant crypt foci and colorectal tumours in rats

Qi,Guangying; Tang,Bo; Zhou,Lihua; Jikihara,Hiroshi; Kiwata,Atsumi; Sakamoto,Yuki; Tang,Fang; Xiao,Shengjun; Wang,Zhenran; Wu,Qiuhui; Lu,Huiling; Wu,Zhen; Zeng,Sien; Shimamoto,Fumio

doi:10.3892/br.2015.433

Effects of high‑fat diet on 1,2‑dimethylhydrazine‑induced aberrant crypt foci and colorectal tumours in rats

Authors:
- Guangying Qi
- Bo Tang
- Lihua Zhou
- Hiroshi Jikihara
- Atsumi Kiwata
- Yuki Sakamoto
- Fang Tang
- Shengjun Xiao
- Zhenran Wang
- Qiuhui Wu
- Huiling Lu
- Zhen Wu
- Sien Zeng
- Fumio Shimamoto
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Affiliations: Department of Pathology and Physiopathology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China, Department of Hepatobiliary and Gastrointestinal Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China, Department of Health Sciences, Faculty of Human Culture and Science, Prefectural University of Hiroshima, Hiroshima 734‑8558, Japan
Published online on: February 18, 2015 https://doi.org/10.3892/br.2015.433
Pages: 289-294
Copyright: © Qi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Obesity is one of the leading causes of numerous types of cancer. The present study investigated the impact of a high‑fat diet on 1,2‑dimethylhydrazine (DMH)‑induced colorectal cancer (CRC) in F344 rats. A total of 16 male F344 rats aged 4 weeks were randomly divided into two groups (8 rats/group). Rats in group A were fed a basal diet with a moderate fat (MF) content, while rats in group B were fed a high‑fat diet. Upon reaching 5 weeks of age, the rats were injected subcutaneously with DMH (20 mg/kg body weight). DMH was administered once a week for 8 consecutive weeks. All the rats were sacrificed 34 weeks after the first DMH injection and dissected to obtain samples of colorectal tissues. The tissues were examined under a microscope for the presence of aberrant crypt foci (ACFs) and subjected to histopathological analysis. The results showed that at the end of the 34‑week experiment, body weights and visceral fat levels were significantly higher in the high‑fat diet group compared to the basal diet group. In addition, the incidences of colorectal ACF, adenoma and adenocarcinoma were markedly elevated in the high‑fat diet group compared to the basal diet group. These results indicate that the consumption of a high‑fat diet promotes the development and progression of CRC and the control of fat intake may prevent CRC.

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