Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Biomedical Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 2049-9434 Online ISSN: 2049-9442
Journal Cover
July-2015 Volume 3 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2015 Volume 3 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells

  • Authors:
    • Sakiko Fujii
    • Katsumi Fujimoto
    • Noriko Goto
    • Masami Kanawa
    • Takeshi Kawamoto
    • Haiou Pan
    • Petcharin Srivatanakul
    • Waralak Rakdang
    • Juthamas Pornprasitwech
    • Tania Saskianti
    • Ketut Suardita
    • Fusanori Nishimura
    • Yukio Kato
  • View Affiliations / Copyright

    Affiliations: Department of Dental Science for Health Promotion, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Hiroshima 734‑8553, Japan, Department of Dental and Medical Biochemistry, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Hiroshima 734‑8553, Japan, Department of Pediatric Dentistry, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Hiroshima 734‑8553, Japan, Natural Science Center for Basic Research and Development, Hiroshima University, Hiroshima, Hiroshima 734‑8553, Japan, Two Cells Co., Ltd., Hiroshima, Hiroshima 734‑8551, Japan, BioEden Asia Co., Ltd., Klong Luang, Pathum Thani 12120, Thailand, Department of Pediatric Dentistry, Airlangga University, Surabaya, East Java 60132, Indonesia, Department of Conservative Dentistry, Airlangga University, Surabaya, East Java 60132, Indonesia
  • Pages: 566-572
    |
    Published online on: April 29, 2015
       https://doi.org/10.3892/br.2015.456
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Dental pulp cells (DPCs) are a promising source of transplantable cells in regenerative medicine. However, DPCs have not been fully characterized at the molecular level. The aim of the present study was to distinguish DPCs from various source‑derived mesenchymal stem cells (MSCs), fibroblasts (FBs) and other cells by the expression of several DPC‑characteristic genes. DPCs were isolated from human pulp tissues by the explant method or the enzyme digestion method, and maintained with media containing 10% serum or 7.5% platelet‑rich plasma. RNA was isolated from the cells and from dental pulp tissue specimens. The mRNA levels were determined by DNA microarray and quantitative polymerase chain reaction analyses. The msh homeobox 1, msh homeobox 2, T‑box 2 and ectonucleoside triphosphate diphosphohydrolase 1 mRNA levels in DPCs were higher than that of the levels identified in the following cell types: MSCs derived from bone marrow, synovium and adipose tissue; and in cells such as FBs, osteoblasts, adipocytes and chondrocytes. The enhanced expression in DPCs was consistently observed irrespective of donor age, tooth type and culture medium. In addition, these genes were expressed at high levels in dental pulp tissue in vivo. In conclusion, this gene set may be useful in the identification and characterization of DPCs in basic studies and pulp cell‑based regeneration therapy.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Couble ML, Farges JC, Bleicher F, Perrat-Mabillon B, Boudeulle M and Magloire H: Odontoblast differentiation of human dental pulp cells in explant cultures. Calcif Tissue Int. 66:129–138. 2000. View Article : Google Scholar : PubMed/NCBI

2 

Min JH, Ko SY, Cho YB, Ryu CJ and Jang YJ: Dentinogenic potential of human adult dental pulp cells during the extended primary culture. Hum Cell. 24:43–50. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Spath L, Rotilio V, Alessandrini M, Gambara G, De Angelis L, Mancini M, Mitsiadis TA, Vivarelli E, Naro F, Filippini A, et al: Explant-derived human dental pulp stem cells enhance differentiation and proliferation potentials. J Cell Mol Med. 14:1635–1644. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Iohara K, Imabayashi K, Ishizaka R, Watanabe A, Nabekura J, Ito M, Matsushita K, Nakamura H and Nakashima M: Complete pulp regeneration after pulpectomy by transplantation of CD105+ stem cells with stromal cell-derived factor-1. Tissue Eng Part A. 17:1911–1920. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Sakai K, Yamamoto A, Matsubara K, Nakamura S, Naruse M, Yamagata M, Sakamoto K, Tauchi R, Wakao N, Imagama S, et al: Human dental pulp-derived stem cells promote locomotor recovery after complete transection of the rat spinal cord by multiple neuro-regenerative mechanisms. J Clin Invest. 122:80–90. 2012.PubMed/NCBI

6 

Gronthos S, Mankani M, Brahim J, Robey PG and Shi S: Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA. 97:13625–13630. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG and Shi S: SHED: Stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci USA. 100:5807–5812. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Jeon M, Song JS, Choi BJ, Choi HJ, Shin DM, Jung HS and Kim SO: In vitro and in vivo characteristics of stem cells from human exfoliated deciduous teeth obtained by enzymatic disaggregation and outgrowth. Arch Oral Biol. 59:1013–1023. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Lee UL, Jeon SH, Park JY and Choung PH: Effect of platelet-rich plasma on dental stem cells derived from human impacted third molars. Regen Med. 6:67–79. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K and Nakamura Y: Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations. Stem Cells. 25:1610–1617. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Fournier BP, Ferre FC, Couty L, Lataillade JJ, Gourven M, Naveau A, Coulomb B, Lafont A and Gogly B: Multipotent progenitor cells in gingival connective tissue. Tissue Eng Part A. 16:2891–2899. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Kawahara K and Shimazu A: Expression and intracellular localization of progesterone receptors in cultured human gingival fibroblasts. J Periodontal Res. 38:242–246. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Kubo H, Shimizu M, Taya Y, et al: Identification of mesenchymal stem cell (MSC)-transcription factors by microarray and knockdown analyses, and signature molecule marked MSC in bone marrow by immunohistochemistry. Genes to cells. 14:407–424. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Tsutsumi S, Shimazu A, Miyazaki K, Pan H, Koike C, Yoshida E, Takagishi K and Kato Y: Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF. Biochem Biophys Res Commun. 288:413–419. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Edgar R, Domrachev M and Lash AE: Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res. 30:207–210. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Chen Y, Bei M, Woo I, Satokata I and Maas R: Msx1 controls inductive signaling in mammalian tooth morphogenesis. Development. 122:3035–3044. 1996.PubMed/NCBI

17 

Demenis MA, Furriel RP and Leone FA: Characterization of an ectonucleoside triphosphate diphosphohydrolase 1 activity in alkaline phosphatase-depleted rat osseous plate membranes: Possible functional involvement in the calcification process. Biochim Biophys Acta. 1646:216–225. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Saadi I, Das P, Zhao M, Raj L, Ruspita I, Xia Y, Papaioannou VE and Bei M: Msx1 and Tbx2 antagonistically regulate Bmp4 expression during the bud-to-cap stage transition in tooth development. Development. 140:2697–2702. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Yoshizawa T, Takizawa F, Iizawa F, Ishibashi O and Kawashima H, Matsuda A, Endo N and Kawashima H: Homeobox protein MSX2 acts as a molecular defense mechanism for preventing ossification in ligament fibroblasts. Mol Cell Biol. 24:3460–3472. 2004. View Article : Google Scholar : PubMed/NCBI

20 

da Silva Meirelles L, Chagastelles PC and Nardi NB: Mesenchymal stem cells reside in virtually all post-natal organs and tissues. J Cell Sci. 119:2204–2213. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Shi S, Robey PG and Gronthos S: Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis. Bone. 29:532–539. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Hung CN, Mar K, Chang HC, Chiang YL, Hu HY, Lai CC, Chu RM and Ma CM: A comparison between adipose tissue and dental pulp as sources of MSCs for tooth regeneration. Biomaterials. 32:6995–7005. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Bensoussan-Trigano V, Lallemand Y, Saint Cloment C and Robert B: Msx1 and Msx2 in limb mesenchyme modulate digit number and identity. Dev Dyn. 240:1190–1202. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Chen J, Zhong Q, Wang J, Cameron RS, Borke JL, Isales CM and Bollag RJ: Microarray analysis of Tbx2-directed gene expression: A possible role in osteogenesis. Mol Cell Endocrinol. 177:43–54. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Verhoeven MC, Haase C, Christoffels VM, Weidinger G and Bakkers J: Wnt signaling regulates atrioventricular canal formation upstream of BMP and Tbx2. Birth Defects Res A Clin Mol Teratol. 91:435–440. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Farin HF, Lüdtke TH, Schmidt MK, Placzko S, Schuster-Gossler K, Petry M, Christoffels VM and Kispert A: Tbx2 terminates shh/fgf signaling in the developing mouse limb bud by direct repression of gremlin1. PLoS Genet. 9:e10034672013. View Article : Google Scholar : PubMed/NCBI

27 

Yamada Y, Fujimoto A, Ito A, Yoshimi R and Ueda M: Cluster analysis and gene expression profiles: A cDNA microarray system-based comparison between human dental pulp stem cells (hDPSCs) and human mesenchymal stem cells (hMSCs) for tissue engineering cell therapy. Biomaterials. 27:3766–3781. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Drosopoulos JH, Broekman MJ, Islam N, Maliszewski CR, Gayle RB III and Marcus AJ: Site-directed mutagenesis of human endothelial cell ecto-ADPase/soluble CD39: Requirement of glutamate 174 and serine 218 for enzyme activity and inhibition of platelet recruitment. Biochemistry. 39:6936–6943. 2000. View Article : Google Scholar : PubMed/NCBI

29 

Pulte ED, Broekman MJ, Olson KE, Drosopoulos JH, Kizer JR, Islam N and Marcus AJ: CD39/NTPDase-1 activity and expression in normal leukocytes. Thromb Res. 121:309–317. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Zhou Q, Yan J, Putheti P, et al: Isolated CD39 expression on CD4 T cells denotes both regulatory and memory populations. Am J Transplant. 9:2303–2311. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Orriss IR, Burnstock G and Arnett TR: Purinergic signalling and bone remodelling. Curr Opin Pharmacol. 10:322–330. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Fujii S, Fujimoto K, Goto N, Kanawa M, Kawamoto T, Pan H, Srivatanakul P, Rakdang W, Pornprasitwech J, Saskianti T, Saskianti T, et al: Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells. Biomed Rep 3: 566-572, 2015.
APA
Fujii, S., Fujimoto, K., Goto, N., Kanawa, M., Kawamoto, T., Pan, H. ... Kato, Y. (2015). Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells. Biomedical Reports, 3, 566-572. https://doi.org/10.3892/br.2015.456
MLA
Fujii, S., Fujimoto, K., Goto, N., Kanawa, M., Kawamoto, T., Pan, H., Srivatanakul, P., Rakdang, W., Pornprasitwech, J., Saskianti, T., Suardita, K., Nishimura, F., Kato, Y."Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells". Biomedical Reports 3.4 (2015): 566-572.
Chicago
Fujii, S., Fujimoto, K., Goto, N., Kanawa, M., Kawamoto, T., Pan, H., Srivatanakul, P., Rakdang, W., Pornprasitwech, J., Saskianti, T., Suardita, K., Nishimura, F., Kato, Y."Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells". Biomedical Reports 3, no. 4 (2015): 566-572. https://doi.org/10.3892/br.2015.456
Copy and paste a formatted citation
x
Spandidos Publications style
Fujii S, Fujimoto K, Goto N, Kanawa M, Kawamoto T, Pan H, Srivatanakul P, Rakdang W, Pornprasitwech J, Saskianti T, Saskianti T, et al: Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells. Biomed Rep 3: 566-572, 2015.
APA
Fujii, S., Fujimoto, K., Goto, N., Kanawa, M., Kawamoto, T., Pan, H. ... Kato, Y. (2015). Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells. Biomedical Reports, 3, 566-572. https://doi.org/10.3892/br.2015.456
MLA
Fujii, S., Fujimoto, K., Goto, N., Kanawa, M., Kawamoto, T., Pan, H., Srivatanakul, P., Rakdang, W., Pornprasitwech, J., Saskianti, T., Suardita, K., Nishimura, F., Kato, Y."Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells". Biomedical Reports 3.4 (2015): 566-572.
Chicago
Fujii, S., Fujimoto, K., Goto, N., Kanawa, M., Kawamoto, T., Pan, H., Srivatanakul, P., Rakdang, W., Pornprasitwech, J., Saskianti, T., Suardita, K., Nishimura, F., Kato, Y."Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells". Biomedical Reports 3, no. 4 (2015): 566-572. https://doi.org/10.3892/br.2015.456
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team