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Article

A synergistic interaction of 17‑β‑estradiol with specific cannabinoid receptor type 2 antagonist/inverse agonist on proliferation activity in primary human osteoblasts

  • Authors:
    • Marko Hojnik
    • Luka Dobovišek
    • Željko Knez
    • Polonca Ferk
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology and Experimental Toxicology, Faculty of Medicine, University of Maribor, Maribor SI‑2000, Slovenia, Faculty of Chemistry and Chemical Engineering, University of Maribor, Maribor SI‑2000, Slovenia
  • Pages: 554-558
    |
    Published online on: May 26, 2015
       https://doi.org/10.3892/br.2015.469
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Abstract

The bone remodeling process is influenced by various factors, including estrogens and transmitters of the endocannabinoid system. In osteoblasts, cannabinoid receptors 2 (CB‑2) are expressed at a much higher level compared to CB‑1 receptors. Previous studies have shown that estrogens could influence CB‑2 receptor expression. In the present study, the possible interactions of a specific CB‑2 agonist and a specific CB‑2 antagonist/inverse agonist with 17‑β‑estradiol were investigated in primary human osteoblasts (HOB). HOB cells were cultured in phenol red‑free osteoblast growth medium (37˚C, 5% CO2). In their 5th passage, HOB were exposed to different concentrations of i) 17‑β‑estradiol (1, 10 and 100 nM); ii) a specific CB‑2 agonist (R,S)‑AM1241 (1 and 7.5 µM); and iii) a specific CB‑2 antagonist/inverse agonist AM630 (10 µM) and to selected combinations of the substances. After 24 and 48 h of incubation, HOB proliferation activity was measured using a WST‑8 assay. Alkaline phosphatase activity was also evaluated using spectrophotometry. Concomitant exposure of HOB to 17‑β‑estradiol (10 nM) and to specific CB‑2 antagonist/inverse agonist (10 µM) showed similar HOB proliferation activity to HOB incubated with 17‑β‑estradiol only at a 100 nM concentration. By contrast, concomitant incubation of HOB with 17‑β‑estradiol (10 nM) and specific CB‑2 agonist (7.5 µM) resulted in decreased HOB proliferation activity as compared to HOB incubated with 17‑β‑estradiol only (10 nM). Similar findings were observed after 24 and 48 h of incubation. In all the experiments, HOB successfully passed the alkaline phosphatase differentiation test. In conclusion, for the first time a synergistic interaction between 17‑β‑estradiol and specific CB‑2 antagonist/inverse agonist was observed in HOB. Understanding the molecular pathways of this interaction would be of great importance in developing more efficient and safer drugs for treating or preventing bone diseases.
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1 

Raisz LG: Pathogenesis of osteoporosis: concepts, conflicts, and prospects. J Clin Invest. 115:3318–3325. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Reginster JY and Burlet N: Osteoporosis: a still increasing prevalence. Bone. 38 (Suppl 1):S4–S9. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Centrella M and McCarthy TL: Estrogen receptor dependent gene expression by osteoblasts - direct, indirect, circumspect, and speculative effects. Steroids. 77:174–184. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Bilezikian JP, Raisz LG and Martin TJ: Principles of bone biologyEstrogens and Progestins. Turner RT, Rickard DJ, Iwaniec UT and Spelsberg TC: 3rd. Elsevier; Amsterdam: pp. 855–885. 2008

5 

Bradford PG, Gerace KV, Roland RL and Chrzan BG: Estrogen regulation of apoptosis in osteoblasts. Physiol Behav. 99:181–185. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Howlett AC: The cannabinoid receptors. Prostaglandins Other Lipid Mediat. 68–69:619–631. 2002. View Article : Google Scholar

7 

Whyte LS, Ryberg E, Sims NA, Ridge SA, Mackie K, Greasley PJ, Ross RA and Rogers MJ: The putative cannabinoid receptor GPR55 affects osteoclast function in vitro and bone mass in vivo. Proc Natl Acad Sci USA. 106:16511–16516. 2009. View Article : Google Scholar : PubMed/NCBI

8 

van der Stelt M, Trevisani M, Vellani V, De Petrocellis L, Schiano Moriello A, Campi B, McNaughton P, et al: Anandamide acts as an intracellular messenger amplifying Ca2+ influx via TRPV1 channels. EMBO J. 24:3026–3037. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Julien B, Grenard P, Teixeira-Clerc F, Van Nhieu JT, Li L, Karsak M, Zimmer A, Mallat A and Lotersztajn S: Antifibrogenic role of the cannabinoid receptor CB2 in the liver. Gastroenterology. 128:742–755. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Steffens S, Veillard NR, Arnaud C, Pelli G, Burger F, Staub C, Karsak M, Zimmer A, Frossard JL and Mach F: Low dose oral cannabinoid therapy reduces progression of atherosclerosis in mice. Nature. 434:782–786. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Wright K, Rooney N, Feeney M, Tate J, Robertson D, Welham M and Ward S: Differential expression of cannabinoid receptors in the human colon: cannabinoids promote epithelial wound healing. Gastroenterology. 129:437–453. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Idris AI, van't Hof RJ, Greig IR, Ridge SA, Baker D, Ross RA and Ralston SH: Regulation of bone mass, bone loss and osteoclast activity by cannabinoid receptors. Nat Med. 11:774–779. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Ofek O, Karsak M, Leclerc N, Fogel M, Frenkel B, Wright K, Tam J, Attar-Namdar M, Kram V, Shohami E, et al: Peripheral cannabinoid receptor, CB2, regulates bone mass. Proc Natl Acad Sci USA. 103:696–701. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Whyte LS, Ford L, Ridge SA, Cameron GA, Rogers MJ and Ross RA: Cannabinoids and bone: endocannabinoids modulate human osteoclast function in vitro. Br J Pharmacol. 165:2584–2597. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Hanus L, Breuer A, Tchilibon S, Shiloah S, Goldenberg D, Horowitz M, Pertwee RG, Ross RA, Mechoulam R and Fride E: HU-308: a specific agonist for CB2, a peripheral cannabinoid receptor. Proc Natl Acad Sci USA. 96:14228–14233. 1999. View Article : Google Scholar : PubMed/NCBI

16 

Wartmann M, Campbell D, Subramanian A, Burstein SH and Davis RJ: The MAP kinase signal transduction pathway is activated by the endogenous cannabinoid anandamide. FEBS Lett. 359:133–136. 1995. View Article : Google Scholar : PubMed/NCBI

17 

Melck D, Rueda D, Galve-Roperh I, De Petrocellis L, Guzman M and Di Marzo V: Involvement of the cAMP/protein kinase A pathway and of mitogen-activated protein kinase in the anti-proliferative effects of anandamide in human breast cancer cells. FEBS Lett. 463:235–240. 1999. View Article : Google Scholar : PubMed/NCBI

18 

Liu J, Gao B, Mirshahi F, Sanyal AJ, Khanolkar AD, Makriyannis A and Kunos G: Functional CB1 cannabinoid receptors in human vascular endothelial cells. Biochem J. 346:835–840. 2000. View Article : Google Scholar : PubMed/NCBI

19 

Derkinderen P, Ledent C, Parmentier M and Girault JA: Cannabinoids activate p38 mitogen-activated protein kinases through CB1 receptors in hippocampus. J Neurochem. 77:957–960. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Rueda D, Galve-Roperh I, Haro A and Guzmán M: The CB1 cannabinoid receptor is coupled to the activation of c-Jun N-terminal kinase. Mol Pharmacol. 58:814–820. 2000.PubMed/NCBI

21 

Mombouli JV, Schaeffer G, Holzmann S, Kostner GM and Graier WF: Anandamide-induced mobilization of cytosolic Ca2+ in endothelial cells. Br J Pharmacol. 126:1593–1600. 1999. View Article : Google Scholar : PubMed/NCBI

22 

Rossi F, Siniscalco D, Luongo L, De Petrocellis L, Bellini G, Petrosino S, Torella M, Santoro C, Nobili B, Perrotta S, et al: The endovanilloid/endocannabinoid system in human osteoclasts: possible involvement in bone formation and resorption. Bone. 44:476–484. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Rossi F, Bellini G, Luongo L, Torella M, Mancusi S, De Petrocellis L, Petrosino S, Siniscalco D, Orlando P, Scafuro M, et al: The endovanilloid/endocannabinoid system: a new potential target for osteoporosis therapy. Bone. 48:997–1007. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Rossi F, Bellini G, Luongo L, Mancusi S, Torella M, Tortora C, Manzo I, Guida F, Nobili B, de Novellis V and Maione S: The 17-beta-oestradiol inhibits osteoclast activity by increasing the cannabinoid CB2 receptor expression. Pharmacol Res. 68:7–15. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Liu SF, Wang ZX, Yuan YE, Bing SM, Zhang BZ, Wu JZ, Wu YE and Peng XY: Hormone changes during the menstrual cycle of Chinese women. J Reprod Fertil. 76:43–52. 1986. View Article : Google Scholar : PubMed/NCBI

26 

Kousteni S, Chen JR, Bellido T, Han L, Ali AA, O'Brien CA, Plotkin L, Fu Q, Mancino AT, Wen Y, et al: Reversal of bone loss in mice by nongenotropic signaling of sex steroids. Science. 298:843–846. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Kumar P and Song ZH: Identification of raloxifene as a novel CB2 inverse agonist. Biochem Biophys Res Commun. 435:76–81. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Kumar P and Song ZH: CB2 cannabinoid receptor is a novel target for third-generation selective estrogen receptor modulators bazedoxifene and lasofoxifene. Biochem Biophys Res Commun. 443:144–149. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Idris AI, Sophocleous A, Landao-Bassonga E, van't Hof RJ and Ralston SH: Regulation of bone mass, osteoclast function, and ovariectomy-induced bone loss by the type 2 cannabinoid receptor. Endocrinology. 149:5619–5626. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Horst-Sikorska W and Wawrzyniak A: The role of hormonal therapy in osteoporosis. Endokrynol Pol. 62:61–64. 2011.PubMed/NCBI

31 

Ofek O, Attar-Namdar M, Kram V, Dvir-Ginzberg M, Mechoulam R, Zimmer A, Frenkel B, Shohami E and Bab I: CB2 cannabinoid receptor targets mitogenic Gi protein-cyclin D1 axis in osteoblasts. J Bone Miner Res. 26:308–316. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Sophocleous A, Landao-Bassonga E, Van't Hof RJ, Idris AI and Ralston SH: The type 2 cannabinoid receptor regulates bone mass and ovariectomy-induced bone loss by affecting osteoblast differentiation and bone formation. Endocrinology. 152:2141–2149. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Chlebowski RT, Anderson GL, Gass M, Lane DS, Aragaki AK, Kuller LH, Manson JE, Stefanick ML, Ockene J, Sarto GE, et al: Estrogen plus progestin and breast cancer incidence and mortality in postmenopausal women. JAMA. 304:1684–1692. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Lépine J, Audet-Walsh E, Grégoire J, Têtu B, Plante M, Ménard V, Ayotte P, Brisson J, Caron P, Villeneuve L, et al: Circulating estrogens in endometrial cancer cases and their relationship with tissular expression of key estrogen biosynthesis and metabolic pathways. J Clin Endocrinol Metab. 95:2689–2698. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Guida M, Ligresti A, De Filippis D, D'Amico A, Petrosino S, Cipriano M, Bifulco G, Simonetti S, Orlando P, Insabato L, et al: The levels of the endocannabinoid receptor CB2 and its ligand 2-arachidonoylglycerol are elevated in endometrial carcinoma. Endocrinology. 151:921–928. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Qamri Z, Preet A, Nasser MW, Bass CE, Leone G, Barsky SH and Ganju RK: Synthetic cannabinoid receptor agonists inhibit tumor growth and metastasis of breast cancer. Mol Cancer Ther. 8:3117–3129. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Lagneux C and Lamontagne D: Involvement of cannabinoids in the cardioprotection induced by lipopolysaccharide. Br J Pharmacol. 132:793–796. 2001. View Article : Google Scholar : PubMed/NCBI

38 

Odan M, Ishizuka N, Hiramatsu Y, Inagaki M, Hashizume H, Fujii Y, Mitsumori S, Morioka Y, Soga M, Deguchi M, et al: Discovery of S-777469: an orally available CB2 agonist as an antipruritic agent. Bioorg Med Chem Lett. 22:2803–2806. 2012. View Article : Google Scholar : PubMed/NCBI

39 

Rinaldi-Carmona M, Barth F, Millan J, Derocq JM, Casellas P, Congy C, Oustric D, Sarran M, Bouaboula M, Calandra B, et al: SR 144528, the first potent and selective antagonist of the CB2 cannabinoid receptor. J Pharmacol Exp Ther. 284:644–650. 1998.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Hojnik M, Dobovišek L, Knez Ž and Ferk P: A synergistic interaction of 17‑β‑estradiol with specific cannabinoid receptor type 2 antagonist/inverse agonist on proliferation activity in primary human osteoblasts. Biomed Rep 3: 554-558, 2015.
APA
Hojnik, M., Dobovišek, L., Knez, Ž., & Ferk, P. (2015). A synergistic interaction of 17‑β‑estradiol with specific cannabinoid receptor type 2 antagonist/inverse agonist on proliferation activity in primary human osteoblasts. Biomedical Reports, 3, 554-558. https://doi.org/10.3892/br.2015.469
MLA
Hojnik, M., Dobovišek, L., Knez, Ž., Ferk, P."A synergistic interaction of 17‑β‑estradiol with specific cannabinoid receptor type 2 antagonist/inverse agonist on proliferation activity in primary human osteoblasts". Biomedical Reports 3.4 (2015): 554-558.
Chicago
Hojnik, M., Dobovišek, L., Knez, Ž., Ferk, P."A synergistic interaction of 17‑β‑estradiol with specific cannabinoid receptor type 2 antagonist/inverse agonist on proliferation activity in primary human osteoblasts". Biomedical Reports 3, no. 4 (2015): 554-558. https://doi.org/10.3892/br.2015.469
Copy and paste a formatted citation
x
Spandidos Publications style
Hojnik M, Dobovišek L, Knez Ž and Ferk P: A synergistic interaction of 17‑β‑estradiol with specific cannabinoid receptor type 2 antagonist/inverse agonist on proliferation activity in primary human osteoblasts. Biomed Rep 3: 554-558, 2015.
APA
Hojnik, M., Dobovišek, L., Knez, Ž., & Ferk, P. (2015). A synergistic interaction of 17‑β‑estradiol with specific cannabinoid receptor type 2 antagonist/inverse agonist on proliferation activity in primary human osteoblasts. Biomedical Reports, 3, 554-558. https://doi.org/10.3892/br.2015.469
MLA
Hojnik, M., Dobovišek, L., Knez, Ž., Ferk, P."A synergistic interaction of 17‑β‑estradiol with specific cannabinoid receptor type 2 antagonist/inverse agonist on proliferation activity in primary human osteoblasts". Biomedical Reports 3.4 (2015): 554-558.
Chicago
Hojnik, M., Dobovišek, L., Knez, Ž., Ferk, P."A synergistic interaction of 17‑β‑estradiol with specific cannabinoid receptor type 2 antagonist/inverse agonist on proliferation activity in primary human osteoblasts". Biomedical Reports 3, no. 4 (2015): 554-558. https://doi.org/10.3892/br.2015.469
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