Elevated hsa-miR-99a levels in maternal plasma may indicate congenital heart defects

  • Authors:
    • Lars Kehler
    • Orsolya Biro
    • Levente Lazar
    • Janos Rigo
    • Balint Nagy
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  • Published online on: August 27, 2015     https://doi.org/10.3892/br.2015.510
  • Pages: 869-873
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Abstract

The current standard for prenatal screening is mostly based on biochemical marker tests and the use of ultrasonography. There is no secure stand‑alone screening marker for congenital heart defects (CHDs). MicroRNAs (miRNAs) that are associated with cardiogenesis enter the maternal peripheral bloodstream during pregnancy and allow non‑invasive prenatal testing (NIPT). The present study investigated the plasma expression profile of fetal hsa‑miR‑99a in maternal blood. Peripheral blood samples were collected from 39 pregnant patients, comprising 22 with CHD‑positive fetuses and 17 with CHD‑free controls. miRNAs were isolated from the maternal serum and reverse transcription‑quantitative polymerase chain reaction was carried out to determine the expression of hsa‑miR‑99a. While the miRNA concentrations were almost identical among the affected and control groups (5.54 vs. 6.40 ng/µl), significantly upregulated hsa‑miR‑99a levels were identified in the affected group (1.78x10‑2±3.53x10‑2 vs. 1.09x10‑3±3.55x10‑3 ng/µl, P=0.038). In conclusion, according to the present study, hsa‑miR‑99a is involved in cardiac malformation and may serve as a biomarker during fetal development, and therefore presents as a candidate for monitoring cardiomyogenesis and potential use as a NIPT-biomarker for fetal CHD.
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November-2015
Volume 3 Issue 6

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Kehler L, Biro O, Lazar L, Rigo J and Nagy B: Elevated hsa-miR-99a levels in maternal plasma may indicate congenital heart defects. Biomed Rep 3: 869-873, 2015
APA
Kehler, L., Biro, O., Lazar, L., Rigo, J., & Nagy, B. (2015). Elevated hsa-miR-99a levels in maternal plasma may indicate congenital heart defects. Biomedical Reports, 3, 869-873. https://doi.org/10.3892/br.2015.510
MLA
Kehler, L., Biro, O., Lazar, L., Rigo, J., Nagy, B."Elevated hsa-miR-99a levels in maternal plasma may indicate congenital heart defects". Biomedical Reports 3.6 (2015): 869-873.
Chicago
Kehler, L., Biro, O., Lazar, L., Rigo, J., Nagy, B."Elevated hsa-miR-99a levels in maternal plasma may indicate congenital heart defects". Biomedical Reports 3, no. 6 (2015): 869-873. https://doi.org/10.3892/br.2015.510