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Review

Application of the cell sheet technique in tissue engineering (Review)

  • Authors:
    • Guangnan Chen
    • Yiying Qi
    • Lie Niu
    • Tuoyu Di
    • Jinwei Zhong
    • Tingting Fang
    • Weiqi Yan
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China, Department of Orthopedic Surgery, Dongping County People's Hospital, Taian, Shandong 271500, P.R. China, Department of Gastroenterology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China, Key Laboratory of Carcinogenesis and Cancer Invasion, Fudan University, Ministry of Education, Liver Cancer Institute, Zhongshan Hospital, Xuhui, Shanghai 200032, P.R. China
  • Pages: 749-757
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    Published online on: September 29, 2015
       https://doi.org/10.3892/br.2015.522
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Abstract

The development and application of the tissue engineering technique has shown a significant potential in regenerative medicine. However, the limitations of conventional tissue engineering methods (cell suspensions, scaffolds and/or growth factors) restrict its application in certain fields. The novel cell sheet technique can overcome such disadvantages. Cultured cells can be harvested as intact sheets without the use of proteolytic enzymes, such as trypsin or dispase, which can result in cell damage and loss of differentiated phenotypes. The cell sheet is a complete layer, which contains extracellular matrix, ion channel, growth factor receptors, nexin and other important cell surface proteins. Mesenchymal stem cells (MSCs), which have the potential for multiple differentiation, are promising candidate seed cells for tissue engineering. The MSC sheet technique may have potential in the fields of regenerative medicine and tissue engineering in general. Additionally, induced pluripotent stem cell and embryonic stem cell‑derived cell sheets have been proposed for tissue regeneration. Currently, the application of cell sheet for tissue reconstruction includes: Direct recipient sites implantation, superposition of cell sheets to construct three‑dimensional structure for implantation, or cell sheet combined with scaffolds. The present review discusses the progress in cell sheet techniques, particularly stem cell sheet techniques, in tissue engineering.
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Copy and paste a formatted citation
Spandidos Publications style
Chen G, Qi Y, Niu L, Di T, Zhong J, Fang T and Yan W: Application of the cell sheet technique in tissue engineering (Review). Biomed Rep 3: 749-757, 2015.
APA
Chen, G., Qi, Y., Niu, L., Di, T., Zhong, J., Fang, T., & Yan, W. (2015). Application of the cell sheet technique in tissue engineering (Review). Biomedical Reports, 3, 749-757. https://doi.org/10.3892/br.2015.522
MLA
Chen, G., Qi, Y., Niu, L., Di, T., Zhong, J., Fang, T., Yan, W."Application of the cell sheet technique in tissue engineering (Review)". Biomedical Reports 3.6 (2015): 749-757.
Chicago
Chen, G., Qi, Y., Niu, L., Di, T., Zhong, J., Fang, T., Yan, W."Application of the cell sheet technique in tissue engineering (Review)". Biomedical Reports 3, no. 6 (2015): 749-757. https://doi.org/10.3892/br.2015.522
Copy and paste a formatted citation
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Spandidos Publications style
Chen G, Qi Y, Niu L, Di T, Zhong J, Fang T and Yan W: Application of the cell sheet technique in tissue engineering (Review). Biomed Rep 3: 749-757, 2015.
APA
Chen, G., Qi, Y., Niu, L., Di, T., Zhong, J., Fang, T., & Yan, W. (2015). Application of the cell sheet technique in tissue engineering (Review). Biomedical Reports, 3, 749-757. https://doi.org/10.3892/br.2015.522
MLA
Chen, G., Qi, Y., Niu, L., Di, T., Zhong, J., Fang, T., Yan, W."Application of the cell sheet technique in tissue engineering (Review)". Biomedical Reports 3.6 (2015): 749-757.
Chicago
Chen, G., Qi, Y., Niu, L., Di, T., Zhong, J., Fang, T., Yan, W."Application of the cell sheet technique in tissue engineering (Review)". Biomedical Reports 3, no. 6 (2015): 749-757. https://doi.org/10.3892/br.2015.522
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