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Article Open Access

Minocycline induces protective autophagy in vascular endothelial cells exposed to an in vitro model of ischemia/reperfusion‑induced injury

  • Authors:
    • Wenbin Dong
    • Shigeng Xiao
    • Min Cheng
    • Xiaodi Ye
    • Gaoli Zheng
  • View Affiliations / Copyright

    Affiliations: Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, P.R. China
    Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 173-177
    |
    Published online on: December 10, 2015
       https://doi.org/10.3892/br.2015.554
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Abstract

Minocycline has been reported to exhibit advantageous effects on ischemic stroke; however, the precise mechanism of minocycline remains to be established. In the present study, human umbilical vein endothelial cells (HUVECs) were subjected to in vitro simulated ischemia/reperfusion conditions to determine the potential effect of minocycline‑induced autophagy on HUVEC damage under oxygen‑glucose deprivation/reperfusion (OGD/R). The study demonstrated that minocycline enhanced autophagy in a dose‑dependent manner in HUVECs exposed to OGD/R, and only low‑dose minocycline protected HUVECs from OGD/R‑induced damage. Subsequently, 3‑methyladenine (3‑MA) was added into the culture media and the protective effect of minocycline was abolished. At the same time, it has been observed that simultaneous treatment with 3‑MA also inhibited the autophagy activity induced by minocycline. This finding could suggest that autophagy induced by minocycline serves as one of the potential protective mechanism underlying the beneficial effects of minocycline on ischemic injury.
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Copy and paste a formatted citation
Spandidos Publications style
Dong W, Xiao S, Cheng M, Ye X and Zheng G: Minocycline induces protective autophagy in vascular endothelial cells exposed to an in vitro model of ischemia/reperfusion‑induced injury. Biomed Rep 4: 173-177, 2016.
APA
Dong, W., Xiao, S., Cheng, M., Ye, X., & Zheng, G. (2016). Minocycline induces protective autophagy in vascular endothelial cells exposed to an in vitro model of ischemia/reperfusion‑induced injury. Biomedical Reports, 4, 173-177. https://doi.org/10.3892/br.2015.554
MLA
Dong, W., Xiao, S., Cheng, M., Ye, X., Zheng, G."Minocycline induces protective autophagy in vascular endothelial cells exposed to an in vitro model of ischemia/reperfusion‑induced injury". Biomedical Reports 4.2 (2016): 173-177.
Chicago
Dong, W., Xiao, S., Cheng, M., Ye, X., Zheng, G."Minocycline induces protective autophagy in vascular endothelial cells exposed to an in vitro model of ischemia/reperfusion‑induced injury". Biomedical Reports 4, no. 2 (2016): 173-177. https://doi.org/10.3892/br.2015.554
Copy and paste a formatted citation
x
Spandidos Publications style
Dong W, Xiao S, Cheng M, Ye X and Zheng G: Minocycline induces protective autophagy in vascular endothelial cells exposed to an in vitro model of ischemia/reperfusion‑induced injury. Biomed Rep 4: 173-177, 2016.
APA
Dong, W., Xiao, S., Cheng, M., Ye, X., & Zheng, G. (2016). Minocycline induces protective autophagy in vascular endothelial cells exposed to an in vitro model of ischemia/reperfusion‑induced injury. Biomedical Reports, 4, 173-177. https://doi.org/10.3892/br.2015.554
MLA
Dong, W., Xiao, S., Cheng, M., Ye, X., Zheng, G."Minocycline induces protective autophagy in vascular endothelial cells exposed to an in vitro model of ischemia/reperfusion‑induced injury". Biomedical Reports 4.2 (2016): 173-177.
Chicago
Dong, W., Xiao, S., Cheng, M., Ye, X., Zheng, G."Minocycline induces protective autophagy in vascular endothelial cells exposed to an in vitro model of ischemia/reperfusion‑induced injury". Biomedical Reports 4, no. 2 (2016): 173-177. https://doi.org/10.3892/br.2015.554
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