Morphological and functional changes of the optic nerve following traumatic optic nerve injuries in rabbits
- Authors:
- Fei Xue
- Kunming Wu
- Tianyou Wang
- You Cheng
- Manjie Jiang
- Junfeng Ji
View Affiliations
Affiliations: Department of Otolaryngology and Head Neck Surgery, Jinling Hospital, Nanjing, Jiangsu 210002, P.R. China
- Published online on: January 7, 2016 https://doi.org/10.3892/br.2016.567
-
Pages:
188-192
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Abstract
The aim of the present study was to investigate the morphological changes of the optic nerve following traumatic injuries and decompression at different times after injury, and to observe the changes of the visually evoked potentials, to identify the relevant associations between surgical opportunity and the clinical effect of traumatic optic nerve injuries. Rabbits were chosen as the animal model for the study. All the rabbits were randomly divided into five groups (A-E), representing the normal control, decompression in 48 h, in 1 week, in 2 weeks and non-decompression groups, respectively. The pattern reversal visual evoked potentials (P-VEP) and morphological changes of the optic nerve were observed. The P-VEP of each healthy rabbit revealed typical NPN contours, while NPN waves in the injured rabbits were low and flat. The latent period of the P-wave was lengthened and the amplitude was reduced. The differences of the latent period and amplitude pre- and post-trauma were statistically significant. The morphological changes were also assessed. In the normal control group, the astrocytes of the optic nerve exhibited a cylindrical form and were arranged evenly on the vertical section. The neural fibers were arranged neatly, were even following application of a dye, and the cross section exhibited a normal configuration of the blood vessel. For the 48-h decompression group, the arrangement of the astrocytes was even on the vertical section, and vacuoles, slight swelling of the nerve, exudation around the blood vessel and a small amount of astrocytic hyperplasia were observed in the damaged area. In the non-decompression group there were large areas of necrosis, clear nerve demyelination, serious exudation around the blood vessel and astrocytic hyperplasia were observed. In conclusion, the optic nerve decompression is beneficial to protect the visual function in indirect optic nerve injuries. Visual function may be improved by decompression in 48 h compared to 2 weeks. In order to prevent secondary axon injury and to protect visual functions, the decompression should be performed as soon as possible.
View References
1
|
Matteini C, Renzi G, Becelli R, Belli E
and Iannetti G: Surgical timing in orbital fracture treatment:
Experience with 108 consecutive cases. J Craniofac Surg.
15:145–150. 2004. View Article : Google Scholar : PubMed/NCBI
|
2
|
Feng Y, Luo L, Ma Z, Sun X and Hu Y: In
vivo detection of severity of optic nerve crush using
manganese-enhanced magnetic resonance imaging in rats. Chin Med J
(Engl). 127:522–527. 2014.PubMed/NCBI
|
3
|
Solomon AS, Lavie V, Hauben U, Monsonego
A, Yoles E and Schwartz M: Complete transection of rat optic nerve
while sparing the meninges and the vasculature: An experimental
model for optic nerve neuropathy and trauma. J Neurosci Methods.
70:21–25. 1996. View Article : Google Scholar : PubMed/NCBI
|
4
|
Bien A, Seidenbecher CI, Böckers TM, Sabel
BA and Kreutz MR: Apoptotic versus necrotic characteristics of
retinal ganglion cell death after partial optic nerve injury. J
Neurotrauma. 16:153–163. 1999. View Article : Google Scholar : PubMed/NCBI
|
5
|
Yoles E, Wheeler LA and Schwartz M:
Alpha2-adrenoreceptor agonists are neuroprotective in a rat model
of optic nerve degeneration. Invest Ophthalmol Vis Sci. 40:65–73.
1999.PubMed/NCBI
|
6
|
Nakamura A, Akio T, Matsuda E and Wakami
Y: Pattern visual evoked potentials in malingering. J
Neuroophthalmol. 21:42–45. 2001. View Article : Google Scholar : PubMed/NCBI
|
7
|
Yan H, Li F and Zhang L: A new and
reliable animal model for optic nerve injury. Curr Eye Res.
37:941–948. 2012. View Article : Google Scholar : PubMed/NCBI
|
8
|
Polianskiĭ VB, Evtikhin DV and Sokolov EN:
The brightness components of the visual evoked potential to color
stimuli in the rabbit. Zh Vyssh Nerv Deiat Im I P Pavlova.
49:1046–1051. 1999.(In Russian). PubMed/NCBI
|
9
|
Bain AC, Raghupathi R and Meaney DF:
Dynamic stretch correlates to both morphological abnormalities and
electrophysiological impairment in a model of traumatic axonal
injury. J Neurotrauma. 18:499–511. 2001. View Article : Google Scholar : PubMed/NCBI
|
10
|
Takano M: Axonal regeneration of retinal
ganglion cells. Nippon Ganka Gakkai Zasshi. 100:972–981. 1996.(In
Japanese). PubMed/NCBI
|
11
|
Lynch DR and Dawson TM: Secondary
mechanisms in neuronal trauma. Curr Opin Neurol. 7:510–516. 1994.
View Article : Google Scholar : PubMed/NCBI
|
12
|
Povlishock JT and Christman CW: The
pathobiology of traumatically induced axonal injury in animals and
humans: A review of current thoughts. J Neurotrauma. 12:555–564.
1995. View Article : Google Scholar : PubMed/NCBI
|
13
|
Yoles E and Muller S: NMDA-receptor
antagonist protects neurons from secondary degeneration after
partial optic crush. J Neurotrauma. 16:153–163. 1995.
|
14
|
Maxwell WL, Povlishock JT and Graham DL: A
mechanistic analysis of nondisruptive axonal injury: A review. J
Neurotrauma. 14:419–440. 1997. View Article : Google Scholar : PubMed/NCBI
|
15
|
Zimmerer R, Rana M, Schumann P and
Gellrich NC: Diagnosis and treatment of optic nerve trauma. Facial
Plast Surg. 30:518–527. 2014. View Article : Google Scholar : PubMed/NCBI
|
16
|
Goldberg RA and Steinsapir KD:
Extracranial optic canal decompression: Indications and technique.
Ophthal Plast Reconstr Surg. 12:163–170. 1996. View Article : Google Scholar : PubMed/NCBI
|
17
|
Cook MW, Levin LA, Joseph MP and Pinczower
EF: Traumatic optic neuropathy. A meta-analysis. Arch Otolaryngol
Head Neck Surg. 122:389–392. 1996. View Article : Google Scholar : PubMed/NCBI
|
18
|
Chen F, Zuo K, Feng S, Guo J, Fan Y, Shi J
and Li H: A modified surgical procedure for endoscopic optic nerve
decompression for the treatment of traumatic optic neuropathy. N Am
J Med Sci. 6:270–273. 2014.PubMed/NCBI
|