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Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats

  • Authors:
    • Tian Wang
    • Sijin Duan
    • Haiping Wang
    • Shan Sun
    • Bing Han
    • Fenghua Fu
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China, Shandong ARURA Pharmaceutical R&D Co., Ltd., Jinan, Shandong 250101, P.R. China, Department of Food Safety, School of Life Science, Yantai University, Yantai, Shandong 264005, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 161-166
    |
    Published online on: January 7, 2016
       https://doi.org/10.3892/br.2016.568
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Abstract

The present study aimed to investigate the effect and underlying mechanisms of the Ruyi Zhenbao pill on neurological function following cerebral ischemia/reperfusion in rats. Male Sprague‑Dawley rats underwent middle cerebral artery occlusion following reperfusion. The rats received intragastrically either sodium carboxymethyl cellulose (control and model groups) or Ruyi Zhenbao pill at doses of 0.2, 0.4 or 0.8 g/kg. Neurological function was assessed by cylinder, adhesive and beam‑walking tests after 14‑day Ruyi Zhenbao pill treatment. Neurogenesis and angiogenesis were detected using immunofluorescence staining. The expression levels of brain‑derived neurotrophic factor (BDNF), nerve growth factor (NGF) and vascular endothelial growth factor (VEGF) were determined by enzyme‑linked immunosorbent assays. Treatment with 0.4 and 0.8 g/kg Ruyi Zhenbao for 14 days significantly improved neurological function, and increased the number of von Willebrand Factor‑ and neuronal nuclear antigen‑positive cells in the ischemic hemisphere of rats. Ruyi Zhenbao pill treatment also significantly enhanced the expression levels of BDNF, NGF and VEGF in the ischemic hemisphere. The results demonstrated that the Ruyi Zhenbao pill improved neurological function following ischemia in rats. The mechanisms of the Ruyi Zhenbao pill are associated with increasing the expression levels of BDNF, NGF and VEGF, and subsequently promoting neurogenesis and angiogenesis in the ischemic zone.
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1 

Johnston SC, Mendis S and Mathers CD: Global variation in stroke burden and mortality: Estimates from monitoring, surveillance and modelling. Lancet Neurol. 8:345–354. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Young AR, Ali C, Duretête A and Vivien D: Neuroprotection and stroke: Time for a compromise. J Neurochem. 103:1302–1309. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Wong CH and Crack PJ: Modulation of neuro-inflammation and vascular response by oxidative stress following cerebral ischemia-reperfusion injury. Curr Med Chem. 15:1–14. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Eltzschig HK and Eckle T: Ischemia and reperfusion-from mechanism to translation. Nat Med. 17:1391–1401. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Huang J, Upadhyay UM and Tamargo RJ: Inflammation in stroke and focal cerebral ischemia. Surg Neurol. 66:232–245. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Gürsoy-Ozdemir Y, Can A and Dalkara T: Reperfusion-induced oxidative/nitrative injury to neurovascular unit after focal cerebral ischemia. Stroke. 35:1449–1453. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Arai K, Jin G, Navaratna D and Lo EH: Brain angiogenesis in developmental and pathological processes: Neurovascular injury and angiogenic recovery after stroke. FEBS J. 276:4644–4652. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Moskowitz MA, Lo EH and Iadecola C: The science of stroke: Mechanisms in search of treatments. Neuron. 67:181–198. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Raab S and Plate KH: Different networks, common growth factors: Shared growth factors and receptors of the vascular and the nervous system. Acta Neuropathol. 113:607–626. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Yao RQ, Zhang L, Wang W and Li L: Cornel iridoid glycoside promotes neurogenesis and angiogenesis and improves neurological function after focal cerebral ischemia in rats. Brain Res Bull. 79:69–76. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Gu LJ and Zhang J: Protective effect of Ruyi Zhenbao pian on experimental cerebral ischemia in rats. Chin Arch Tradi Chin Med. 31:1288–1290. 2013.(In Chinese).

12 

Liu X, Wang L and Fu J: To study the effect of Ruyi Zhenbao pill on functional recovery of post-stroke patients. Shandong Medi. 50:882010.(In Chinese).

13 

Pereira BM, Weinstein PR, Zea-Longa E and el-Fiki M: Effect of blood flow rate and donor vessel diameter on the patency of carotid venous bypass grafts in dogs. Surg Neurol. 31:195–199. 1989. View Article : Google Scholar : PubMed/NCBI

14 

Longa EZ, Weinstein PR, Carlson S and Cummins R: Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 20:84–91. 1989. View Article : Google Scholar : PubMed/NCBI

15 

Shi GX, Yang CY, Wu MM, Guan LP, Wang LP and Liu CZ: Muscle hypertonia after permanent focal cerebral ischemia in rats: A qualitative and quantitative behavioral and electrophysiological study. Int J Neurosci. 123:575–581. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Soleman S, Yip P, Leasure JL and Moon L: Sustained sensorimotor impairments after endothelin-1 induced focal cerebral ischemia (stroke) in aged rats. Exp Neurol. 222:13–24. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Allred RP, Maldonado MA, Hsu Je and Jones TA: Training the ‘less-affected’ forelimb after unilateral cortical infarcts interferes with functional recovery of the impaired forelimb in rats. Restor Neurol Neurosci. 23:297–302. 2005.PubMed/NCBI

18 

Schallert T, Upchurch M, Wilcox RE and Vaughn DM: Posture-independent sensorimotor analysis of inter-hemispheric receptor asymmetries in neostriatum. Pharmacol Biochem Behav. 18:753–759. 1983. View Article : Google Scholar : PubMed/NCBI

19 

Lin B, Levy S, Raval AP, Perez-Pinzon MA and Defazio RA: Forebrain ischemia triggers GABAergic system degeneration in substantia nigra at chronic stages in rats. Cardiovasc Psychiatry Neurol. 2010:5069522010.PubMed/NCBI

20 

Holmberg P, Liljequist S and Wägner A: Secondary brain injuries in thalamus and hippocampus after focal ischemia caused by mild, transient extradural compression of the somatosensori cortex in the rat. Curr Neurovasc Res. 6:1–11. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Moro N and Sutton RL: Beneficial effects of sodium or ethyl pyruvate after traumatic brain injury in the rat. Exp Neurol. 225:391–401. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Lyden PD, Zivin JA, Chabolla DR, Jacobs MA and Gage FH: Quantitative effects of cerebral infarction on spatial learning in rats. Exp Neurol. 116:122–132. 1992. View Article : Google Scholar : PubMed/NCBI

23 

Porritt MJ, Andersson HC, Hou L, Nilsson A, Pekna M, Pekny M and Nilsson M: Photothrombosis-induced infarction of the mouse cerebral cortex is not affected by the Nrf2-activator sulforaphane. PLoS One. 7:e410902012. View Article : Google Scholar : PubMed/NCBI

24 

Schallert T, Fleming SM, Leasure JL, Tillerson JL and Bland ST: CNS plasticity and assessment of forelimb sensorimotor outcome in unilateral rat models of stroke, cortical ablation, parkinsonism and spinal cord injury. Neuropharmacology. 39:777–787. 2000. View Article : Google Scholar : PubMed/NCBI

25 

Chang YS, Mu D, Wendland M, Sheldon RA, Vexler ZS, McQuillen PS and Ferriero DM: Erythropoietin improves functional and histological outcome in neonatal stroke. Pediatr Res. 58:106–111. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Nomura S, Kagawa Y, Kida H, Maruta Y, Imoto H, Fujii M and Suzuki M: Effects of intrathecal baclofen therapy on motor and cognitive functions in a rat model of cerebral palsy. J Neurosurg Pediatr. 9:209–215. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Park SI, Jang DK, Han YM, Sunwoo YY, Park MS, Chung YA, Maeng LS, Im R, Kim MW, Jeun SS and Jang KS: Effect of combination therapy with sodium ozagrel and panax ginseng on transient cerebral ischemia model in rats. J Biomed Biotechnol. 2010:8934012010. View Article : Google Scholar : PubMed/NCBI

28 

Gow WR, Campbell K, Meade AJ, Watt PM, Milech N, Knuckey NW and Meloni BP: Lack of neuroprotection of inhibitory peptides targeting Jun/JNK after transient focal cerebral ischemia in spontaneously hypertensive rats. J Cereb Blood Flow Metab. 31:e1–e8. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Liu YP, Seçkin H, Izci Y, Du ZW, Yan YP and Başkaya MK: Neuroprotective effects of mesenchymal stem cells derived from human embryonic stem cells in transient focal cerebral ischemia in rats. J Cereb Blood Flow Metab. 29:780–791. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Wang L, Zhang Z, Wang Y, Zhang R and Chopp M: Treatment of stroke with erythropoietin enhances neurogenesis and angiogenesis and improves neurological function in rats. Stroke. 35:1732–1737. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Huang EJ and Reichardt LF: Neurotrophins: Roles in neuronal development and function. Annu Rev Neurosci. 24:677–736. 2001. View Article : Google Scholar : PubMed/NCBI

32 

Cowansage KK, LeDoux JE and Monfils MH: Brain-derived neurotrophic factor: A dynamic gatekeeper of neural plasticity. Curr Mol Pharmacol. 3:12–29. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Kim MW, Bang MS, Han TR, Ko YJ, Yoon BW, Kim JH, Kang LM, Lee KM and Kim MH: Exercise increased BDNF and trkB in the contralateral hemisphere of the ischemic rat brain. Brain Res. 1052:16–21. 2005. View Article : Google Scholar : PubMed/NCBI

34 

Madinier A, Bertrand N, Mossiat C, Prigent-Tessier A, Beley A, Marie C and Garnier P: Microglial involvement in neuroplastic changes following focal brain ischemia in rats. PLoS One. 4:e81012009. View Article : Google Scholar : PubMed/NCBI

35 

Greenberg DA and Jin K: From angiogenesis to neuropathology. Nature. 438:954–959. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang T, Duan S, Wang H, Sun S, Han B and Fu F: Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats. Biomed Rep 4: 161-166, 2016.
APA
Wang, T., Duan, S., Wang, H., Sun, S., Han, B., & Fu, F. (2016). Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats. Biomedical Reports, 4, 161-166. https://doi.org/10.3892/br.2016.568
MLA
Wang, T., Duan, S., Wang, H., Sun, S., Han, B., Fu, F."Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats". Biomedical Reports 4.2 (2016): 161-166.
Chicago
Wang, T., Duan, S., Wang, H., Sun, S., Han, B., Fu, F."Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats". Biomedical Reports 4, no. 2 (2016): 161-166. https://doi.org/10.3892/br.2016.568
Copy and paste a formatted citation
x
Spandidos Publications style
Wang T, Duan S, Wang H, Sun S, Han B and Fu F: Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats. Biomed Rep 4: 161-166, 2016.
APA
Wang, T., Duan, S., Wang, H., Sun, S., Han, B., & Fu, F. (2016). Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats. Biomedical Reports, 4, 161-166. https://doi.org/10.3892/br.2016.568
MLA
Wang, T., Duan, S., Wang, H., Sun, S., Han, B., Fu, F."Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats". Biomedical Reports 4.2 (2016): 161-166.
Chicago
Wang, T., Duan, S., Wang, H., Sun, S., Han, B., Fu, F."Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats". Biomedical Reports 4, no. 2 (2016): 161-166. https://doi.org/10.3892/br.2016.568
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