Open Access

Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats

  • Authors:
    • Tian Wang
    • Sijin Duan
    • Haiping Wang
    • Shan Sun
    • Bing Han
    • Fenghua Fu
  • View Affiliations

  • Published online on: January 7, 2016     https://doi.org/10.3892/br.2016.568
  • Pages: 161-166
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study aimed to investigate the effect and underlying mechanisms of the Ruyi Zhenbao pill on neurological function following cerebral ischemia/reperfusion in rats. Male Sprague‑Dawley rats underwent middle cerebral artery occlusion following reperfusion. The rats received intragastrically either sodium carboxymethyl cellulose (control and model groups) or Ruyi Zhenbao pill at doses of 0.2, 0.4 or 0.8 g/kg. Neurological function was assessed by cylinder, adhesive and beam‑walking tests after 14‑day Ruyi Zhenbao pill treatment. Neurogenesis and angiogenesis were detected using immunofluorescence staining. The expression levels of brain‑derived neurotrophic factor (BDNF), nerve growth factor (NGF) and vascular endothelial growth factor (VEGF) were determined by enzyme‑linked immunosorbent assays. Treatment with 0.4 and 0.8 g/kg Ruyi Zhenbao for 14 days significantly improved neurological function, and increased the number of von Willebrand Factor‑ and neuronal nuclear antigen‑positive cells in the ischemic hemisphere of rats. Ruyi Zhenbao pill treatment also significantly enhanced the expression levels of BDNF, NGF and VEGF in the ischemic hemisphere. The results demonstrated that the Ruyi Zhenbao pill improved neurological function following ischemia in rats. The mechanisms of the Ruyi Zhenbao pill are associated with increasing the expression levels of BDNF, NGF and VEGF, and subsequently promoting neurogenesis and angiogenesis in the ischemic zone.
View Figures
View References

Related Articles

Journal Cover

February-2016
Volume 4 Issue 2

Print ISSN: 2049-9434
Online ISSN:2049-9442

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang T, Duan S, Wang H, Sun S, Han B and Fu F: Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats. Biomed Rep 4: 161-166, 2016
APA
Wang, T., Duan, S., Wang, H., Sun, S., Han, B., & Fu, F. (2016). Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats. Biomedical Reports, 4, 161-166. https://doi.org/10.3892/br.2016.568
MLA
Wang, T., Duan, S., Wang, H., Sun, S., Han, B., Fu, F."Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats". Biomedical Reports 4.2 (2016): 161-166.
Chicago
Wang, T., Duan, S., Wang, H., Sun, S., Han, B., Fu, F."Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats". Biomedical Reports 4, no. 2 (2016): 161-166. https://doi.org/10.3892/br.2016.568