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Article

Expression of calcitonin gene‑related peptide, adenosine A2a receptor and adenosine A1 receptor in experiment rat migraine models

  • Authors:
    • Wenxian Lu
    • Bin Li
    • Jinbo Chen
    • Yipeng Su
    • Xiaomeng Dong
    • Xinyang Su
    • Lixiang Gao
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Binzhou Medical University Hospital, Binzhou, Shandong 256603, P.R. China, Clinical Medical College, Binzhou Medical University, Yantai, Shandong 264003, P.R. China
  • Pages: 379-383
    |
    Published online on: February 5, 2016
       https://doi.org/10.3892/br.2016.591
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Abstract

A migraine is a disabling neurovascular disorder characterized by a unilateral throbbing headache that lasts from 4 to 72 h. The headache is often accompanied by nausea, vomiting, phonophobia and photophobia, and may be worsened by physical exercise. The trigeminovascular system (TVS) is speculated to have an important role in migraines, although the pathophysiology of this disorder remains to be elucidated. Trigeminal ganglion (TG) and spinal trigeminal nucleus caudalis (TNC) are important components of the TVS. Several clinical cases have provided evidence for the involvement of the brainstem in migraine initiation. Electrical stimulation of the trigeminal ganglion (ESTG) in rats can activate TVS during a migraine attack. Calcitonin gene‑related peptide (CGRP) is an important vasoactive compound produced following TVS activation. Numerous studies have revealed that adenosine and its receptors have an important role in pain transmission and regulation process. However, only a few studies have examined whether adenosine A2a receptor (A2aR) and adenosine A1 receptor (A1R) are involved in migraine and nociceptive pathways. In the present study, CGRP, A2aR and A1R expression levels were detected in the TG and TNC of ESTG models through reverse transcription‑quantitative polymerase chain reaction and western blot analysis. Tianshu capsule (TSC), a type of Chinese medicine, was also used in the ESTG rat models to examine its influence on the three proteins. Results demonstrated that CGRP, A2aR and A1R mediated pain transmission and the regulation process during migraine and the expression of the three proteins was regulated by TSC.
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Copy and paste a formatted citation
Spandidos Publications style
Lu W, Li B, Chen J, Su Y, Dong X, Su X and Gao L: Expression of calcitonin gene‑related peptide, adenosine A2a receptor and adenosine A1 receptor in experiment rat migraine models. Biomed Rep 4: 379-383, 2016.
APA
Lu, W., Li, B., Chen, J., Su, Y., Dong, X., Su, X., & Gao, L. (2016). Expression of calcitonin gene‑related peptide, adenosine A2a receptor and adenosine A1 receptor in experiment rat migraine models. Biomedical Reports, 4, 379-383. https://doi.org/10.3892/br.2016.591
MLA
Lu, W., Li, B., Chen, J., Su, Y., Dong, X., Su, X., Gao, L."Expression of calcitonin gene‑related peptide, adenosine A2a receptor and adenosine A1 receptor in experiment rat migraine models". Biomedical Reports 4.3 (2016): 379-383.
Chicago
Lu, W., Li, B., Chen, J., Su, Y., Dong, X., Su, X., Gao, L."Expression of calcitonin gene‑related peptide, adenosine A2a receptor and adenosine A1 receptor in experiment rat migraine models". Biomedical Reports 4, no. 3 (2016): 379-383. https://doi.org/10.3892/br.2016.591
Copy and paste a formatted citation
x
Spandidos Publications style
Lu W, Li B, Chen J, Su Y, Dong X, Su X and Gao L: Expression of calcitonin gene‑related peptide, adenosine A2a receptor and adenosine A1 receptor in experiment rat migraine models. Biomed Rep 4: 379-383, 2016.
APA
Lu, W., Li, B., Chen, J., Su, Y., Dong, X., Su, X., & Gao, L. (2016). Expression of calcitonin gene‑related peptide, adenosine A2a receptor and adenosine A1 receptor in experiment rat migraine models. Biomedical Reports, 4, 379-383. https://doi.org/10.3892/br.2016.591
MLA
Lu, W., Li, B., Chen, J., Su, Y., Dong, X., Su, X., Gao, L."Expression of calcitonin gene‑related peptide, adenosine A2a receptor and adenosine A1 receptor in experiment rat migraine models". Biomedical Reports 4.3 (2016): 379-383.
Chicago
Lu, W., Li, B., Chen, J., Su, Y., Dong, X., Su, X., Gao, L."Expression of calcitonin gene‑related peptide, adenosine A2a receptor and adenosine A1 receptor in experiment rat migraine models". Biomedical Reports 4, no. 3 (2016): 379-383. https://doi.org/10.3892/br.2016.591
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