Presence of free triiodothyronine and free thyroxine in thyroid follicles may be correlated with the quick secretion of thyroid hormones under certain physiological conditions

Shi,Haihong; Lin,Wanrong; Liang,Bo; Cai,Huiyao; Cai,Qingyan; Shi,Yaxiong; Huang,Huibin

doi:10.3892/br.2016.596

April-2016 Volume 4 Issue 4

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April-2016 Volume 4 Issue 4

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Article

Presence of free triiodothyronine and free thyroxine in thyroid follicles may be correlated with the quick secretion of thyroid hormones under certain physiological conditions

Authors:
- Haihong Shi
- Wanrong Lin
- Bo Liang
- Huiyao Cai
- Qingyan Cai
- Yaxiong Shi
- Huibin Huang
View Affiliations / Copyright

Affiliations: Department of Oncological Surgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China, The Second Clinical Medical College of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China, Department of Endocrinology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China
Pages: 467-470
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Published online on: February 10, 2016

https://doi.org/10.3892/br.2016.596
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Abstract

Thyroid cells are polarized and the follicle structure, consisting of follicle epithelial cells, is a prerequisite for thyroid hormone synthesis. However, a reliable in vitro model simulating thyroid function is not currently available. To the best of our knowledge, the present study reports for the first time a simulated follicle by inoculation of human thyroid cells on the filter in a Transwell plate to maintain the polarity of thyroid cells. The iodine uptake was analyzed by arsenic and cerium catalysis spectrophotometry, as well as the secretion of free triiodothyronine (FT3) and free thyroxine (FT4) by direct chemiluminescence. The data showed that thyroid cells growing in the Transwell chamber synthesized and secreted FT3 and FT4, while the monolayer cells directly seeded in the 6‑well‑plate did not produce these two thyroid hormones. Regarding the iodine uptake, cells in the Transwell chamber demonstrated a markedly higher capability than the monolayer cells. The data proved that the polarity of thyroid cells could be restored using the Transwell plate, which was critical for iodine uptake and thyroid hormone synthesis. The presence of FT3 and FT4 in follicles may be correlated with the quick secretion of thyroid hormones under certain physiological conditions.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Presence of free triiodothyronine and free thyroxine in thyroid follicles may be correlated with the quick secretion of thyroid hormones under certain physiological conditions

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