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Article

Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons

  • Authors:
    • Meiqun Sun
    • Hongli Liu
    • Shengping Min
    • Hongtao Wang
    • Xiaojing Wang
  • View Affiliations / Copyright

    Affiliations: Anhui Clinical and Preclinical Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233030, P.R. China, Department of Gynecological Oncology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233030, P.R. China, Department of Immunology, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China
  • Pages: 417-420
    |
    Published online on: February 17, 2016
       https://doi.org/10.3892/br.2016.602
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Abstract

Ciliary neurotrophic factor (CNTF) is involved in the activation of astrocytes. A previous study showed that CNTF-treated astrocyte-conditioned medium (CNTF-ACM) contributed to the increase of the calcium current and the elevation of corresponding ion channels in cortical neurons. On this basis, it is reasonable to assume that CNTF-ACM may increase the intracellular free calcium concentration ([Ca2+]i) in neurons. In the present study, the effects of CNTF-ACM on [Ca2+]i in rat cortical neurons were determined, and on this basis, the aim was to investigate the potential active ingredients in ACM that are responsible for this biological process. As expected, the data indicated that CNTF-ACM resulted in a clear elevation of [Ca2+]i in neurons. Additionally, the fibroblast growth factor-2 (FGF-2) contained in the CNTF-ACM was found to participate in the upregulation of [Ca2+]i. Taken together, CNTF induces the production of active factors (at least including FGF-2) released from astrocytes, which finally potentiate the increase of [Ca2+]i in cortical neurons.
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Copy and paste a formatted citation
Spandidos Publications style
Sun M, Liu H, Min S, Wang H and Wang X: Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons. Biomed Rep 4: 417-420, 2016.
APA
Sun, M., Liu, H., Min, S., Wang, H., & Wang, X. (2016). Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons. Biomedical Reports, 4, 417-420. https://doi.org/10.3892/br.2016.602
MLA
Sun, M., Liu, H., Min, S., Wang, H., Wang, X."Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons". Biomedical Reports 4.4 (2016): 417-420.
Chicago
Sun, M., Liu, H., Min, S., Wang, H., Wang, X."Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons". Biomedical Reports 4, no. 4 (2016): 417-420. https://doi.org/10.3892/br.2016.602
Copy and paste a formatted citation
x
Spandidos Publications style
Sun M, Liu H, Min S, Wang H and Wang X: Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons. Biomed Rep 4: 417-420, 2016.
APA
Sun, M., Liu, H., Min, S., Wang, H., & Wang, X. (2016). Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons. Biomedical Reports, 4, 417-420. https://doi.org/10.3892/br.2016.602
MLA
Sun, M., Liu, H., Min, S., Wang, H., Wang, X."Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons". Biomedical Reports 4.4 (2016): 417-420.
Chicago
Sun, M., Liu, H., Min, S., Wang, H., Wang, X."Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons". Biomedical Reports 4, no. 4 (2016): 417-420. https://doi.org/10.3892/br.2016.602
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