Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons

Sun,Meiqun; Liu,Hongli; Min,Shengping; Wang,Hongtao; Wang,Xiaojing

doi:10.3892/br.2016.602

Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons

Authors:
- Meiqun Sun
- Hongli Liu
- Shengping Min
- Hongtao Wang
- Xiaojing Wang
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Affiliations: Anhui Clinical and Preclinical Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233030, P.R. China, Department of Gynecological Oncology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233030, P.R. China, Department of Immunology, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China
Published online on: February 17, 2016 https://doi.org/10.3892/br.2016.602
Pages: 417-420

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Abstract

Ciliary neurotrophic factor (CNTF) is involved in the activation of astrocytes. A previous study showed that CNTF-treated astrocyte-conditioned medium (CNTF-ACM) contributed to the increase of the calcium current and the elevation of corresponding ion channels in cortical neurons. On this basis, it is reasonable to assume that CNTF-ACM may increase the intracellular free calcium concentration ([Ca2+]i) in neurons. In the present study, the effects of CNTF-ACM on [Ca2+]i in rat cortical neurons were determined, and on this basis, the aim was to investigate the potential active ingredients in ACM that are responsible for this biological process. As expected, the data indicated that CNTF-ACM resulted in a clear elevation of [Ca2+]i in neurons. Additionally, the fibroblast growth factor-2 (FGF-2) contained in the CNTF-ACM was found to participate in the upregulation of [Ca2+]i. Taken together, CNTF induces the production of active factors (at least including FGF-2) released from astrocytes, which finally potentiate the increase of [Ca2+]i in cortical neurons.

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1	Adler R, Landa KB, Manthorpe M and Varon S: Cholinergic neuronotrophic factors: Intraocular distribution of trophic activity for ciliary neurons. Science. 204:1434–1436. 1979. View Article : Google Scholar : PubMed/NCBI
2	Askvig JM and Watt JA: The MAPK and PI3K pathways mediate CNTF-induced neuronal survival and process outgrowth in hypothalamic organotypic cultures. J Cell Commun Signal. 9:217–231. 2015. View Article : Google Scholar : PubMed/NCBI
3	Chowdhury SR, Saleh A, Akude E, Smith DR, Morrow D, Tessler L, Calcutt NA and Fernyhough P: Ciliary neurotrophic factor reverses aberrant mitochondrial bioenergetics through the JAK/STAT pathway in cultured sensory neurons derived from streptozotocin-induced diabetic rodents. Cell Mol Neurobiol. 34:643–649. 2014. View Article : Google Scholar : PubMed/NCBI
4	Vigneswara V, Akpan N, Berry M, Logan A, Troy CM and Ahmed Z: Combined suppression of CASP2 and CASP6 protects retinal ganglion cells from apoptosis and promotes axon regeneration through CNTF-mediated JAK/STAT signalling. Brain. 137:1656–1675. 2014. View Article : Google Scholar : PubMed/NCBI
5	Seidel JL, Faideau M, Aiba I, Pannasch U, Escartin C, Rouach N, Bonvento G and Shuttleworth CW: Ciliary neurotrophic factor (CNTF) activation of astrocytes decreases spreading depolarization susceptibility and increases potassium clearance. Glia. 63:91–103. 2015. View Article : Google Scholar : PubMed/NCBI
6	Wang X, Zheng H, Liu C, Zhu C, Wang W and Li Z: Ciliary neurotrophic factor-treated astrocyte conditioned medium regulates the L-type calcium channel activity in rat cortical neurons. Neurochem Res. 33:826–832. 2008. View Article : Google Scholar : PubMed/NCBI
7	Kajitani N, Hisaoka-Nakashima K, Morioka N, Okada-Tsuchioka M, Kaneko M, Kasai M, Shibasaki C, Nakata Y and Takebayashi M: Antidepressant acts on astrocytes leading to an increase in the expression of neurotrophic/growth factors: Differential regulation of FGF-2 by noradrenaline. PLoS One. 7:e511972012. View Article : Google Scholar : PubMed/NCBI
8	Liberto CM, Albrecht PJ, Herx LM, Yong VW and Levison SW: Pro-regenerative properties of cytokine-activated astrocytes. J Neurochem. 89:1092–1100. 2004. View Article : Google Scholar : PubMed/NCBI
9	Katsuki H, Shitaka Y, Saito H and Matsuki N: A potential role of Ras-mediated signal transduction for the enhancement of depolarization-induced Ca²⁺ responses in hippocampal neurons by basic fibroblast growth factor. Brain Res Dev Brain Res. 111:169–176. 1998. View Article : Google Scholar : PubMed/NCBI
10	Yagami T, Takase K, Yamamoto Y, Ueda K, Takasu N, Okamura N, Sakaeda T and Fujimoto M: Fibroblast growth factor 2 induces apoptosis in the early primary culture of rat cortical neurons. Exp Cell Res. 316:2278–2290. 2010. View Article : Google Scholar : PubMed/NCBI
11	Hall KE, Sheng HC, Srinivasan S, Spitsbergen JM, Tuttle JB, Steers WD and Wiley JW: Treatment of aged rat sensory neurons in short-term, serum-free culture with nerve growth factor reverses the effect of aging on neurite outgrowth, calcium currents, and neuronal survival. Brain Res. 888:128–137. 2001. View Article : Google Scholar : PubMed/NCBI
12	Vivas O, Kruse M and Hille B: Nerve growth factor sensitizes adult sympathetic neurons to the proinflammatory peptide bradykinin. J Neurosci. 34:11959–11971. 2014. View Article : Google Scholar : PubMed/NCBI
13	McCarthy KD and de Vellis J: Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J Cell Biol. 85:890–902. 1980. View Article : Google Scholar : PubMed/NCBI
14	Zsembery A, Boyce AT, Liang L, Peti-Peterdi J, Bell PD and Schwiebert EM: Sustained calcium entry through P2X nucleotide receptor channels in human airway epithelial cells. J Biol Chem. 278:13398–13408. 2003. View Article : Google Scholar : PubMed/NCBI
15	Ojeda SR, Ma YJ, Lee BJ and Prevot V: Glia-to-neuron signaling and the neuroendocrine control of female puberty. Recent Prog Horm Res. 55:197–224. 2000.PubMed/NCBI
16	Lu X, Al-Aref R, Zhao D, Shen J, Yan Y and Gao Y: Astrocyte-conditioned medium attenuates glutamate-induced apoptotic cell death in primary cultured spinal cord neurons of rats. Neurol Res. 37:803–808. 2015. View Article : Google Scholar : PubMed/NCBI
17	Taylor AR, Gifondorwa DJ, Newbern JM, Robinson MB, Strupe JL, Prevette D, Oppenheim RW and Milligan CE: Astrocyte and muscle-derived secreted factors differentially regulate motoneuron survival. J Neurosci. 27:634–644. 2007. View Article : Google Scholar : PubMed/NCBI
18	Kang SS, Keasey MP, Arnold SA, Reid R, Geralds J and Hagg T: Endogenous CNTF mediates stroke-induced adult CNS neurogenesis in mice. Neurobiol Dis. 49:68–78. 2013. View Article : Google Scholar : PubMed/NCBI
19	Watt JA, Bone S, Pressler M, Cranston HJ and Paden CM: Ciliary neurotrophic factor is expressed in the magnocellular neurosecretory system of the rat in vivo: Evidence for injury- and activity-induced upregulation. Exp Neurol. 197:206–214. 2006. View Article : Google Scholar : PubMed/NCBI
20	Ip NY, McClain J, Barrezueta NX, Aldrich TH, Pan L, Li Y, Wiegand SJ, Friedman B, Davis S and Yancopoulos GD: The alpha component of the CNTF receptor is required for signaling and defines potential CNTF targets in the adult and during development. Neuron. 10:89–102. 1993. View Article : Google Scholar : PubMed/NCBI
21	Rudge JS, Li Y, Pasnikowski EM, Mattsson K, Pan L, Yancopoulos GD, Wiegand SJ, Lindsay RM and Ip NY: Neurotrophic factor receptors and their signal transduction capabilities in rat astrocytes. Eur J Neurosci. 6:693–705. 1994. View Article : Google Scholar : PubMed/NCBI
22	Levison SW, Hudgins SN and Crawford JL: Ciliary neurotrophic factor stimulates nuclear hypertrophy and increases the GFAP content of cultured astrocytes. Brain Res. 803:189–193. 1998. View Article : Google Scholar : PubMed/NCBI
23	Albrecht PJ, Dahl JP, Stoltzfus OK, Levenson R and Levison SW: Ciliary neurotrophic factor activates spinal cord astrocytes, stimulating their production and release of fibroblast growth factor-2, to increase motor neuron survival. Exp Neurol. 173:46–62. 2002. View Article : Google Scholar : PubMed/NCBI
24	Albrecht PJ, Murtie JC, Ness JK, Redwine JM, Enterline JR, Armstrong RC and Levison SW: Astrocytes produce CNTF during the remyelination phase of viral-induced spinal cord demyelination to stimulate FGF-2 production. Neurobiol Dis. 13:89–101. 2003. View Article : Google Scholar : PubMed/NCBI