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Differential proteomic analysis of respiratory failure in peripheral blood mononuclear cells using iTRAQ technology

  • Authors:
    • Guoping Sun
    • Cuihui Cao
    • Wenbiao Chen
    • Yang Zhang
    • Yong Dai
  • View Affiliations / Copyright

    Affiliations: Central Laboratory of Pingshan New District People's Hospital of Shenzhen, Shenzhen, Guangdong 518020, P.R. China, Clinical Medical Research Center, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 573-577
    |
    Published online on: March 17, 2016
       https://doi.org/10.3892/br.2016.633
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Abstract

Respiratory failure (RF) is a state in which the respiratory system fails by its gas exchange functions. Failure of the lung, which is caused by all types of lung diseases, leads to hypoxaemia with type I respiratory failure. Failure of the pump leads to hypercapnia or type II respiratory failure. Using isobaric tags for relative and absolute quantification (iTRAQ) technology to identify and quantify the total proteins in peripheral blood mononuclear cells (PBMCs) of RF patients and identify the differentially expressed proteome. The present study analyzed the total proteins in the PBMCs of RF patients and healthy controls using the eight‑plex iTRAQ added with strong cation‑exchange chromatography and liquid chromatography coupled with tandem mass spectrometry. The differentially expressed proteins were identified by MASCOT. A total of 4,795 differentially expressed proteins were identified, and 403 proteins were upregulated and 421 were downregulated. Among them, 4 proteins were significantly differentially expressed, which were upregulated KIAA1520 protein and γ fibrinogen type B (AA at 202) and downregulated chain A, crystal structure of recombinant human platelet factor 4 and myosin regulatory light polypeptide 9. iTRAQ technology is suitable for identifying and quantifying the proteome in the PBMCs of RF patients. The differentially expressed proteins of RF patients have been identified in the present study, and further research of the molecular mechanism of the differentially expressed proteins is required to clarify the pathogenesis and identify novel biomarkers of RF.
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1 

Roussos C and Koutsoukou A: Respiratory failure. Eur Respir J Suppl. 47:3s–14s. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Roussos C and Macklem PT: The respiratory muscles. N Engl J Med. 307:786–797. 1982. View Article : Google Scholar : PubMed/NCBI

3 

Burt CC and Arrowsmith JE: Respiratory failure. Surgery. 27:475–479. 2009.

4 

Chang X, Cui Y, Zong M, Zhao Y, Yan X, Chen Y and Han J: Identification of proteins with increased expression in rheumatoid arthritis synovial tissues. J Rheumatol. 36:872–880. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Gibson DS, Blelock S, Curry J, Finnegan S, Healy A, Scaife C, McAllister C, Pennington S, Dunn M and Rooney M: Comparative analysis of synovial fluid and plasma proteomes in juvenile arthritis - proteomic patterns of joint inflammation in early stage disease. J Proteomics. 72:656–676. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Dai Y, Hu C, Huang Y, Huang H, Liu J and Lv T: A proteomic study of peripheral blood mononuclear cells in systemic lupus erythematosus. Lupus. 17:799–804. 2008. View Article : Google Scholar : PubMed/NCBI

7 

DeSouza LV, Grigull J, Ghanny S, Dubé V, Romaschin AD, Colgan TJ and Siu KW: Endometrial carcinoma biomarker discovery and verification using differentially tagged clinical samples with multidimensional liquid chromatography and tandem mass spectrometry. Mol Cell Proteomics. 6:1170–1182. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Al Badaai Y, DiFalco MR, Tewfik MA and Samaha M: Quantitative proteomics of nasal mucus in chronic sinusitis with nasal polyposis. J Otolaryngol Head Neck Surg. 38:381–389. 2009.PubMed/NCBI

9 

Zhou L, Beuerman RW, Chan CM, Zhao SZ, Li XR, Yang H, Tong L, Liu S, Stern ME and Tan D: Identification of tear fluid biomarkers in dry eye syndrome using iTRAQ quantitative proteomics. J Proteome Res. 8:4889–4905. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Hergenroeder G, Redell JB, Moore AN, Dubinsky WP, Funk RT, Crommett J, Clifton GL, Levine R, Valadka A and Dash PK: Identification of serum biomarkers in brain-injured adults: Potential for predicting elevated intracranial pressure. J Neurotrauma. 25:79–93. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Bantscheff M, Schirle M, Sweetman G, Rick J and Kuster B: Quantitative mass spectrometry in proteomics: A critical review. Anal Bioanal Chem. 389:1017–1031. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Li X, Hu B, Ding J and Chen H: Rapid characterization of complex viscous samples at molecular levels by neutral desorption extractive electrospray ionization mass spectrometry. Nat Protoc. 6:1010–1025. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Couttas TA, Raftery MJ, Erce MA and Wilkins MR: Monitoring cytoplasmic protein complexes with blue native gel electrophoresis and stable isotope labelling with amino acids in cell culture: Analysis of changes in the 20S proteasome. Electrophoresis. 32:1819–1823. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Albaum SP, Hahne H, Otto A, Haußmann U, Becher D, Poetsch A, Goesmann A and Nattkemper TW: A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: An application study. Proteome Sci. 9:302011. View Article : Google Scholar : PubMed/NCBI

15 

Hergenroeder G, Redell JB, Moore AN, Dubinsky WP, Funk RT, Crommett J, Clifton GL, Levine R, Valadka A and Dash PK: Identification of serum biomarkers in brain-injured adults: Potential for predicting elevated intracranial pressure. J Neurotrauma. 25:79–93. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Matta A, DeSouza LV, Shukla NK, Gupta SD, Ralhan R and Siu KW: Prognostic significance of head-and-neck cancer biomarkers previously discovered and identified using iTRAQ-labeling and multidimensional liquid chromatography-tandem mass spectrometry. J Proteome Res. 7:2078–2087. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Nagase T, Kikuno R, Ishikawa K, Hirosawa M and Ohara O: Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7:143–150. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Crabtree GR and Kant JA: Organization of the rat gamma-fibrinogen gene: Alternative mRNA splice patterns produce the gamma A and gamma B (gamma ') chains of fibrinogen. Cell. 31:159–166. 1982. View Article : Google Scholar : PubMed/NCBI

19 

Fornace AJ Jr, Cummings DE, Comeau CM, Kant JA and Crabtree GR: Structure of the human gamma-fibrinogen gene. Alternate mRNA splicing near the 3′ end of the gene produces gamma A and gamma B forms of gamma-fibrinogen. J Biol Chem. 259:12826–12830. 1984.PubMed/NCBI

20 

Song YJ, Zhou ZH, Liu YK, Rao SM and Huang YJ: Prothrombotic state in senile patients with acute exacerbations of chronic obstructive pulmonary disease combined with respiratory failure. Exp Ther Med. 5:1184–1188. 2013.PubMed/NCBI

21 

Xu J, Gao XP, Ramchandran R, Zhao YY, Vogel SM and Malik AB: Nonmuscle myosin light-chain kinase mediates neutrophil transmigration in sepsis-induced lung inflammation by activating beta2 integrins. Nat Immunol. 9:880–886. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sun G, Cao C, Chen W, Zhang Y and Dai Y: Differential proteomic analysis of respiratory failure in peripheral blood mononuclear cells using iTRAQ technology. Biomed Rep 4: 573-577, 2016.
APA
Sun, G., Cao, C., Chen, W., Zhang, Y., & Dai, Y. (2016). Differential proteomic analysis of respiratory failure in peripheral blood mononuclear cells using iTRAQ technology. Biomedical Reports, 4, 573-577. https://doi.org/10.3892/br.2016.633
MLA
Sun, G., Cao, C., Chen, W., Zhang, Y., Dai, Y."Differential proteomic analysis of respiratory failure in peripheral blood mononuclear cells using iTRAQ technology". Biomedical Reports 4.5 (2016): 573-577.
Chicago
Sun, G., Cao, C., Chen, W., Zhang, Y., Dai, Y."Differential proteomic analysis of respiratory failure in peripheral blood mononuclear cells using iTRAQ technology". Biomedical Reports 4, no. 5 (2016): 573-577. https://doi.org/10.3892/br.2016.633
Copy and paste a formatted citation
x
Spandidos Publications style
Sun G, Cao C, Chen W, Zhang Y and Dai Y: Differential proteomic analysis of respiratory failure in peripheral blood mononuclear cells using iTRAQ technology. Biomed Rep 4: 573-577, 2016.
APA
Sun, G., Cao, C., Chen, W., Zhang, Y., & Dai, Y. (2016). Differential proteomic analysis of respiratory failure in peripheral blood mononuclear cells using iTRAQ technology. Biomedical Reports, 4, 573-577. https://doi.org/10.3892/br.2016.633
MLA
Sun, G., Cao, C., Chen, W., Zhang, Y., Dai, Y."Differential proteomic analysis of respiratory failure in peripheral blood mononuclear cells using iTRAQ technology". Biomedical Reports 4.5 (2016): 573-577.
Chicago
Sun, G., Cao, C., Chen, W., Zhang, Y., Dai, Y."Differential proteomic analysis of respiratory failure in peripheral blood mononuclear cells using iTRAQ technology". Biomedical Reports 4, no. 5 (2016): 573-577. https://doi.org/10.3892/br.2016.633
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