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Article Open Access

Clinical efficacy of the highly sensitive hepatitis C virus RNA quantitative assay in patients with relapse following interferon‑based therapy with second‑generation direct‑acting antivirals

  • Authors:
    • Toru Ishikawa
    • Satoshi Abe
    • Takayuki Watanabe
    • Yujiro Nozawa
    • Tomoe Sano
    • Akito Iwanaga
    • Keiichi Seki
    • Terasu Honma
    • Toshiaki Yoshida
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology and Hepatology, Saiseikai Niigata Daini Hospital, Niigata 950‑1104, Japan
    Copyright: © Ishikawa et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 664-666
    |
    Published online on: April 19, 2016
       https://doi.org/10.3892/br.2016.660
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Abstract

For refractory chronic hepatitis C, interferon (IFN)‑based triple‑agent combination therapy with second‑generation direct‑acting antivirals (DAAs) has been established as the standard treatment method. The rate of decrease in the viral load and the negative conversion of hepatitis C virus (HCV) RNA in the early phase following treatment initiation are considered important factors for predicting the therapeutic outcome. In the present study, the Roche Cobas AmpliPrep/COBAS TaqMan (CAP/CTM) HCV v2.0 assay and the AccuGENE m‑HCV RNA quantitative assay [Abbott RealTime HCV (ART) assay] were analyzed for their clinical efficacy and ability to predict therapeutic outcomes in the early phase in patients with relapse following IFN‑based second‑generation DAA therapy. Of the 56 patients who received IFN‑based second‑generation DAA therapy since December 2013, 6 achieved an end‑of‑treatment response (ETR), but subsequently experienced relapse. In these 6 patients, fluctuations in viral loads in the early phase detected by the CAP/CTM and ART assays were compared. At 4 weeks after treatment initiation, 4 of the 6 patients were diagnosed as negative by the CAP/CTM assay, whereas 2 of these 4 patients were not identified as negative by the ART assay. Of the 2 patients, one was signal‑positive with an HCV RNA load <1.08 Log IU/ml, and the other patient had a viral load of 1.12 Log IU/ml. At 8 weeks after treatment initiation, 1 patient was found to be negative by the CAP/CTM assay, but signal‑positive with a viral load <1.08 Log IU/ml by the ART assay. From 4 to 8 weeks after treatment initiation, 3 of the 6 patients appeared to be discrepant cases. In conclusion, of the 6 patients who achieved an ETR, 4 were determined to have achieved a rapid virological response (RVR) by the CAP/CTM assay, but may not have actually become negative. The ART assay is highly sensitive, has a wide measurement range, may be suitable for monitoring HCV RNA loads, and is expected to have an important role in providing a predictive marker for early therapeutic outcomes. In discrepant cases in which no RVR is proved by either assay, it was assumed important to consider continuation of treatment and to attempt to achieve a sustained virological response.
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Copy and paste a formatted citation
Spandidos Publications style
Ishikawa T, Abe S, Watanabe T, Nozawa Y, Sano T, Iwanaga A, Seki K, Honma T and Yoshida T: Clinical efficacy of the highly sensitive hepatitis C virus RNA quantitative assay in patients with relapse following interferon‑based therapy with second‑generation direct‑acting antivirals. Biomed Rep 4: 664-666, 2016.
APA
Ishikawa, T., Abe, S., Watanabe, T., Nozawa, Y., Sano, T., Iwanaga, A. ... Yoshida, T. (2016). Clinical efficacy of the highly sensitive hepatitis C virus RNA quantitative assay in patients with relapse following interferon‑based therapy with second‑generation direct‑acting antivirals. Biomedical Reports, 4, 664-666. https://doi.org/10.3892/br.2016.660
MLA
Ishikawa, T., Abe, S., Watanabe, T., Nozawa, Y., Sano, T., Iwanaga, A., Seki, K., Honma, T., Yoshida, T."Clinical efficacy of the highly sensitive hepatitis C virus RNA quantitative assay in patients with relapse following interferon‑based therapy with second‑generation direct‑acting antivirals". Biomedical Reports 4.6 (2016): 664-666.
Chicago
Ishikawa, T., Abe, S., Watanabe, T., Nozawa, Y., Sano, T., Iwanaga, A., Seki, K., Honma, T., Yoshida, T."Clinical efficacy of the highly sensitive hepatitis C virus RNA quantitative assay in patients with relapse following interferon‑based therapy with second‑generation direct‑acting antivirals". Biomedical Reports 4, no. 6 (2016): 664-666. https://doi.org/10.3892/br.2016.660
Copy and paste a formatted citation
x
Spandidos Publications style
Ishikawa T, Abe S, Watanabe T, Nozawa Y, Sano T, Iwanaga A, Seki K, Honma T and Yoshida T: Clinical efficacy of the highly sensitive hepatitis C virus RNA quantitative assay in patients with relapse following interferon‑based therapy with second‑generation direct‑acting antivirals. Biomed Rep 4: 664-666, 2016.
APA
Ishikawa, T., Abe, S., Watanabe, T., Nozawa, Y., Sano, T., Iwanaga, A. ... Yoshida, T. (2016). Clinical efficacy of the highly sensitive hepatitis C virus RNA quantitative assay in patients with relapse following interferon‑based therapy with second‑generation direct‑acting antivirals. Biomedical Reports, 4, 664-666. https://doi.org/10.3892/br.2016.660
MLA
Ishikawa, T., Abe, S., Watanabe, T., Nozawa, Y., Sano, T., Iwanaga, A., Seki, K., Honma, T., Yoshida, T."Clinical efficacy of the highly sensitive hepatitis C virus RNA quantitative assay in patients with relapse following interferon‑based therapy with second‑generation direct‑acting antivirals". Biomedical Reports 4.6 (2016): 664-666.
Chicago
Ishikawa, T., Abe, S., Watanabe, T., Nozawa, Y., Sano, T., Iwanaga, A., Seki, K., Honma, T., Yoshida, T."Clinical efficacy of the highly sensitive hepatitis C virus RNA quantitative assay in patients with relapse following interferon‑based therapy with second‑generation direct‑acting antivirals". Biomedical Reports 4, no. 6 (2016): 664-666. https://doi.org/10.3892/br.2016.660
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