Detection of plasmid-mediated AmpC β-lactamase in Escherichia coli

Liu,Xiangqun; Liu,Yongrui

doi:10.3892/br.2016.661

June-2016 Volume 4 Issue 6

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June-2016 Volume 4 Issue 6

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Article Open Access

Detection of plasmid-mediated AmpC β-lactamase in Escherichia coli

Authors:
- Xiangqun Liu
- Yongrui Liu
View Affiliations / Copyright

Affiliations: Department of Respiration, Xuzhou City Hospital Affiliated to Xuzhou Medical College, Xuzhou, Jiangsu, P.R. China, Department of Respiration, Jining No.1 People's Hospital, Jining, Shandong, P.R. China

Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 687-690
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Published online on: April 21, 2016

https://doi.org/10.3892/br.2016.661
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Abstract

Escherichia coli (E. coli) is a common opportunistic pathogen for nosocomial infection. The aim of the study was to examine the phenotype, genotype and epidemiology of plasmid‑mediated AmpC β‑lactamases in E. coli. In total, 96 clinical isolates of repeated E. coli were collected from different hospitals between August and October 2012. Using a cefoxitin disk diffusion method to identify the phenotype of AmpC β‑lactamases in E. coli, the plasmid was extracted, and multiplex polymerase chain reaction (PCR) was used to determine the amp gene. The PCR products were purified and sequenced. Of the 96 isolates strains, 43 strains were cefoxitin‑resistant. Twelve (12.5%) isolates were detected to produce AmpC β‑lactamases with multiplex PCR, 11 strains carried DHA type ampC‑resistant genes, and one strain carried ACC type ampC‑resistant genes. In conclusion, the incidence of producing a plasmid‑mediated AmpC enzyme of E. coli strains was relatively high. Therefore, antibiotics such as imipenem, a carbapenem, potentially serve as the treatment of choice for the infection.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Detection of plasmid-mediated AmpC β-lactamase in Escherichia coli

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