An improved polymerase chain reaction-restriction fragment length polymorphism assay for the detection of a PON2 gene polymorphism

Duan,Xiaoran; Yang,Yongli; Wang,Tuanwei; Feng,Xiaolei; Yao,Wu; Yan,Zhen; Wang,Wei

doi:10.3892/br.2016.676

July-2016 Volume 5 Issue 1

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July-2016 Volume 5 Issue 1

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Article

An improved polymerase chain reaction-restriction fragment length polymorphism assay for the detection of a PON2 gene polymorphism

Authors:
- Xiaoran Duan
- Yongli Yang
- Tuanwei Wang
- Xiaolei Feng
- Wu Yao
- Zhen Yan
- Wei Wang
View Affiliations / Copyright

Affiliations: Department of Occupational and Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China, Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China
Pages: 133-135
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Published online on: May 13, 2016

https://doi.org/10.3892/br.2016.676
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Abstract

In recent research, it has been shown that there have been variants of rs12026 within the paraoxonase 2 (PON2) gene, which have been associated with cardiovascular disease, cerebrovascular disease, diabetes and other diseases. The isochizomers, such as the BsoFI enzyme, required for the detection of this polymorphism are expensive. Therefore, an improved and less expensive polymerase chain reaction (PCR)-restriction fragment length polymorphism method was established for the detection of the single‑nucleotide polymorphism rs12026 in the exon 5 of chromosome 7 of the human PON2 gene using the method of amplification‑created restriction site. Subsequent to assessing 302 individuals, the genotype frequencies were 68.9% for CC, 29.8% for CG and 1.3% for GG, and the allelic frequencies were 83.8% for C and 16.2% for G. The PCR results were confirmed by DNA sequencing. The χ2 test showed that the genotype and allele frequencies of PON2‑148 do not deviate from Hardy-Weinberg equilibrium, and the sequences of amplified products were consistent with the sequence published in GenBank with the exception of a mismatched base.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

An improved polymerase chain reaction-restriction fragment length polymorphism assay for the detection of a PON2 gene polymorphism

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