Effects of the Sijunzi decoction on the immunological function in rats with dextran sulfate-induced ulcerative colitis

Yu,Wangui; Lu,Bing; Zhang,Hengwen; Zhang,Yanxiang; Yan,Jin

doi:10.3892/br.2016.678

Effects of the Sijunzi decoction on the immunological function in rats with dextran sulfate-induced ulcerative colitis

Authors:
- Wangui Yu
- Bing Lu
- Hengwen Zhang
- Yanxiang Zhang
- Jin Yan
View Affiliations

Affiliations: Department of Physiology, Medical School of Yangtze University, Jingzhou, Hubei 434000, P.R. China, Department of Traditional Medicine, Medical School of Yangtze University, Jingzhou, Hubei 434000, P.R. China, Department of Laboratory, Medical School of Yangtze University, Jingzhou, Hubei 434000, P.R. China
Published online on: May 16, 2016 https://doi.org/10.3892/br.2016.678
Pages: 83-86

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The present study investigated the effects of the Sijunzi decoction (SJZD) at various dosages on the immunological function of rats with 3% dextran sulfate sodium (DSS; molecular weight 5,000)‑induced ulcerative colitis (UC). A total of 40 male Wistar rats were randomly divided into 5 groups: Normal, model, low‑dose SJZD, moderate‑dose SJZD and high‑dose SJZD groups. The 3% DSS was intragastrically administered for 7 consecutive days in order to induce the UC model. The normal group consumed distilled water. Subsequently, SJZD (5.0, 10.0 and 30.0 g/kg) was intragastrically administered, and scores of the disease activity index (DAI) were calculated. After 2 weeks, all the rats were sacrificed. Scores of the colon mucosa damage index (CMDI) were evaluated; and secretory immunoglobulin A (sIgA) and interleukin-2 (IL-2) were measured in intestinal tissue by ELISA assays. The model group rats had ulcers, hyperemia and interstitial edema and infiltrated inflammatory cells. SJZD attenuated the severity of the gross lesions and reduced the histopathological injuries. Compared with the normal group, DAI and CMDI were significantly increased (P<0.01), and levels of determined sIgA in the intestinal mucosa and IL‑2 in the intestinal tissue were significantly decreased (P<0.05) in the model group. Compared with the model group, moderate and high doses of SJZD showed a restoration effect on all the aforementioned indexes, and the high dose was the most effective. In conclusion, SJZD can ameliorate inflammation in DSS‑induced UC rats. The mechanism is most likely due to enhancing intestinal local immunity.

View Figures

View References

1	Hisamatsu T, Kanai T, Mikami Y, Yoneno K, Matsuoka K and Hibi T: Immune aspects of the pathogenesis of inflammatory bowel disease. Pharmacol Ther. 137:283–297. 2013. View Article : Google Scholar : PubMed/NCBI
2	Siegmund B: Management of mild-to-moderate ulcerative colitis. Dig Dis. 33(Suppl 1): 90–94. 2015. View Article : Google Scholar : PubMed/NCBI
3	Seo GS and Chae SC: Biological therapy for ulcerative colitis: An update. World J Gastroenterol. 20:13234–13238. 2014. View Article : Google Scholar : PubMed/NCBI
4	Jiang XL and Cui HF: An analysis of 10218 ulcerative colitis cases in China. World J Gastroenterol. 8:158–161. 2002. View Article : Google Scholar : PubMed/NCBI
5	Park SJ, Kim WH and Cheon JH: Clinical characteristics and treatment of inflammatory bowel disease: A comparison of Eastern and Western perspectives. World J Gastroenterol. 20:11525–11537. 2014. View Article : Google Scholar : PubMed/NCBI
6	Russel MG: Changes in the incidence of inflammatory bowel disease: What does it mean? Eur J Intern Med. 11:191–196. 2000. View Article : Google Scholar : PubMed/NCBI
7	Liu L, Han L, Wong DY, Yue PY, Ha WY, Hu YH, Wang PX and Wong RN: Effects of Si-Jun-Zi decoction polysaccharides on cell migration and gene expression in wounded rat intestinal epithelial cells. Br J Nutr. 93:21–29. 2005. View Article : Google Scholar : PubMed/NCBI
8	Yu X, Cui Z, Zhou Z, Shan T, Li D and Cui N: Si-jun-zi decoction treatment promotes the restoration of intestinal function after obstruction by regulating intestinal homeostasis. Evid Based Complement Alternat Med. 2014:9285792014. View Article : Google Scholar : PubMed/NCBI
9	Wang X, Yang J, Cao Q and Tang J: Therapeutic efficacy and mechanism of water-soluble extracts of Banxiaxiexin decoction on BALB/c mice with oxazolone-induced colitis. Exp Ther Med. 8:1201–1204. 2014.PubMed/NCBI
10	Myers KJ, Murthy S, Flanigan A, Witchell DR, Butler M, Murray S, Siwkowski A, Goodfellow D, Madsen K and Baker B: Antisense oligonucleotide blockade of tumor necrosis factor-alpha in two murine models of colitis. J Pharmacol Exp Ther. 304:411–424. 2003. View Article : Google Scholar : PubMed/NCBI
11	Katti MK: Assessment of serum IL-1, IL-2 and IFN-γ levels in untreated pulmonary tuberculosis patients: Role in pathogenesis. Arch Med Res. 42:199–201. 2011. View Article : Google Scholar : PubMed/NCBI
12	Minaiyan M, Ghassemi-Dehkordi N, Mahzouni P and Ahmadi NS: Anti-inflammatory effect of Helichrysum oligocephalum DC extract on acetic acid - induced acute colitis in rats. Adv Biomed Res. 3:872014. View Article : Google Scholar : PubMed/NCBI
13	Kaneko A, Kono T, Miura N, Tsuchiya N and Yamamoto M: Preventive effect of TU-100 on a type-2 model of colitis in mice: Possible involvement of enhancing adrenomedullin in intestinal epithelial cells. Gastroenterol Res Pract. 2013:3840572013. View Article : Google Scholar : PubMed/NCBI
14	Motavallian A, Minaiyan M, Rabbani M, Andalib S and Mahzouni P: Involvement of 5HT3 receptors in anti-inflammatory effects of tropisetron on experimental TNBS-induced colitis in rat. Bioimpacts. 3:169–176. 2013.PubMed/NCBI
15	Okayama M, Tsubouchi R, Nishio H, Kato S and Takeuchi K: Protective effect of intra-rectal administration of rebamipide on dextran sulfate sodium-induced rat colitis. Digestion. 70:240–249. 2004. View Article : Google Scholar : PubMed/NCBI
16	Randhawa PK, Singh K, Singh N and Jaggi AS: A review on chemical-induced inflammatory bowel disease models in rodents. Korean J Physiol Pharmacol. 18:279–288. 2014. View Article : Google Scholar : PubMed/NCBI
17	Owaga E, Hsieh RH, Mugendi B, Masuku S, Shih CK and Chang JS: Th17 cells as potential probiotic therapeutic targets in inflammatory bowel diseases. Int J Mol Sci. 16:20841–20858. 2015. View Article : Google Scholar : PubMed/NCBI
18	Tavakkoli H, Haghdani S, Adilipour H, Daghaghzadeh H, Minakari M, Adibi P, Ahmadi K and Emami MH: Serologic celiac disease in patients with inflammatory bowel disease. J Res Med Sci. 17:154–158. 2012.PubMed/NCBI
19	Greenbaum E, Engelhard D, Levy R, Schlezinger M, Morag A and Zakay-Rones Z: Mucosal (SIgA) and serum (IgG) immunologic responses in young adults following intranasal administration of one or two doses of inactivated, trivalent anti-influenza vaccine. Vaccine. 22:2566–2577. 2004. View Article : Google Scholar : PubMed/NCBI
20	Liaw FP, Lau LC, Lim AS, Lim TH, Lee GY and Tien SL: CpG oligonucleotide and interleukin 2 stimulation enables higher cytogenetic abnormality detection rates than 12-o-tetradecanolyphorbol-13-acetate in Asian patients with B-cell chronic lymphocytic leukemia (B-CLL). Int J Hematol. 100:545–553. 2014. View Article : Google Scholar : PubMed/NCBI
21	Cho JH, Boyman O, Kim HO, Hahm B, Rubinstein MP, Ramsey C, Kim DM, Surh CD and Sprent J: An intense form of homeostatic proliferation of naive CD8+ cells driven by IL-2. J Exp Med. 204:1787–1801. 2007. View Article : Google Scholar : PubMed/NCBI
22	Yu DH, Bao YM, An LJ and Yang M: Protection of PC12 cells against superoxide-induced damage by isoflavonoids from Astragalus mongholicus. Biomed Environ Sci. 22:50–54. 2009. View Article : Google Scholar : PubMed/NCBI
23	Xie F, Sakwiwatkul K, Zhang C, Wang Y, Zhai L and Hu S: Atractylodis macrocephalae Koidz. polysaccharides enhance both serum IgG response and gut mucosal immunity. Carbohydr Polym. 91:68–73. 2013. View Article : Google Scholar : PubMed/NCBI