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Article

Mechanism of chlorogenic acid treatment on femoral head necrosis and its protection of osteoblasts

  • Authors:
    • Mingjuan Zhang
    • Xianda Hu
  • View Affiliations / Copyright

    Affiliations: Guangzhou Vocational College of Science and Technology, Guangzhou, Guangdong 510550, P.R. China
  • Pages: 57-62
    |
    Published online on: May 17, 2016
       https://doi.org/10.3892/br.2016.679
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Abstract

The aim of the present study was to investigate the therapeutic effect of chlorogenic acid on hormonal femoral head necrosis and its protection of osteoblasts. The study established a femoral head necrosis model in Wistar rats using Escherichia coli endotoxin and prednisolone acetate. The rats were divided into five groups and were treated with different concentrations of chlorogenic acid (1, 10 and 20 mg/kg). The main detected indicators were the blood rheology, bone mineral density, and the hydroxyproline and hexosamine  (HOM) contents. At a cellular level, osteoblasts were cultured and treated by drug‑containing serum. Subsequently, cell proliferation and the osteoblast cycle were measured using flow cytometry, and the protein expression levels of Bax and B‑cell lymphoma 2 (Bcl‑2) were detected using western blotting. Chlorogenic acid at a concentration of 20 mg/kg (high‑dose) enhanced the bone mineral density of the femoral head and femoral neck following ischemia. Simultaneously, blood flow following the injection of prednisolone acetate was significantly improved, and the HOM contents of the high‑dose chlorogenic acid group were significantly different. The results from the flow cytometry analysis indicated that chlorogenic acid can efficiently ameliorate hormone‑induced necrosis. The osteoblasts were isolated and cultured. The MTT colorimetric assay showed that chlorogenic acid at different densities can increase the proliferation capabilities of osteoblasts and accelerate the transition process of G0/G1 phase to S phase, as well as enhance mitosis and the regeneration of osteoblasts. Western blotting detection indicated that chlorogenic acid may prohibit the decrease of Bcl‑2 and the increase of Bax during apoptosis, thereby inhibiting osteoblast apoptosis and preventing the deterioration of femoral head necrosis. In conclusion, chlorogenic acid at the density of 20 mg/kg is effective in the treatment of hormonal femoral head necrosis, which may be applicable for future treatment.
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1 

Wang XS, Zhuang QY, Weng XS, Lin J, Jin J and Qian WW: Etiological and clinical analysis of osteonecrosis of the femoral head in Chinese patients. Chin Med J (Engl). 126:290–295. 2013.PubMed/NCBI

2 

Zhao DW and Hu YC: Chinese experts' consensus on the diagnosis and treatment of osteonecrosis of the femoral head in adults. Orthop Surg. 4:125–130. 2012. View Article : Google Scholar : PubMed/NCBI

3 

de Martel C, Ferlay J, Franceschi S, Vignat J, Bray F, Forman D and Plummer M: Global burden of cancers attributable to infections in 2008: A review and synthetic analysis. Lancet Oncol. 13:607–615. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Kim HA and Blanco FJ: Cell death and apoptosis in osteoarthritic cartilage. Curr Drug Targets. 8:333–345. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Rahman WA, Garbuz DS and Masri BA: Total hip arthroplasty in steroid-induced osteonecrosis: Early functional and radiological outcomes. Can J Surg. 56:41–46. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Helbig L, Simank HG, Kroeber M, Schmidmaier G, Grützner PA and Guehring T: Core decompression combined with implantation of a demineralised bone matrix for non-traumatic osteonecrosis of the femoral head. Arch Orthop Trauma Surg. 132:1095–1103. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Liu HY, Wu AT, Tsai CY, Chou KR, Zeng R, Wang MF, Chang WC, Hwang SM, Su CH and Deng WP: The balance between adipogenesis and osteogenesis in bone regeneration by platelet-rich plasma for age-related osteoporosis. Biomaterials. 32:6773–6780. 2011. View Article : Google Scholar : PubMed/NCBI

8 

James AW, Leucht P, Levi B, Carre AL, Xu Y, Helms JA and Longaker MT: Sonic Hedgehog influences the balance of osteogenesis and adipogenesis in mouse adipose-derived stromal cells. Tissue Eng Part A. 16:2605–2616. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Erken HY, Ofluoglu O, Aktas M, Topal C and Yildiz M: Effect of pentoxifylline on histopathological changes in steroid-induced osteonecrosis of femoral head: Experimental study in chicken. Int Orthop. 36:1523–1528. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Vélez R, Hernández-Fernández A, Caminal M, Vives J, Soldado F, Fernández A, Pla A and Aguirre M: Treatment of femoral head osteonecrosis with advanced cell therapy in sheep. Arch Orthop Trauma Surg. 132:1611–1618. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Stuss M, Rieske P, Cegłowska A, Stêpień-Kłos W, Liberski PP, Brzeziańska E and Sewerynek E: Assessment of OPG/RANK/RANKL gene expression levels in peripheral blood mononuclear cells (PBMC) after treatment with strontium ranelate and ibandronate in patients with postmenopausal osteoporosis. J Clin Endocrinol Metab. 98:E1007–E1011. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Kim BS, Jung JS, Jang JH, Kang KS and Kang SK: Nuclear Argonaute 2 regulates adipose tissue-derived stem cell survival through direct control of miR10b and selenoprotein N1 expression. Aging Cell. 10:277–291. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Youm YS, Lee SY and Lee SH: Apoptosis in the osteonecrosis of the femoral head. Clin Orthop Surg. 2:250–255. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Hwang Y, Park J, Choi SH and Kim G: Traumatic and Non-traumatic osteonecrosis in the femoral head of a rabbit model. Lab Anim Res. 27:127–131. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Takano-Murakami R, Tokunaga K, Kondo N, Ito T, Kitahara H, Ito M and Endo N: Glucocorticoid inhibits bone regeneration after osteonecrosis of the femoral head in aged female rats. Tohoku J Exp Med. 217:51–58. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Gangji V and Hauzeur JP: Treating osteonecrosis with autologous bone marrow cells. Skeletal Radiol. 39:209–211. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Seamon J, Keller T, Saleh J and Cui Q: The pathogenesis of nontraumatic osteonecrosis. Arthritis (Egypt). 2012:6017632012.

18 

Xie XH, Wang XL, He YX, Liu Z, Sheng H, Zhang G and Qin L: Promotion of bone repair by implantation of cryopreserved bone marrow-derived mononuclear cells in a rabbit model of steroid-associated osteonecrosis. Arthritis Rheum. 64:1562–1571. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Vucic D: Apoptotic pathways as targets for therapeutic intervention. Current Cancer Drug Targets. 8:862008. View Article : Google Scholar : PubMed/NCBI

20 

Jung HS, Kim YH and Lee JW: Duration and magnitude of extracellular signal-regulated protein kinase phosphorylation determine adipogenesis or osteogenesis in human bone marrow-derived stem cells. Yonsei Med J. 52:165–172. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Imhoff BR and Hansen JM: Differential redox potential profiles during adipogenesis and osteogenesis. Cell Mol Biol Lett. 16:149–161. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang M and Hu X: Mechanism of chlorogenic acid treatment on femoral head necrosis and its protection of osteoblasts. Biomed Rep 5: 57-62, 2016.
APA
Zhang, M., & Hu, X. (2016). Mechanism of chlorogenic acid treatment on femoral head necrosis and its protection of osteoblasts. Biomedical Reports, 5, 57-62. https://doi.org/10.3892/br.2016.679
MLA
Zhang, M., Hu, X."Mechanism of chlorogenic acid treatment on femoral head necrosis and its protection of osteoblasts". Biomedical Reports 5.1 (2016): 57-62.
Chicago
Zhang, M., Hu, X."Mechanism of chlorogenic acid treatment on femoral head necrosis and its protection of osteoblasts". Biomedical Reports 5, no. 1 (2016): 57-62. https://doi.org/10.3892/br.2016.679
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang M and Hu X: Mechanism of chlorogenic acid treatment on femoral head necrosis and its protection of osteoblasts. Biomed Rep 5: 57-62, 2016.
APA
Zhang, M., & Hu, X. (2016). Mechanism of chlorogenic acid treatment on femoral head necrosis and its protection of osteoblasts. Biomedical Reports, 5, 57-62. https://doi.org/10.3892/br.2016.679
MLA
Zhang, M., Hu, X."Mechanism of chlorogenic acid treatment on femoral head necrosis and its protection of osteoblasts". Biomedical Reports 5.1 (2016): 57-62.
Chicago
Zhang, M., Hu, X."Mechanism of chlorogenic acid treatment on femoral head necrosis and its protection of osteoblasts". Biomedical Reports 5, no. 1 (2016): 57-62. https://doi.org/10.3892/br.2016.679
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